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3Chondrogenesis was assessed using Alcian blue staining, Toluidine bluestaining, Safranin O/Fast Green staining at 7 and 14 days, still collagenⅡimmunohistochemistry and aggrecan immunofluorescence at 4, 7 and14 days after initial chondrogenic induction of adipose -derivedmesenchymal stem cells.

3观察脂肪间充质干细胞在诱导后的生长增殖情况、形态变化,于诱导7、14天后应用阿尔新兰染色、甲苯胺兰染色、藩红0/固绿染色,以及4、7、14天后应用Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光评价其成软骨能力。

More thicker epiphyseal plate were found in the left femurs than the right femurs of the group fixed with plates at 3 and 6 month after operation. In the group fixed with plates, Safranine O staining showed epiphyseal plates ot the left femurs had more fuscous staining than the right femurs at 3 and 6 month after operation. Electron microscopy also found that the cells of the epiphyseal plates of left femurs were more eugenic than the right femurs in the group fixed with plates at 3 and 6 month after operation.

光镜学实验结果示滑动钢板固定的左侧股骨骨骺板总厚度及肥大细胞计数均明显大于普通组;组织化学观察示滑动钢板固定的左侧股骨骺板于术后3个月及6个月Safranine O组织化学染色明显深于普通组;电镜观察示滑动钢板固定的左侧股骨骨骺于术后3个月及6个月软骨增殖区软骨细胞分裂及生长较普通钢板固定的右侧活跃。

Results: Average protein spots were 426 in the diarrhoea-predominant IBS group by using Coomassie brilliant blue staining, while the constipation-dominant IBS group by using silver staining got average 581 protein sptos.

结果:考马斯亮蓝法染色用于腹泻型IBS患者,平均得到蛋白质点426个;硝酸银染色法用于便秘型IBS患者,平均得到蛋白点581个。

Liver tissue inflammation and collagen deposition were observed with HE staining and sirus red staining;Liver function,Liver tissue hydroxyproline content were determined biochemically.Results:Compared to that of normal rats,The serum ALT、AST activity of model group elevates significantly,Alb and TP lower markedly.

观察模型大鼠体重、肝脾体重等变化,HE染色及天狼猩红染色观察肝组织炎症与胶原沉积病理改变,检测血清肝功能及肝组织羟脯氨酸(Hydroxyproline,Hyp)含量。

To investigate the distribution pattern of mesenchymal stem cells in human placentas by immunofluorescence staining and immunohistochemical staining.

目的探索间充质干细胞在人胎盘组织中的分布规律。

The penetration of MPS becomes apparent by the pronounced metachromatic staining in corium and subcutis; i.e. cells, fibres and ground substance show an intense reddish-violet staining due to the deposition of MPS.

真皮和皮下组织的染色显示MPS制剂具有显著的渗透性;细胞、纤维组织和基质因MPS的沉淀而呈紫红色。

CASE REPORTSThe examination including slitlamp examination, tear breakup time, schirmer I test and fluorescent staining were performed in 97 outpatients with symptoms of asthenopia, foreign body sensation and dryness. The positive cases in BUT, SIt, fl staining and obstruction of tarsal glands and were 51,38,31 and 34 cases, respectively.

病例报告]对97例具有视疲劳、异物感及眼干涩等症状的门诊患者进行裂隙灯检查、泪膜破裂时间实验、泪液分泌实验及角膜荧光染色等检查,所有患者中泪膜破裂时间实验、泪液分泌实验、角膜荧光染色及睑板腺异常的阳性例数分别为51,38,31,34例。

At day 7, 14 and 21 after induction respectively, Toluidine blue staining and immunocytochemical staining were performed to detect differentiation.

在诱导第7,14,21天分别行甲苯胺蓝染色、免疫细胞化学染色检测其分化结果。

The result was observed 6 weeks later by HE staining,toluidine blue staining and type Ⅱ collagen immunohistochemistry.

2周后植入自体股部肌袋,6周取材,HE、甲苯胺蓝染色、Ⅱ型胶原免疫组化鉴定诱导分化效果。

RESULTS: The levels of toxicity ranged from 0 to 1 grade. HE staining and toluidine blue staining demonstrated that PDLCs could adhere and proliferate on the surface of SIS in good condition.

结果:MTT法检测证实细胞毒性为0~1级,材料无细胞毒性;HE染色和甲苯胺蓝染色可见PDLCs在SIS表面和内部均生长良好,显示SIS具备良好的生物相容性。

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