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In vivo: to examine oncogenicity by heterotransplantation into nude mice in situ and pathological characteristics with HE and immunohistochemistry staining.

2体内实验研究：裸鼠原位接种检测该细胞株的致瘤性，组织化学染色观察其病理学特性。

METHODS: Liver fibrosis models were established in 24 SD rats by intragastric administration (7 g/kg) of alcohol (56°, 560 mL/L) every morning, and 10 normal rats served as controls. The rats in model group were killed by exsanguination from femoral vein at 4, 12 and 24 wk. Pathological changes of liver tissues in rats were identified by Masson staining, and the expression of VEGF mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.

56度的白酒(560 mL/L)平均以7 g/kg的剂量每日早晨灌胃一次制备肝纤维化模型，灌胃4 wk、12 wk及24 wk采用股静脉放血法分别处死大鼠，观察肝脏病理变化并采用半定量逆转录聚合酶链反应检测VEGF mRNA的表达。

Methods Mesenchymal stem cells were collected from the femora of SD rats by density gradient centrifugation and identified by flow cytometric analysis and alkaline phosphatase staining.

方法采用梯度离心法，分离纯化SD大鼠的MSCs，流式细胞仪检测和碱性磷酸酶染色鉴定细胞类型。

Objective: To develope a new technique for bacterial flagella staining.

目的：发明一种新的细菌鞭毛镀银染色方法。

In human spermatozoa, the positive immunofluorescent staining of centrin protein was seen to be located in the basal body of flagellum and clearly in proximal and distal centrioles.

健康男性精子中Centrin免疫荧光阳性，位于鞭毛的基体部，显示近端和远端中心粒，而Cenexin只在一个中心粒上有明显阳性染色，表明该蛋白是人成熟精子中心体的一种重要结构成分。这一结果显示了人和啮齿目动物精子中心体的差异。

The double flourescent staining of apoptosis and expression of transforming growth factor β1 (TGF β1) were examined in liver tissue of animal pattern of ADR-injury by immunohistochemical and TUNEL,and the level of lipid peroxidation in serum was observed.

结果 阿霉素组大鼠肝组织可见细胞凋亡和肝细胞中转化生长因子β1的异常表达；阿霉素组大鼠血清中脂质过氧化物明显升高；亚硒酸钠对阿霉素的损伤有明显的保护作用。

Methods The double flourescent staining of apoptosis and Desmin was examined in heart tissue of Keshan disease by immunohistochemical and TUNEL,and the confocal microscope was used to observe the results.

应用原位末端标记法及免疫组化技术在亚急型和慢型克山病患者心肌石蜡包埋组织切片上进行细胞凋亡及Desmin双重荧光染色，并应用共聚焦显微镜观察结果。

Methods Using Wistar rats as animal pattem of ADR-induced myocardial injury and sodium selenite as protective agent.The double flourescent staining of apoptosis and expression of transforming growth factor β1 (TGF β1) were examined in liver tissue of animal pattern of ADR-injury by immunohistochemical and TUNEL,and the level of lipid peroxidation in serum was observed.

通过复制大鼠阿霉素性心肌损伤的动物模型，以亚硒酸钠作为保护因素，应用原位末端标记法和免疫组化技术检测大鼠肝细胞凋亡和肝细胞转化生长因子β1表达，并观察了大鼠血清中脂质过氧化含量。

At 18 and 24 hours after culture, types of cell death were quantitatively detected using fluorescein isothiocyanate and propidium iodide staining.

25%，50%，100%浓度的3种材料浸提液体外培养小鼠成纤维细胞L929 24 h后，采用流式细胞术碘化丙啶染色法检测L929细胞的凋亡率及细胞周期分布情况。

The expression of vegf165 was confirmed by fluoroscopy and immunohistochemical staining.

目前，vegf已被广泛应用于缺血性疾病的治疗。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。