查询词典 staining

Staining observation on complex mesochondrium of marrow-derived mesenchymal stem cells-TCP: It was found with TB staining that the complex of marrow-derived mesenchymal stem cells-TCP inside the joint cavity obviously had positive staining, which indicated that there were glycosidoprotein base formed.

骨髓基质干细胞-磷酸三钙复合体软骨基质特染观察结果：甲苯胺蓝染色发现，植入关节腔的骨髓基质干细胞-磷酸三钙复合体有明显阳性染色，表明有糖蛋白基质形成。

Primary cultured chondrocytes are poly-angle, cytoplasm-rich, and their nuclei are either round or oval with clear necleole. Metachromatic and alcian blue positive staining in primary cultured chondrocytes was observed. Intercellular matrix was anti-collagen type Ⅱ staining but not anti-collagen type Ⅰ staining by IHC assay.

原代培养软骨细胞呈多角型，胞质丰富，胞核成圆形或椭圆型，核仁清楚，甲苯胺蓝呈异染性，阿尔新蓝8Gx 染色阳性，细胞外基质Ⅱ型胶原免疫组化染色阳性，Ⅰ型胶原染色阴性。

MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标：倒置相差显微镜下观察骨髓间充质干细胞的生长状态，流式细胞仪检测细胞表面标志物的表达，采用碱性磷酸酶染色鉴定成骨能力，以油红O染色鉴定成脂能力，以甲苯胺蓝染色鉴定成软骨能力。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后，碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性；成脂诱导后油红染色呈阳性，有一定数量的脂滴形成。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

In cells that were positive for K3, weakly positive p63 staining was observed. After airlifting, involucrin showed positive staining in cells on the upper part of the cell sheet while staining for connexin 43 was observed in the basal layer.

暴露于气液面后，培养的角膜缘上皮细胞形成3-5层结构，外皮蛋白在外层细胞表达阳性，连接蛋白43在基底层的细胞中表达，而K3阳性的细胞很稀疏。

The lung was fixed in 10% formalin solution and embedded in paraffin for Haematoxyffin-eosin staining to observed the form of embolisms, Masson staining to assess collagen and fibrin/fibrinogen distribution and Toluidine blue staining was to observe mast cell distribution.

以10%甲醛固定肺组织，制成组织切片，行苏木精－伊红染色观察血栓的形态，Masson染色观察栓塞血管的胶原纤维分布，甲苯胺蓝染色观察血管栓塞处肥大细胞分布。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

At the third passage, BMSCs showed the typical polar swirl morphology. BMSCs were negative for CD34 and CD45, but positive for CD29 and CD44. Following induction, alkaline phosphatase staining, Oil red staining and toluidine blue staining produced a strong reaction in cells.

第3代骨髓间充质干细胞形态单一均匀，呈典型的极性漩涡状生长，不表达造血前体细胞标志抗原CD34和白细胞标志抗原CD45，表达整合素家族成员CD29和黏附分子CD44，经诱导后碱性磷酸酶染色、油红O染色和甲苯胺蓝染色均呈阳性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。