查询词典 stain-resistant

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

An amount increase ofPAI-3 andK10 was observed after theKCswere cultured for48 h in high calcium medium,butat72 h the exprssion ofPAI-3 decreased. The positive stain ofPAI-3 wasmainly localized in perinuclearmembrane in lowcalcium medium and in cytoplasm in high calcium medium. PAI-3 was incorporated into cornified envelope ofterminally differentiated keratinocytes.

结果 高Ca2+浓度下培养24 h后，分化标记物K10弱阳性表达， PAI-3呈阳性表达；培养48 h后， K10强阳性表达，而PAI-3的阳性表达明显增加， 72 h后，表达开始降低，在低Ca2+浓度下阳性染色主要位于细胞核膜周围而高Ca2+浓度下主要位于细胞质，同时， PAI-3存在终末分化KCs角质壳中。

The right parietal cortex contusion was made with stab wound and the rat was killed at various survival time( 30min, Ih, 3h, 6h, 12h,ld, 3d, 7d, 14d, 21d). Paraffin sections(4 μ m) were cut coronally of the whole brain and stained with antibody of GFAP, the hemotoxylin eosin stain was done in the mean time.

实验组用针刺法造成大鼠右顶叶脑挫伤后，按30min，1h，3h，6h，12h，1d，3d，7d，14d，21d不同时间分批处死动物并立即取出全脑，同时颈静脉取血2ml，正常对照组不造成颅脑损伤，直接处死动物后立即取材。

In ACC, NGF,TrkA and p75 mainly existed in cells of duct-like, cribriform and mass-like structures.According to the optical density scanning of immunohistochemistric stain of NGF and TrkA in salivary gland and tumor tissues and quantification analysis, it was shown that the average optical density of NGF and TrkA increased in order of salivary gland tissues around neoplasm, mucoepidermiod carcinoma, and adenoid cystic carcinoma.

2叫 吉林大学硕士学位论文通过对NGF和TrkA在涎腺组织和涎腺肿瘤中的免疫组织化学染色结果进行光密度扫描，对所测平均光密度定量分析，结果显示：NGF和TrkA平均光密度值按照肿瘤周围涎腺组织、豁液表皮样癌和腺样囊性癌依次升高，统计学分析在各组间均有极显著差异(P.01)，表明NGF和TrkA通过自分泌机制调控涎腺腺样囊性癌和豁液表皮样癌的生长与增殖，通过旁分泌机制参与涎腺腺样囊性癌向神经周围的浸润。

Immunohistochemistry stain shows: A lot of positive express of NF-κB were found in the cytoblast and endochylema of operated group plantaris and the activity express of iNOS wasn't found in control.

3免疫组化结果显示：正常对照组骨骼肌细胞基本无NF-κB表达，实验组骨骼肌细胞可见胞核及胞质大量NF-κB表达。

N IND sections, the picture of dysplasia neurons can be distinguished from the normal easily with a smaller cell and cytoblast diameter, a distinct increase of number, less and stain thickened cytoplasm, nucleolus exit or not.

ND病变肠段粘膜下及肌间均有巨神经丛，丛内各成分细胞均呈阳性反应，神经丛内有大量大小不等细胞，为胶质细胞与发育不良神经元，其中可见部分细胞胞体较大。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

Destain:To remove stain from to aid in microscopic study.

使褪色：为有助于用显微镜进行研究而使褪色。

The tissue section of transbronchial lung biopsy specimens showed diffusedly infiltrated small lymphocytes and a lymphoepithelial lesion. CD20 was positive and CD3, CD5, CD10, CD21, CD23, bc12, bc16 were negative in immunohistochemical stain.

支气管镜下可见左肺下叶、右肺中叶支气管狭窄，用支气管镜进行透壁肺活检，病理为小淋巴细胞弥漫浸润，可见淋巴上皮病变；免疫组化CD20阳性，CD3，CD5，CD10，CD21，CD23，Bc12和Bc16阴性。

The paper analyses the effect of brake resistance of discal brake stop system,stain of brake_plate and the rellability of hydraulic station on elevator's safety running by abundantly spot testing.

通过大量的现场测试，分析了盘式闸制动系统的制动器阻力、闸盘污染以及液压站的可靠性对提升机安全运行的影响，提出了改造意

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。