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After Reiter staining, fibroblast-like cell morphology could be seen, and cells grew with vertex or whirlpool.

倒置显微镜下可见贴壁细胞呈圆形，有小的胞浆突起。

Then replate them onto feeder layer culture system (containing leukemia inhibitory factor) in order to passage and expand. Subsequently we identified the expanding cells by alkaline phosphatase staining, karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs.

以分离培养的细胞作为供体细胞，近交系C57BL/6J为受体囊胚供者，采用囊胚注射法构建嵌合胚，体外恢复培养后移植进动情期子宫中，结果生出了黑白杂合的嵌合鼠。

Objective To evaluate a kind of modified reticulocyte staining solution.

目的 评价一种改良的网织红细胞染色液。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

The change of cell energy metabolism was observed by mitochondria staining with rhodamine.

已有研究报道，在离体模型上，尼可地尔对缺血再灌注心肌有保护作用。

The percentage of collagen type ⅱ and alcian blue positive stained rgcs decreased with increasing passage number. only the roundish cells of the 5th passage retained positive for collagen typeⅱ, and alcian blue staining of 3rd passage was negative, while α sma positive stained rgcs increased gradually.

随着传代次数的增加，阿尔新兰和ⅱ型胶原阳性染色的细胞比例迅速下降，第3代以后的rgcs阿尔新兰染色均为阴性，第5代rgcs也仅有圆形细胞仍保持了ⅱ型胶原阳性染色，而α sma阳性染色细胞的比例则不断增加。

To prevent your neck from staining your shirt collar, wipe your neck with rubbing alcohol each morning before you dress.

为了防止你的脖子被衬衫领子沾染，每天走上穿衣服前可以用酒精擦拭脖子。

Safranin O staining showed strong positive reaction, suggested that there were rich glycosaminoglycan in matrix.

Safranin'O染色呈强阳性反应，表明基质中富含糖胺多糖。

Methods :The Alcianblue Safranin and immunohistochemical staining were employed to observe the density of MC, PCNA and oncogene bcl-2 expression in the specimens of 74 cases with gastric cancer.

采用阿尔辛蓝-沙红及免疫组织化学染色法，同步检测74例胃癌标本中的肥大细胞数及PCNA、bcl-2基因蛋白的表达。

Wash with running water and again place the smear on the staining rack. Overlay the surface with safranin counterstain for 20 seconds. Wash with running water.

以流水冲洗后，再将玻片放置於染色架上，以safranin覆盖20秒以进行对比染色，再以流水冲洗

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。