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Methods The AGS cellseeded coverslips were prepared, then the two different status of Lactobacillus acidophilus and two different forms of Hp were added in different order. 2 hours later, the bacteria were Garm's stained, and the adherence of Hp to AGS cell were observed by microscope and the adherence index was calculated.

制备人胃上皮腺癌细胞系细胞爬片,将两种状态嗜酸乳杆菌和不同形态Hp菌分组按不同顺序加入,作用2 h,革兰染色后光镜观察分析Hp黏附指数产生的变化,荧光抗体染色法定性评价黏附于AGS细胞的Hp密度的改变。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记,对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml),以不同扫描序列T1WI,T2WI,T2*WI(GRE进行MR成像,再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像,并测量信号强度,进行统计学分析。3缺血再灌注肾损伤模型建立和处理,然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只:注入标记细胞者10只,注入未标记细胞者6只),两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照,然后对收集的信号强度进行统计学分析。

Methods Choose healthy,adult Wistar rats for disposable injection of SIZ in abdomenal cavity to induce diabetes.Retinal vascular nets and eyecups were stained for observation under lens.

方法选择健康成年 Wistar 大鼠一次性腹腔注射链脲佐菌素诱发大鼠糖尿病,将视网膜血管网和眼杯进行染色后,镜下观察。

Aside from this, Dale Tiffany also presents various items in their product line, cheap dvds , including products that make use of stained glass, reverse-painted glass, Favrile hand blown glass, and many other techniques.

除此之外,也呈现出各种物品蒂尔在其产品线,廉价dvd,包括产品,利用彩色玻璃制成,reverse-painted玻璃吹制玻璃手,Favrile,和许多其他的技巧。

Immunohistochemistry were positive for CD34 in some region where the fibroblast actively proliferated, whereas fibrocytes in mature fibrous tissue were negatively stained.

CD34免疫组织化学染色显示一些增生活跃的纤维母细胞明显表达CD34,成熟的纤维组织内的纤维细胞CD34则为阴性。

To his roots, such as silver,not stained; its leaves, lush and vivid green; its flower like Calendula Units silver, elegant×, snakes, fine-featured beauty, white and sweet, fresh fragrance and a long flowering period.

其根,如银丝,纤尘不染;其叶,碧绿葱翠传神;其花,有如金盏银台,高雅绝俗,婀娜多姿,清秀美丽,洁白可爱,清香馥郁,且花期长。

Nobody can quite remember what kind it is, but the red fruit has flesh that tastes like strawberries and is stained with a blush of pink below the skin.

谁也不能完全记住它是什么样,但有肉红色的果实,像草莓口味,是与以下的皮肤粉红色腮红染色。

Unfortunately, in the end the painter flapped the brush and the beautiful mirror floor was stained with the paint.

不幸的是,完工的时候,油漆工拍打刷子,美丽光亮的地板被油漆玷污了。

Methods Hepatic carcinoma cell lines of high and low potentialities of lymphatic metastasis were injected into the footpads of Balb/c mice. Their metastases to lymph nodes were examined. The tumor tissues of each group were stained with 5′-nucleotidase-ALP to observe the lymphoangiogenesis.

将淋巴道高、低转移潜能的肝癌细胞接种于Balb/c小鼠,观察成瘤及转移情况;对肿瘤组织进行淋巴管染色,观察淋巴管生成情况。

This paper focuses on discussing mosaic icon paintings in the Byzantine period, icon frescos in the Romanesque period and Stained Glass icon paintings in the Gothic style period.

本论文主要针对拜占庭时期的马赛克圣像画、罗马式时期的圣像壁画、哥特式时期的彩色玻璃圣像画进行论述。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。