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Then the changes of cell shape and ultrastructure were observed by microscope and transmission electron microscopy, and the percent of cells in the G1 phase and SA- ft -galactosidase activity were measured with flow cytometry and cytochemistry staining, respectively.

从光镜、透射电镜观察细胞形态及超微结构的变化；流式细胞术分析细胞周期，计算G1期细胞的比例；SA-β-半乳糖苷酶的细胞化学染色，确定成功建立体外细胞衰老的模型。

This course is to include the following contents: 1.the history of cytogenetics, 2.the fine structure of chromosomes and staining technique, 3.the diversity and evolution of chromosome, 4.the relationship between plant breeding and cytogenesis on tropical vegetable production.

本课程提供大学部高年级及研究生研习。课程内容包括细胞遗传史，染色体之细微构造及染色技术，染色体之变异与演化，细胞遗传与植物育种之关联性等。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

They all took on brown staining in the cytoplasm and process.

这两种因子的阳性反应均定位于胞浆和突起。

488Anti-rat CD106 and Bis-Benzimide staining to calculate cytoplast purity. Mitochondrial membrane potential was detected by flow cytometry following labeling with Rhodamine123. RESULTS: The purity of cytoplasts was up to 95%.

488anti-rat CD106及Bis- Benzimide染色进行形态学观察，计算胞质体纯度；应用Rhodamine123标记后用流式细胞仪检测胞质体中线粒体膜势能。

Flow cytometry was used to measure the cells cycle kinetics of the treated cells. Hoechst33258/PI double staining was performed to evaluate the cell morphology. Western blotting was conducted to analyze the expressions of p53, Bcl 2 and Bax. Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells.

以胎盼蓝活细胞拒染法测定药物对细胞生长、增殖的抑制及IC50 ；以流式细胞术观察药物对细胞周期的影响；以荧光双染法（Hoechst33258/PI）观察药物对细胞形态学的影响；以Western blot法观察药物对细胞P53、Bcl 2、Bax蛋白表达的影响。

Objective: To observe the effect of decalcification on mast cell histochemical and immunohistochemical staining in rat cranial bone samples.

目的：探讨脱钙对鼠颅骨标本中肥大细胞组织化学与免疫组织化学染色影响。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

The hypoxic models mimic 8000 m high level were established in a high-altitude decompression chamber. Injecting the tracer of CB into the rat's lateral ventricles, we investigated the distribution of the distal CSF-CNs in the DR. The single and double immunohistochemistry staining with antibodies to Fos and CB were used to observe the expression of Fos in the distal CSF-CNs in the DR.

应用低压氧舱仿海拔8000米高空缺氧模型，采用侧脑室注射CB标记中缝背核内的远位触液神经元，并用Fos免疫组化和CB/Fos免疫组化双重染色方法显示Fos在中缝背核远位触液神经元内的表达情况。

Results: It was observed that projections from IN to hypothalamus passed through the superior cerebellar peduncle, crossed in its decussation and then many of them entered posterior hypothalamic area by iontophoresis of Dextran-Texas Red to cerebellar IN. Using the methods of iontophoresis of Fluoro-Ruby and double labelling staining, it was also observed that the neurons projecting to PH in cerebellar IN were glutamatergic neurons.

结果：小脑间位核电泳Dextran-TR后示踪可见间位核神经元发出离核纤维，行走于同侧小脑上脚中，在小脑上脚交叉处交叉到对侧，然后继续走行于小脑上脚中，到下丘脑后较多纤维终止于下丘脑后区；在下丘脑后区电泳Fluoro-Ruby后可逆行追踪到小脑间位核神经元，荧光双标法显示投射到下丘脑后区的小脑间位核神经元中有谷氨酸能神经元。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。