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Disk height index of intervertebral disks on X-ray films, areas and average T2 relaxation time of high intensity zone of disks on T2-weight MRI and histological score on pathological sections （HE staining, Masson staining and safranin O-fast green staining） were measured at 2 months, 4 months and 6 months after operation.

术后2、4、6个月时对正常及模型椎间盘观察X线片上的椎间盘高度指数、MRIT2加权相上的椎间盘高信号区的面积和平均T2弛豫时间、椎间盘纤维环损伤区的形态学变化、组织病理切片（HE染色、Masson三色胶原染色和番红O-固绿染色）椎间盘组织形态学变化、生物化学变化和组织学评分等指标。

Staining results of ADSCs: The extracellular matrix Alcian blue staining, Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry were positive.

脂肪间充质干细胞诱导后的细胞化学染色结果：诱导后脂肪间充质干细胞胞外基质阿尔新兰染色、蕃红O/固绿染色、和Ⅱ型胶原免疫细胞化学着色阳性。

The opti mumreagent,which was selected out through comparing the staining effects of 4 staining reagents(I-IK coloration solution,improved phenol fuchsin,safranine staining solution and aceto carmine),was i mproved phenol fuchsin.

通过比较4种染色试剂(I-IK染液、改良苯酚品红、番红染液和醋酸洋红)的染色效果选出的最佳试剂为改良苯酚品红。

Samples were procured at 6 week after transplantation and studied by nerve silver staining and myelin basic protein histochemical staining and nerve growth factor receptor immunohistochemical staining.

6周后组织切片，行 HE染色、嗜银染色以及髓鞘碱性蛋白和抗神经生长因子受体抗体免疫组化检查。

Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

Mallory trichrism staining exhibited light blue samples. Eight weeks following repair, CT showed round blunt defect edges in the coronal position, which had bony pustute connection with gel materials. Density at the defect region significantly increased. 3D reconstruction suggested that defects became small. After hematoxylin-eosin staining, abundant fibrous connective tissue appeared in defect regions, with bony tissues. Mallory trichrism staining revealed that maroon mature bone tissues were found, surrounded by light blue chondroid tissues.

修复后8周，冠状位CT显示缺损边缘圆钝，与凝胶材料有骨性突起连接，缺损处密度增高明显，三维重建显示缺损范围较之前有所减小；标本苏木精-伊红染色后缺损部可见大量含血管成分的纤维结缔组织，有骨样组织结构形成，Mallory三色染色显示有褐红色成熟骨组织形成，其旁边有淡蓝色软骨样组织。

Mallory trichrism staining exhibited light blue samples. Eight weeks following repair, CT showed round blunt defect edges in the coronal position, which had bony pustute connection with gel materials. Density at the defect region significantly increased. 3D reconstruction suggested that defects became small. After hematoxylin-eosin staining, abundant fibrous connective tissue appeared in defect regions, with bony tissues. Mallory trichrism staining revealed that maroon mature bone tissues were found, surrounded by light blue chondroid tissues. Twelve weeks following repair, coronal defect regions were filled with callus. 3D reconstruction showed that defects were repaired. Defect region and surrounding bony tissues had bony connection, with thick bone trabecula and mature Haversian system.

修复后8周，冠状位CT显示缺损边缘圆钝，与凝胶材料有骨性突起连接，缺损处密度增高明显，三维重建显示缺损范围较之前有所减小；标本苏木精－伊红染色后缺损部可见大量含血管成分的纤维结缔组织，有骨样组织结构形成，Mallory三色染色显示有褐红色成熟骨组织形成，其旁边有淡蓝色软骨样组织修复后12周，冠状位CT显示缺损区基本被骨痂填满，三维重建示缺损基本修复；缺损区域和周围骨组织形成骨性结合，骨小梁粗大，哈弗氏系统成熟。

Staining extent and intensity were evaluated semiquantitatively and mean values for each parameter were calculated. Immunostaining with D2-40 showed positivity in 100% of skeletal myxoid chondrosarcomas, 96% of enchondromas, 95% of low-grade chondrosarcomas, 80% of chordoid meningiomas, and 75% of chordoid gliomas. Staining with S100 demonstrated diffuse, strong positivity in all (100%) chordoid gliomas, skeletal myxoid chondrosarcomas, low-grade chondrosarcomas, and enchondromas, 94% of chordomas, and 81% of extraskeletal myxoid chondrosarcomas, with focal, moderate staining in 40% of chordoid meningiomas.

我们半定量地评估了这些免疫染色的广度和强度，并且计算了它们各自的平均值。D2-40阳性表达于100%例骨的黏液样软骨肉瘤、96%例内生性软骨瘤、95%例低级别软骨肉瘤、80%例脊索样脑膜瘤和75%例脊索样胶质瘤。S100染色弥漫且强烈地表达于所有的（100%）脊索样胶质瘤、骨的黏液样软骨肉瘤、低级别软骨肉瘤和内生性软骨瘤，94%例脊索瘤，81%例骨外黏液样软骨肉瘤，还有，局灶性、中度表达于40%例黏液样脑膜瘤。

Results:17 rats were confirmed successful transplantation by MRI,the capacity of the tumor was 88.40±7.62mm3 on 8th day, 986.80±114.46mm3 on 16th day,the volume growth was 8-12 folds;on 8th day following transplantation,the tumor tissue was gray in color with fish homogen state without interior necrosis, the tumor cells were nest-distributed by HE staining,large dense staining of nucleus with manifest heteromorphism,tumor microvessel count increased by immunohistochemistry with buffy yellow staining, VEGF high expressed in the tumor cells with buffy yellow granule shape.

结果：17只大鼠经MRI检查证实均种植成功，肿瘤体积第8天为88.40±7.62mm3，第16天为986.80±114.46mm3，体积增长8-12倍之间；种植第8天，肿瘤组织呈灰白色、鱼肉均质状，内部未见坏死，HE染色肿瘤细胞呈巢状分布，胞核大浓染，异型性明显；免疫组织化学示肿瘤微血管数目较多，呈棕黄色染色，肿瘤细胞VEGF高表达，呈棕黄色细颗粒状。

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化，CD44免疫组化法分析ADSCs表面标志；用含成骨、成脂诱导培养基培养4周后，行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。