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Staining observation: The grafts were removed at 1, 2 and 3 months after transplantation, then prepared into sections, and observed by hematoxylin and eosin, Safranin'O and Masson's trichrome stainings.

染色观察：移植接种后1，2，3月取出移植物，切片，行苏木精-伊红、Safranin'0、Masson's trichrome染色观察。

The rabbit model of osteoarthritis was established according to Hulth's method. The rabbits were sacrificed at the 8th week after administration of Yanghe Decoction for 14 days, and then rabbit tibia articular cartilage was removed. Sections of the cartilage were stained with Safranin O for histological examination. The cartilage histological characteristics were observed according to the method of Mankin. Immunohistochemical staining was performed to investigate the expression of VEGF.

按照Hulth法建立膝骨关节炎模型，术后第5周起至第7周末，阳和汤组予混有阳和汤的颗粒饲料，正常组及模型组均给予普通饲料；术后第8周分别取各组胫骨平台关节软骨，采用番红O染色，进行Mankin评分；免疫组织化学染色测定关节软骨中VEGF表达的阳性指数。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红"O"染色呈强阳性。

RESULTS: HE and safranine O staining both showed no cellular structure in the matrix with only recesses left by the removed cells.

软骨蛋白聚糖免疫组织化学染色阳性，提示脱细胞关节软骨基质内仍存在软骨蛋白聚糖。

Methods Safranine staining was conducted for the detection of the forming ability of biofilm in 96-well plates.icaAD,icaBC,sar,agr and sigB was amplified by PCR.

方法96孔板番红染色法检测生物膜的形成，icaAD、icaBC、sar、agr和sigB的PCR扩增，并对产物进行测序和同源性比较。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上，加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组；将SD仔鼠软骨细胞复合于支架上，常规培养2周作为对照组。20只裸小鼠，左侧腋窝皮下均植入实验组细胞-支架复合物，右侧腋窝皮下均植入对照组细胞-支架复合物。8， 12周各处死10只，行甲苯胺蓝和番红O-固绿染色。

The cells of 2~ passage were used for proliferation kinetics studying by MTT colorimetric method.The pasagcd chondrocytes' phenotypes were as- sessed by safranine O staining and immunostaining for typeⅠ,Ⅱcollagens and aggrecan.Results For in vitro culturing of AHAC,human AB serum was superior to FBS,and the optimal concentration of human serum was 10%.But for FBS,20% was better,A similar result was got with or without the growth factors contained.

结果对于AHAC，人AB血清培养优于FBS；合适的人AB血为10%；合适的FBS浓度为20%；无血清培养细胞增殖非常缓慢；不加因子用人AB血清培养细胞梭形化明显，加用生长因子后与使用FBS在形态上一致。

A spectrophotometric method to measure the concentration of salicin in the water extract of willow bark was established based on the HCLO4 - Vanilin staining reaction with λmax of 520 nm.

以水为溶剂，50℃下超声提取柳树茎皮中的水杨苷，采用香草醛分光光度法，在吸收波长520nm下，建立水杨苷含量的测定方法。

After 24 h, samples were harvested and the scalding lesions were evaluated by HE staining.

伤后24 h取标本，经HE染色评价烫伤程度，并对烫伤的时间和程度作相关性分析。

Positive staining of Stro-1 was found in 7.6% of SCAP. Morphologically, PDLSCs were fusiform-shaped, with many short and long ramifications under a scanning electron microscope, and the secreted extracellular matrix were around them.

透射电镜下可见，第1～3代牙周膜干细胞及根尖牙乳头干细胞生长良好，胞浆内线粒体，高尔基体和粗面内质网丰富，随着培养时间的延长，分泌的细胞外基质增多。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。