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After calcium induction, the alizarin red staining was performed to test the formation of calcium concentration.

并分别采用茜素红钙盐染色和油红O染色进行鉴定。

Alizarin red staining results showed that the calcified nodules were produced after induction.

茜素红染色结果表明,诱导后出现钙化结节。

Moreover, alkaline phosphatase, von-Kossa and Alizarin Red S staining on mineralized nodule formation were also performed.

结果显示,在RWHFMB内动态培养的成骨细胞生长良好,分泌大量的细胞外基质:中空纤维膜亦表现出良好的生物相容性。

MAIN OUTCOME MEASURES: Preparation of fibroblast feeder layer; growth of embryonic stem cells; results of alkaline phosphatase staining; cell differentiation tendency.

主要观察指标:成纤维细胞饲养层的制备情况,胚胎干细胞的生长状态、碱性磷酸酶染色结果及分化趋势。

Alkaline phosphatase staining: ES cell clones were stained dark blue, showing alkaline phosphatase activity.

碱性磷酸酶染色:ES细胞克隆被染成深蓝色,说明细胞显示碱性磷酸酶活性。

METHODS The changes of the proliferation and adhesion of the alkannin to the cells were measured by methyl thiazolyl tetrazolium.The effect of inducing apoptosis of the alkannin to the cells were observed by the flow cytometry,Annexin-V-FITC staining and transmission electron microscope.

方法应用MTT比色法检测新疆紫草素对细胞的增殖、黏附抑制作用,用流式细胞术、Annexin-V-FITC联合法及透射电镜检测新疆紫草素对胃癌细胞的凋亡诱导效应。

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株,经筛选后倒置荧光显微镜观察细胞形态及荧光状态,挑取荧光最强的克隆,命名为ES-GFP细胞,进一步扩增培养,观察细胞生长及荧光表达情况,行细胞分化全能性鉴定包括碱性磷酸酶(alkine Phosphtase,AKP)染色、体外胚胎体(embryonic body,EB)形成实验及裸鼠体内成瘤实验。2。

HE and Masson staining were used to determine the extent of alveolus inflammation and pulmonary fibrosis respectively.

结果 各时间点治疗组大鼠PaO2均随治疗剂量增大逐渐升高,同一组内则随时间延长逐渐升高;肺组织中TGF-β1和CTGF的表达量随时间延长逐渐减少。

Immunohistochemical staining during different stages in ameloblast odontoblast and enamel free area cells were measured by the image analysis,the date of which was handled with SPSS11.5 software.

随后做图像分析测定不同发育阶段的成釉细胞、成牙本质细胞、无釉区细胞的免疫表达水平,所得数据用SPSS11.5 软件进行统计分析。

Methods We used retrograde CTb immunocytochemical and NADPH-d histochemical staining methods after microinjecting CTb into cortical amygdaloid.

采用霍乱毒素B亚单位逆行追踪和还原型尼克酰胺腺嘌呤二核苷酸脱氢酶组织化学双重染色相结合的方法。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。