查询词典 staining

With infection progress, the high intensity of staining was observed in the perinuclear regions and membrane area at 48hr and 72hr p.i., and the staining intensity showed high co-localization with DV2 E protein.

感染后24hr荧光的强度无明显变化，随着感染时间的延长，整合素β3抗原的荧光强度逐渐增强，在感染后48hr和72hr，细胞均呈现较强的荧光。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

During the surgery, the lesion was found to be a solid tumor. A surgical wedge biopsy was done and purulent dark yellow fluid was drained from the center of the mass. Histologically, there was a solid pattern of rhabdoid and anaplastic round or oval cells haphazardly arranged with poor differentiation. Immunohistochemical staining was positive for vimentin, a mesenchymal marker; the reticulin staining was consistent with carcinoma.

我们实施了手术切片及肿瘤中心脓状物引流，病理组织学上，我们发现了不规则或未分化的横纹状、圆形及椭圆形细胞；免疫组织染色上，细胞广泛的对非上皮标记vimentin呈阳性反应、且其reticulin染色呈现出腺癌的形式；肉瘤及腺癌的特色皆可在切片中看到，所以我们的诊断是类肉瘤性线癌。

Immunohistochemical assay were performed with HGV NS5 McAb. Results One of four samples showed positive staining, some of which located in cytoplasm and some of which in cytonucleus and cytomembrane. The number of positive staining cells are not more. More of them disrtibuted scatteredly, some of them located.

结果 1例呈阳性染色，光镜观察显示：特异染色大部分定位于肝细胞胞浆中，部分有核着色和膜型染色，呈黄色或棕黄色颗粒状，阳性细胞数量少，大多散在分布，部分呈灶性分布；阳性细胞周围可见较多淋巴细胞浸润。

At postnatal 1st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining. NGF positive staining in the testes was observed in interstitial cells, spermatogenetic cells, sustentacular cells and Leydig cells at 3rd week. After the postnatal 5th week, NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells, but the intensity of reaction was weaker than that at 1st and 3rd weeks.

免疫组化定位分析显示：睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内，1周龄睾丸组织免疫阳性反应主要位于支持细胞，精原细胞也有着色；3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性；5周后的睾丸组织内神经生长因子呈低水平表达，主要表达于间质细胞和生精细胞内。

The depth of tectal layerⅠand the morphological characteristics of tectal cells in layerⅠwas investigated using Nissle staining, Golgi staining, and postmortem labeled with DiImethod. Most ofⅠcells appeared shuttle, others showed round, oval, pyramidal, triangular,and polygonal etc. The lateral region of layerⅠwas thicker than the ventral and the dorsalregions.

本研究使用Golgi-Cox法，灌流固定后尼氏体染色、羰花青荧光染料DiI(1，1′-dioctadecyl-3，3，3′，3′-tetramethylindocarbo-cyanine perchlorate)逆向神经标记技术，对雏鸡视顶盖Ⅰ层的厚度和该层的细胞形态、大小进行了观察研究和数理统计，并对不同日龄鸡胚的Ⅰ层厚度进行了数理统计及分析研究。

The osteogenesis was observed by HE staining, modified Mallory's trichrome staining, and image analysis.

随着生物材料、细胞生物学的发展，使应用组织工程技术修复组织缺损成为可能。

Methods Pathological sections of aorta were performed with hematoxylin-eosin staining and Masson＇s trichrome staining.

目的探讨睾酮和氟他胺对动脉粥样硬化斑块稳定性与血清炎症因子的调节作用。

Specific stainings includ silver staining,Nissl bodystaining and modified trichrome staining with improvements.

组化染色包括嗜银染色、尼氏体染色及新的改良三色法染色。

Methods: Fresh stools of the patients suffered from diarrhea were examined with direct wet-mount, iodine staining and trichrome staining.

收集腹泻患者当日新鲜大便，用生理盐水直接涂片、碘液染色及三色酸染色法染色镜检。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。