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The expression of AtPP2like gene(lec17 and lec26)in Cucumis melo determined by RT-PCR and the callose deposition in phloem measured by aniline blue staining were both induced by HrpNEa.

利用反转录PCR的方法，检测了甜瓜两个AtPP2类似蛋白的编码基因lec17和lec26，受到HrpNEa的诱导表达增强，同时通过苯胺蓝染色鉴定了胼胝质在HrpNEa处理的甜瓜植株中沉积增加。

The expression of AtPP2like gene (lec17 and lec26) in Cucumis melo determined by RT-PCR and the callose deposition in phloem measured by aniline blue staining were both induced by HrpN.

利用反转录PCR的方法，检测了甜瓜两个AtPP2类似蛋白的编码基因lec17和lec26，受到HrpN的诱导表达增强，同时通过苯胺蓝染色鉴定了胼胝质在HrpN处理的甜瓜植株中沉积增加。

Staining with the improved carbol fuchsin-aniline blue fluorescence method is a convenient and efficient way to observe the behavior of the callose wall of microspore mother cells during meiosis.

利用改良苯酚品红－苯胺蓝压片法，观察小孢子母细胞减数分裂过程中胼胝质的动态变化。

Callose staining with particular fluorescence dye-Aniline blue indic-ated that the degradation of callose was delayed.

利用胼胝质特异性荧光染料甲基蓝对花药进行染色，表明突变体胼胝质降解延迟，导致小孢子不能从四分体中释放出来，最终没有花粉粒的形成。

Seeds were sprouted at 25 ℃.The root-tip with 0.5~1.0 cm length were cut and treated in 4 ℃ refrigerator for 24 hours, fixed for 1~1.5 hours with Carnoy's fixative, differentiated for 10-15 min with hydrochloric acid and Ethanol 95 %(1:1), pressed to section in carmine for staining. Them were observed under microscope, then the samples that differentiated well chosen, covered on frozen station with mountin Canada balsam, for micro-photographing, counting and measuring.

方法］种子25℃萌发，待根尖长0.5~1.0 cm，冰箱4℃冷藏预处理24h，卡诺固定液固定1~1.5h，1：1的浓盐酸和95%乙醇解离10~15min，改良的石碳酸品红液染色，镜检取分散良好的标本，冰冻揭盖片，中性树胶封片显微照像、计数、测量。

Sclerostin expression and distances of each osteocyte to the canal surface and cement line were assessed for all osteonal osteocytes in 636 unremodelled osteons chosen from fields ( approximately 0.5mm diameter) with at least one canal staining for alkaline phosphatase (ALP; a marker of bone formation).

我们评估了对636个未重塑的骨单位中所有的骨单位骨细胞的硬化蛋白表达和每个骨细胞到管道表面和粘合线的距离，这些骨单位从至少一个管道碱性磷酸酶(ALP；骨形成标志物)染色的区域选择（直径大约0.5mm）。

Analysis of ovine sperm capacitation was assessed by different sperm staining types with CTC.The results showed that the percentage of capacitated sheep sperms in each treatment group ...

结果显示，在240 m in和360 m in时，各实验组中获能精子的百分数均明显高于对照组的(P.05)，说明一定浓度的孕酮有利于绵羊精子的体外获能。

Grenache were examined with Carbolfuchsin staining method and the length of chromosomes were measured with UNICHRO package.

Cre nache花药再生植株的染色体，并用UNICHRO软件对其数目及长度进行了测量。

Abstract］ Objective To investigated the cellular distribution and quantitatively of CEA in cerebrospinal fluid meningeal carcinomatosis cells by immunofluorescence staining and laser scanning confocal microscopy, explore the diagnostic value of the examination in the patients with meningeal carcinomatosis.

目的 联合应用免疫荧光细胞化学技术和激光扫描共聚焦显微镜技术检测脑脊液中脑膜癌细胞CEA的表达部位并定量测定其荧光含量，探讨该技术在脑膜癌病诊断中的价值。

Results The experiment of immunohistochemical double-staining showed there were cardinal red granules in violet cytoplasm of VEGF-C positive TAM, and VEGF-C was weakly positive in intratumoral tissue, and strongly positive in peritumoral tissue.

结果 免疫组织化学双重染色显示：VEGF-C阳性表达的TAM中可见在紫黑色胞浆内有深红色颗粒存在，且VEGF-C在肿瘤内呈弱阳性表达，而在肿瘤周围间质的TAM中表达呈强阳性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。