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Bronchoalveolar lavage and wright staining analysis showed that thetotal number of the all inflammatory cells in asthma model group andthe normal control were (36.2±4.6)×10~6/ml and (9.5±1.6)×10~6/ml.There was significant difference between the two groups (p<0.01).Meanwhile, the number of acidophilic cell, macrophage andmacrophage were also increased in asthma model group rather thanneutrophilic granulocyte.

支气管肺泡灌洗和细胞记数显示,哮喘模型组支气管肺泡灌洗液细胞总数为(36.2±4.6)×10~6/ml,与正常对照组(9.5±1.6)×10~6/ml,相比显著增加(p<0.01),同时嗜酸性细胞、巨噬细胞和淋巴细胞增加明显,而中性粒细胞变化不明显。

The mice were randomly divided into two groups ( n =24 per group): the experimental group, the cultured cells were injected into the mice through the tail vein; the control group, the equivalent serum-free medium was injected. Six mice from each group were killed at 7, 14, 21, and 28 days after operation to receive HE staining, PCR gel electrophoresis, immunohistochemistry staining, and hepatic function detection.

将小鼠随机分为实验组和对照组( n =24),实验组将诱导后细胞(1.6 × 105 个/只,含CD34+细胞1× 104 个)通过尾静脉注射入肝损伤小鼠体内,对照组注射等量无血清培养基,术后7、14、21、28 d 两组分别处死6 只小鼠,行HE 染色、PCR 凝胶电泳、免疫组织化学染色及肝功能检测。

The results indicated that the techniques of afforestation in plots,container seedlings afforestating and staining roots with phosphate fertilizer haveimproved afforestation survival ratio,height growth and diameter growth of young Pinus elliottii,and Choerospondias axillaris have also been improved by the techniques of staining roots with ABT and cutting stem.

结果表明,有利于提高湿地松造林成活率和促进幼树树高、胸径生长的技术措施分别是集团造林、选用容器苗及磷肥蘸根;而有利于提高南酸枣造林成活率和促进其幼树树高、胸径生长的技术措施为截干和 ABT生根粉沾根处理。

According to Anthony Kiedis:"Purple stain refers to a double entendre of a girl's hair dye staining your pillow and a girl's menstrual flow staining your everything."

根据Anthony的说法,Purple Stain拥有双重含义。一是指女孩儿的染发剂弄脏你的枕头,二是指女孩儿的月经弄脏你的一切。

Methods The rat thoracic duct endothelia were cultured and the morphology observed and identified by TEM and Factor Ⅷ-related antigen immunohistochemically. Wistar rats were used to make the animal model of primary colorectal cancer, the cancer type were appraised by HE staining and PAS Reaction. The expression of VEGF-C and its receptor Flt-4 and ICAM-1 in the cancer tissues were carried through immunohistochemistry staining and In situ hybridization.

通过体外培养大鼠胸导管内皮细胞和构建大鼠原发性大肠癌动物模型,观察内皮细胞的形态和生长状态,用透射电镜和Ⅷ因子相关抗原进行鉴定,用HE染色和过碘酸雪夫氏反应区别癌组织的类型和期别,用免疫组织化学方法和原位杂交方法研究内皮细胞和大肠癌组织的VEGF-C及其受体Flt-4和ICAM-1的表达。

Ocular crosssections were prepared for staining of HE and eyeball slicing parallel TUNEL staining. To observe the cell morphology of 4 groups RGCL.

眼球切片并行TUNEL染色和HE染色,观察4组RGCL的细胞形态,免疫组化染色观察诱生型一氧化氮合酶的表达。

The wound healing of all the animals were observed with healing intensity, liquid scintillation counter, AgNORs staining, VG staining and transmission electron microscopy.

利用家兔自体穿透性角膜移植模型,采用测量伤口愈合强度、液闪计数、AgNORs染色、VG染色和电镜等方法,观察家兔PKP术后伤口愈合情况。

Nucleus changes in apoptotic cells were investigated by using Hoechst 33258 staining and photofluorography. Apoptotic rate was evaluated by propidium iodide staining and flow cytometer.

应用Hoechst 33258染色法观察凋亡细胞的形态学改变;PI染色流式细胞仪检测细胞凋亡率;Western印迹法检测Bcl-2蛋白的表达。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶染色流式细胞技术检测细胞凋亡率;Hoechst33258染色检测细胞凋亡的形态学变化;罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位;2',7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

The result of immunohistochemical staining was different from that of conventional Congo red Staining.

βA4在脑组织中的表达并非Alzheimer病所特有,而是多种神经变性疾病的共同特征;βA4免疫组化法的阳性结果不同于传统的刚果红染色法。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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