查询词典 staining

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

PPARγ expression in PAN-treated rats at 12 weeks was present in podocytes and in mesangial cells in segmentally sclerotic glomeruli, with occasional parietal epithelial cell staining.

给予PAN后，PPARγ可以在节段硬化小球的足细胞和系膜细胞上表达，部分在壁层上皮细胞表达。6周后给予吡咯列酮治疗，可以减少系膜区的PPARγ染色，但足细胞上的染色明显增强，伴有小球硬化明显减轻。

Four weeks after treatment, immunohistochemistry staining and VIDAS analysis were applied to semiquantitatively analyze the expression of collagen Ⅱ.

随着分子生物学的迅速发展，基因治疗为我们在细胞、分子水平上延缓或逆转椎间盘退变提供了可能。

All cases were immunostained using an antibody directed toward PAX5 and immunoreactivity was scored semiquantitatively according to percentage of nuclear staining.

所有病例都直接做了PAX5免疫染色，并依据瘤细胞核的阳性表达率，我们半定量地给它们评分。

Methods The routine examination of serous membrane effusion are based on smearing and WrightGiemsa staining,the character of cells were evaluated by veteran in the field of cytodiagnosis.

浆膜腔积液常规检查的制片采用推片法，涂片染色采用瑞吉复合染色法，通过有经验的细胞学学者鉴定细胞性质。

Methods The routine examination of serous membrane effusion are based on smearing and Wright??Giemsa staining,the character of cells were evaluated by veteran in the field of cytodiagnosis.

浆膜腔积液常规检查的制片采用推片法，涂片染色采用瑞吉复合染色法，通过有经验的细胞学学者鉴定细胞性质。

Cryptosporidium oocysts were separated from the faeces in a cattle farm and identified as Cryptosporidium muris according to their morphology, structure arid size after a sheather\'s floating and an auramine-phenol and acid-fast-staining.

从某奶牛场分离出隐孢子虫卵囊，用金胺酚-改良抗酸染色及饱和蔗糖漂浮后，通过显微镜检，根据其形态、结构、大小等特性鉴定为鼠隐孢子虫。

METHODSThe venous blood with no sibship was taken from 261 cases (160 of male, 101 of female ) of KoreanChinese in Yanbian area, and the analysis of DXS7132 short tandem STR locus was performed by using PCR, polyacylamide gel electrophoresis and silver staining.

方法］选择261名(男性为160名，女性为101名)延边地区朝鲜族无血缘关系的个体静脉血，提取DNA，经PCR扩增、聚丙烯酰胺凝胶电泳及银染显色等，对朝鲜族人群DXS7132基因座的基因多态性进行分析。

METHODSThe venous blood with no sibship was taken from 294 cases (187 of male, 107 of female) of KoreanChinese nationality.The DXS9898 short tandem STR locus was performed by using the techniques of PCR, polyacylamide gel electrophoresis and silver staining.

方法］随机抽取294名延边地区无血缘关系的朝鲜族个体，其中男性为187名，女性为107名，采集静脉血，提取DNA，行PCR扩增，变性聚丙烯酰胺凝胶电泳及银染显色，行遗传基因多态性分析。

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established. In situ hybridization and irnmunohistochemieal staining were applied to detect the gene expression of TGFβ1 and PDGF-B in the alveolar type Ⅱ cells of primary culture and silicotic tissue.

分离培养正常成年大鼠肺泡Ⅱ型细胞，建立大鼠矽肺模型，用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF-B mRNA和蛋白的表达。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。