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Describing index: Before the interventional therapy,the number of femoral head 's blood vessel wasslender and the number of ramus anastomoticus was rare.The blood vessel was stegnotic and even was interrupted .The stained range was localized and dark . After the interventional therapy,the embolic blood vessel became easy and smooth . The blood vessel was thick and excurrent .The stained range was wide and light-coloured.

描述性指标:介入治疗前,股骨头供血血管稀少,吻合支少,血管狭窄、中断,对比剂染色范围小,颜色深;介入治疗后闭塞中断和/或严重狭窄的血管再通,髋部血管数量增多,血管径增粗,血管延伸,对比剂染色范围变大,颜色浅。2。

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry.

肝脏对血流的廓清率达1800ml/min,尤其结直肠静脉血主要经门静脉流入肝脏,因此在结直肠癌血道转移中,肝脏是最常见的靶器官。。

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry. The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.

取健康的猪甲状腺细胞培养24h后,加入不同浓度的碘化钾,观察细胞的形态改变;琼脂糖凝胶电泳、流式细胞术分析细胞DNA的降解;MDA法测定细胞膜的脂质过氧化程度。

Methods: Combined with the morphological character of the gangliocyte in the intestinal wall under microscope, the stained result and distributed character of the diseased intestinal segment in clinical and pathological diagnosed as congenital macrocolon was stained and compared with S-100 and NSF and with the immunohistochemistrical technique.

结合光镜下肠壁神经节细胞的形态特征,并应用免疫组化技术对25例临床及病理诊断为先天性巨结肠的病变肠段行S-100和NSE染色,比较染色结果和分布特点。

Observation of cultured cells stained by haematoxylin and eosin: It was shown that the cells presented round or ellipse. There was little cytoplasm, presenting acidophily. Nucleus was big and round, single in the center, deep stained.

培养细胞苏木精-伊红染色观察结果:可见细胞圆形或椭圆形,胞质少呈嗜酸性,细胞核大而圆,单个居中,着色深。

Meanwhile, in the CA3 area of hippocampus in NS group, a small amount of N-Cadherin positive cells were visible and were slightly stained. Compared with NS group, in PTZ seizure group, N-Cadherin positive cells had a marked increase in area CA3 and dentate hilus (P.01), but they were strongly inhibited in PDTC group (P.05). There was no correlation between the expressions of N-cadherin, Timm-stained granules and between the age of rats with seizure.

同时,NS组14天和28天大鼠海马CA3区可见少量的N-Cadherin阳性细胞,着色不深;PTZ致惊组海马CA3和齿状回门区的N-Cadherin阳性细胞与NS组相比明显增多(P<0.01),PDTC预处理后相同区域内N-Cadherin阳性细胞较PTZ致惊组明显减少(P<0.05)。N-Cadherin与Timm染色颗粒的表达结果与惊厥鼠的日龄并无关联性。

RESULTS: HE stained cells were fusiform-shaped with pseudopodia, round or oval nucleus. The cytoplasm was blue after stained with the toluidine blue. The immunohistochemical test revealed that there existed positive expression of type Ⅰ collagen in the cells. In the presence of 10% calf serum, IGF-Ⅰ significantly improved cell proliferative activity in a dose-dependent manner within effective dose range.

结果:苏木精-伊红染色细胞多为梭形,有伪足伸出,细胞核为圆形或椭圆形;甲苯胺蓝染色,胞浆为深蓝色;免疫细胞学方法检测表明纤维环细胞有Ⅰ型胶原表达;在体积分数为0.1的血清条件下,胰岛素样生长因子Ⅰ能提高细胞的增殖活性,并且在有效浓度范围内呈剂量效应关系。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400

Most of the cells couldn't be stained by trypan blue,and were TUNEL positively stained.

大部分细胞为台盼蓝拒染,并呈TUNEL阳性着色。

The infused and the contralateral, non-infused cochleae were harvested and stained with X-Gal, the Ad-LacZ infected cochlea were stained with blue color.

我们将Ad-lacZ病毒注入豚鼠内耳中,分别在病毒注入二天、一周、二周后取材固定进行X-Gal染色可观察到,整个耳蜗从蜗底到蜗尖均被染成兰色。

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