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Material and methods: 18 cases of keratoconus and 7 cases of normal cornea was stained by haematine-eosin, iron stain, alcian blue stain method and observed by transmit electron microscope and scanning electron microscope.

材料与方法：取角膜移植术钻下的圆锥角膜组织18例，无眼病的尸体角膜7例，经苏木精-伊红染色、铁染色、甲苯氨蓝染色、阿尔新蓝染色和透射电镜和扫描电镜观察。

Porcelain ware soaker HR--206 This product is a kind of soaking powder that can quickly remove coffee stain,tea stain,food residue and other color spots from dishware,it can be used to wash or soak dishware,such as plater,teacup,knife and fork,it can quickly remove stains without affecting brighteness of dishware,and it can be used in soft or hard water.

餐饮系列瓷器浸渍剂 HR----206 本品是一种粉状洗涤除渍浸泡剂，能迅速除去茶杯、盘碟上的咖啡渍、茶渍、食物残渣和其它色渍等。适用于洗涤或浸泡餐碟、盘子、刀叉、茶杯等餐具。

The stem was cut into several segments away from the pine seeding, arranged and collected in the order of chronology time and distance from where they were inoculated. To separate B.xylophilus coarsely out from the stems and made them into paraffin slices. And be observed under the potics microscope by dyed with safranin-fast green contrast-stain method and PI fluorescence stain method. In order to find out how about the nematode rigour distributing , but also ebb and flow of their amounts after invading the host pine seeding. Seek the pathological changing orderliness of host cells in different position along with the remotion of nematodes. By deeply analysing the variety of host cell nucleolus to estimate whether the host cell took place the programmed cell death after inoculated B.xylophilus.

按时间进程和与接种点上下距离的远近，对松苗分茎段进行粗分离和制作成石蜡切片，分别用番红-固绿双重染色法和碘化丙锭荧光染色法在光学显微镜下观察，以了解线虫侵染后在寄主体内的准确分布和数量的消长，寻找随着线虫的移动扩散，寄主不同部位细胞病变的数量、速度发生的变化规律，并进一步通过分析其细胞核的变化来判定松材线虫侵染后是否能诱发寄主细胞发生程序性死亡(programmed cell death，PCD)。

Safranin In microscopy, a permanent red stain used to stain nuclei in plant cells.

藏红：一种在显微镜下观测标本所使用的植物染料，可使植物细胞的核结构被永久浸染为红色。

muscles biopsy of 25 patients with lipid storage myopathy were freezed and cut into slices. Specimens by routine histology and acid or alkaly myosin ATPase, oil red O and PAS stain were observed the difference of types of keletal muscle fibers by ATPase stain and degree of fatty and glycogen dispersed in sarcoplasm,explored substancs metabolite .

方法将25例LSM患者活检骨骼肌作为研究材料，做冰冻切片，采用常规组织学和酸、碱预孵育肌球蛋白ATPase法、油红O法、PAS法观察各型肌纤维ATPase染色差别及肌浆内糖原和脂肪的分布，以探讨其物质代谢。

The rat FC model was developed by warm water immersion (45℃). The memory capacity was tested by using a Morris water maze method. Neuronal changes were detected by thionine stain, TUNEL stain and electron microscopy.

采用Morris水迷宫方法检测大鼠的空间学习记忆能力；采用尼氏染色、TUNEL染色和电镜方法观察神经元损伤情况。

Results Positive rate of Amsel was 28%, one step sialidase was 34%, Gram-stain was 30% and amines was 24%. Using Amsel as criterion, the sensitivity of one step sialidase and amines was 92.9% and 71.4%; specificity was 88.9% and 94.4%; positive predictive value was 76.5% and 83.3% negative predictive value was 97% and 89.5%, using Gram-stain method as criterion, the sensitivity of one step sialidase and amines was 93.8% and 66.7%, specificity was 92.1% and 94.3%, positive predictive value was 83.3% and 83.3%, negative predictive value was 97.2% and 86.8% respectively.

Amsel法阳性率28%，组织多胺试验阳性率24%，一步法唾液酸酶活性的检测试验阳性率34%，革兰染色细菌评分法阳性率30%；唾液酸酶活性检测法与Amsel法，革兰染色细菌评分法相比较差异无显著性；组织多胺试验与Amsel法，革兰染色细菌评分法相比较差异无显著性；以Amsel法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为92.9%和71.4%，特异性分别为88.9%和94.4%，阳性预期值分别为76.5%和83.3%，阴性预期值分别为97.0%和89.5%；以革兰染色细菌评分法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为93.8%和66.7%，特异性分别为92.1%和94.3%，阳性预期值都为83.3%，阴性预期值分别为97.2%和86.8%。

Results The small regenerating fibers, central location of nucelus and basophilia endochylema were observed under HE stain both in the patients with DMD and PM. Anachromasis of antiNCAM monoclonal immunohistochemistry stain was found in the regenerating muscle fibers. Positive Ezrin expression was also detected in the regenerating fibers. However, this expression tapered gradually as the mature process of skeletal muscle. The expression of Ezrin was negative in the mature fibers.

结果 DMD、PM患者被检肌HE染色所见再生肌纤维直径较小、核位于中央、胞浆嗜碱性；NCAM染色再生肌纤维深染；再生肌纤维Ezrin呈阳性表达，伴随肌纤维成熟Ezrin表达逐渐减弱，成熟肌纤维无Ezrin表达；成肌细胞Ezrin呈阳性表达。

Methods Stool specimens of 4 048 students at different educational levels were collected, oocysts of cryptosporidium were identified with auramine - phenol stain and improved ami - acid stain.

方法采集安徽省各地市不同教育层次学生粪便标本共4048份，采用金胺-酚染色法和改良抗酸染色法检查隐孢子虫卵囊。

The cancer cells are in different differentiation periods: the chromatin of the young cancer cell's nucleus is rich in color and its cytoplasm is basophilous. The young cancer cells don't form into typical glandular cavity; The mature cancer cell is columnar or cubical and its nucleus is located at the base of the cell in gland tube-like arrangement; The decrepit cancer cell stain thin while its nucleus stain dense. The severer's nucleus disintegrate into small fragments. The decrepit cancer cell's arrangement is disorganized, only keeping its glandular shape.

显微镜下癌细胞呈现不同的分化程度：幼稚型癌细胞胞核染色质丰富，胞质嗜碱性，不形成典型腺腔；成熟型癌细胞呈柱状或立方状，细胞核位于细胞基部，呈腺管样排列；衰老型癌细胞胞桨染色变淡，胞核浓染，严重者胞核碎裂成细小碎片，衰老型癌细胞排列紊乱，仅保留腺体样结构轮廓。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。