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Immunoprecipitation and cresyl fast violet staining were employed to detect the expression of relevant message proteins, activation and the death of the hippocampal neurons.results it was found that hpk1 was expressed in rat hippocampus neurons.conclusions administration of hpk1 antisense oligodeoxynucleotides can significantly protect hippocampus neurons from apoptosis without dose-dependence.

使用免疫沉淀和焦油紫染色法等技术检测相关信号蛋白的表达、活化水平及神经元细胞的死亡。结果在大鼠海马神经元中有hpk1的表达，脑室注射as-odns大鼠海马ca1区存活锥体细胞明显增多。结论 hpk1反义寡核苷酸对缺血/再灌注引起的海马神经元凋亡具有保护作用。

Basing on the four-vessel occlusion models,neuron apoptosis in CA1 region of hippocampus was detected by cresyl violet staining,the phosphorylation of Akt and Bad(ser136)were detected by immunoblotting.

以大鼠四肢动脉结扎脑缺血模型为基础，应用焦油紫染色法检测海马CA1区神经元凋亡，采用免疫印迹检测Akt和Bad(ser136)的磷酸化。

Methods Transient (15min) brain ischemia was induced by the four-vessel occlusion in Sprague Dawleyrats. The antisense oligodeoxynucleotides of Src and Fyn were used to suppress the expression of Src and Fyn by intracerebroventricular infusion once per day for 3 days before ischemia. After 5 days reperfusion, rats were perfusion-fixed with paraformaldehyde and cresyl violet staining were used to examine the survival of CA1 pyramidal cells of hippocampus.

采用Pulsinelli-Brierley 4动脉阻塞(4-VO)大鼠全脑缺血模型，缺血前连续3d脑室注射Src、Fyn反义寡核苷酸(antisense oligodeoxy-nucletides， AS ODNs)抑制Src、Fyn蛋白的表达后缺血，复灌5d，石蜡切片，焦油紫染色，图像分析测定单位面积内焦油紫染色细胞面积总和，与缺血组及错义寡核苷酸组(missense oligodeoxy-nucletides， MS ODNs)比较进行形态学分析。

Methods The staining status was compared of MMP 2, MMP 9, TIMP 2 and TIMP 1 in cryostat sections of tumor tissue in 35 NB patients by immunohistochemistry.

应用免疫组化SABC法检测 35例神经母细胞瘤标本中MMP 2、MMP 9及TIMP 2、TIMP 1的表达情况，并比较其在无转移、局部转移及远处转移患者肿瘤组织中的表达程度。

Methods Normal adult SD rats were perfused and cut into sections on a cryostat. Immunohistochemistry, immunofluorescent and Nissl staining technique were used.

正常成年SD大鼠5只，常规固定，恒冷箱切片，免疫组织化学、免疫荧光染色、尼氏染色。

Cytotoxicity of TNF in immortalized lymphoblastoid cell lines was determined by crystal violet staining.

应用结晶紫染色法检测淋巴母细胞系分泌TNF的细胞毒活性。

METHODS: Cytotoxicity of the combination of TNF-α and IFN-γ on HCC in vitro was measured by using a crystal violet staining method.

采用结晶紫染色法测定肿瘤坏死因子-α、干扰素-γ和两者联合用药体外杀灭肝癌细胞的能力。

Methods: The injury of rat aortic vascular endothelial cells was determined by the lactic dehydrogenase release and the proliferation of rat aortic vascular smooth muscle cells by crystal violet staining.

目的：研究蛋白激酶C抑制剂对纤维蛋白原降解产物引起的大鼠主动脉血管内皮细胞损伤的保护作用和平滑肌细胞增殖的抑制作用。

LDH release assay was improved and was compared with the crystal violet staining and the MTT colorimetry on sensitivity, repeatability and relateness.

对LDH 释放法进行改进，与结晶紫染色法和MTT比色法进行灵敏度、重复性和相关性比较。

For the cyclic intensified training group, the content of hydroxyproline in the Achilles tendons, which was taken as the amount of total collagen, was measured by alkaline hydrolysis, and immunohistochemistry staining was employed to analyze semi-quantitatively the dynamic changes in collagen Ⅲ expression.

结果 在训练过程中，跟腱的组织结构出现由以损伤为主到修复重建占主导的动态变化，循环训练模式下损伤表现出现早，程度轻，持续时间最短。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。