查询词典 staining

Cell intensity included: 0 was achromatic color, 100 was weak staining, 200 was moderate staining, 300 was strong staining.

细胞染色强度分为：0分为无色，100分为弱染色，200分为中等染色，300分为强染色。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Decalcification solution has no effect on the staining of mast cell in nasal mucus by histochemical staining and immunohistochemical staining.

不同脱钙液（8%盐酸脱钙液、EDTA脱钙液、混合酸脱钙液）处理不影响鼻腔粘膜中肥大细胞的组织化学和免疫组织化学染色。

The present studies showed that two cell populations were found in haemocytes: large cell with high granularity and small cell with low granularity by flow cytometry FCM on light scanttering pattern. Two distinct cell types were identified based on phase contrast microscope: one type of cell was dark and dioptric aberration, while the other was bright and dioptric strong. By Giemsa and H.E staining, cytoplasmic staining were heterogeneous and internal particles were obvious in one type of cell, while cytoplastic staining were homogeneous and internal particles were inexistent in the other type of cell. By transmission electron microscope, we found that the mitochondria, Golgi apparatus organelles were rich and internal particles were obvious in one type of cells, and contrary to the another cells.

流式细胞术光散射图谱显示血细胞被分两类，一类为颗粒度高的大细胞，另外一类为颗粒度低的小细胞；相差显微镜观察显示，血细胞可分为胞体暗、折光性差和胞体明亮、折光性强的两类； Giemsa和H.E染色显示细胞分为胞质染色不均一、胞内颗粒明显和胞质染色均一、胞内颗粒不明显的两类；透射电镜超薄切片观察显示，颗粒明显的细胞胞质内线粒体、高尔基体等细胞器较丰富，颗粒不明显的细胞胞质内细胞器较少；负染结果表明血细胞主要分为表面不光滑、突起明显和细胞表面光滑、突起较不明显的两类。

Marrow smears were routinely stained with wright staining and iron staining, while sections of biopsy specimens embedded in plastic were stained with hematoxylin-giemsa-acid fuchsine and gomori reticular fiber staining.

骨髓涂片常规做瑞氏染色和铁染色，骨髓活检切片做苏木精－姬姆萨－酸性品红染色和gomori网状纤维染色。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Immunohistochemical staining indicated that Nattokinase positive staining grains were found in the brush-border membrane of epithelial cell from duodenum, jejunal to ileal in the Nattokinase extract group, and they showed most predominant in the jejunum, and there were many nut-brown positive staining grains in kytoplasm of epithelial cell.

利用链霉菌抗生物素蛋白-生物素-过氧化物酶复合物(Streptavidin-biotin-peroxidase complex，SABC)免疫组织化学技术，在国内外，首次对兔口服摄入的纳豆激酶进行肠道吸收定位研究。

All 31 YSTs (5 pediatric and 26 postpubertal) showed strong positive SALL4 staining in more than 90% tumor cells but had negative OCT4 staining. Both spermatocytic seminomas showed positive SALL4 staining in 80% to 95% tumor cells in all 3 types of tumor cells with weak-to-moderate staining intensity.

为了检验SALL4的特异性，我们还给23例睾丸的非GCTs（10例间质细胞瘤、4例支持细胞瘤、3例腺瘤样瘤、3例睾丸旁横纹肌肉瘤、2例弥漫性大B细胞瘤、1例睾丸网的乳头状囊腺瘤）和275例非睾丸性肿瘤（158例转移癌、12例转移性黑色素瘤、11例原发性和2例转移性间皮瘤、72例原发性和20例转移性肉瘤）做了SALL4免疫染色。

Four weeks later, the vein grafts were removed ,and HE staining, P/VB staining ,Ⅷ R:Ag staining, and Oil Red "O" staining were performed. Establish hypercholesterolemic rabbit model of vein grafting with PTFE external stent on one side and unexternal stent on the other side.

建立兔的高胆固醇血症模型并在其基础上行动静脉吻合，定期测量兔的血清总胆固醇(TC，4周后行VG的HE染色、VB染色、Ⅷ因子染色、油红&O&染色。

For theimmunohistochemical double staining of MBP/NF,S-P method was used for bothstaining,with brown-yellow and purple-blue DAB for the first time and second timestaining.For the immunohistochemical double staining of CD31/IgM andGFAP/Ki-67,the first time staining was performed by S-P method with brown-yellow DAB,while the second time staining was performed by SABC-AP methodwith NBT/BCIP.

双重免疫组化染色包括：MBP/NF，两次染色均用S-P法，第一次显色为棕黄色DAB，第二次显色为紫蓝色DAB，以及CD31/IgM和GFAP/Ki-67，第一次染色用S-P法，棕黄色DAB显色，第二次染色用SABC-AP法，NBT/BCIP显色。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。