查询词典 stage manner

At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达，tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常，心脏畸形随着注射剂量的增加而出现比较一致的表型，而c-MO组胚胎没有出现明显的畸形；tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度，验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性；tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等，并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

During each stage of angiogenesis, there are many different factors that take action synergically in temporal and spatial manner to the ongoing of vessel formation.

血管生成，即从原有血管生发出新的血管的过程，是体内最为基本和重要的过程，也是维持机体稳定性与完整性以及组织和器官功能的重要方式。

During each stage of angiogenesis, there are many different factors that take action synergically in temporal and spatial manner to the ongoing of vessel formation. Accorrding to their attribution to angiogenesis, these factors can be generally divided into positive and negative regulating factors.

在血管生成的各个阶段，有多种因子在时间和空间上协同作用，共同调节血管生成的进行，大体上可将这些调节因子分为正性调节因子和负性调节因子，正负调节的总体平衡决定着血管生成过程的启动与关闭。

Above all, his description of Divine wisdom bespeaks as to conception, style, and manner of presentation, a later stage of Alexandrian thought than that found in Wisdom.

最重要的是，他的描述神圣智慧bespeaks的概念，风格，以及以何种方式表述，后一阶段的亚历山大认为比中发现的智慧。

Metformin can inhibit the growth of lung adenocarcinoma cell lines significantly and potentiate cisplatin in a dose- and time-dependent manner in vitro. Cell-cycle progression is arrested in G1 stage and apoptosis is indued after metformin treatment.

二甲双胍可以使细胞周期循环阻滞于G1期，并诱导细胞的早期凋亡增加，从而有效抑制肺腺癌细胞株的体外增殖，其作用呈浓度及时间依赖性增强；二甲双胍与顺铂共同作用可以增强对肺腺癌细胞生长的抑制作用。

At this stage, the human body is hardly any symptoms and feel, this is the real cancer early; by the size of a grain of rice to grow into almond-shaped only a year or so, the mid-known cancer, symptoms must be the human body and Reaction; if not discovered and treated in a timely manner, to the development of advanced cancer only a short period of a few months.

在这个阶段，人体几乎没有任何症状和感觉，这才是真正的早期癌症；由米粒大小长成到杏仁状只需要一年左右的时间，称为中期癌症，人体会有一定的症状与反应；如果不能及时发现与治疗，发展到晚期癌症只需要短短的几个月的时间。

Multiplication rate achieved with solid/liquid alternate manner. 7. The late stage proembryos were induced in EC of multiplication medium with 9,000 mg·L-1myo–inositol and 0.1 mg·L-1 IBA.

含有50 g·L~（-1）蔗糖和0.1 mg·L~（-1）IBA的DCR1培养基最适合白皮松体细胞胚的成熟诱导，该组合明显提高了白皮松体细胞胚的成熟频率。

A rattling tempest was churned up as the percussionists drummed three huge scrolls of paper which cascaded from the ceiling to the stage floor, while intriguing sounds were created with all manner of paper products, from cardboard tubes and boxes to pompoms, paper bags and a paper umbrella...

演出现场，三个从天花板垂落至舞台的巨大纸轴俨然成了打击乐器，在打击乐手的敲击下，翻滚、跃动的纸轴营造出一场惊天动地的暴风雨。

Simultaneously, the children's content of teaching in school and the manner of education at home will also be projected on stage.

相同的，导师教导的内容、父母教育的模式，都将透过儿童的表演，进行一场残酷的挑战。

In recent years, the Educational department has released a series of documents, heavy before highly school inspector, bilingual education development, before large quantities of financial funds starts to study, the bilingual education incline, more and more national minority guardians request the child before study the stage to accept the bilingual education.in view of this, the author had near 20 year bilingual education experience's country take one to manage the bilingual kindergarten to conduct the research as the object of study, this article mainly introduced before the domestic and foreign study, bilingual education's research results, from the baby (study interest, in garden use Chinese's frequency), the guardian (effective way which to child bilingual support dynamics, adopted, understood child's way, bilingual manner), the teacher (disposition, use Chinese's condition, Chinese proficiency, bilingual teaching form and ability, basic quality enhanced demand and way) and the kindergarten (the teaching hardware facility provided, the bilingual teachers' raise and training, the bilingual environment, the bilingual teaching material) four aspects elaborate its Red Flag kindergarten bilingual education present situation, with bilingual education mutual influence.

近年来，教育部门出台了一系列文件，高度重视学前双语教育的开展，大批财政资金开始向学前双语教育倾斜，越来越多的少数民族家长要求孩子从学前阶段接受双语教育。鉴于此，笔者以一所拥有近二十年双语教育经验的国办双语幼儿园为研究对象进行研究，本文主要介绍了国内外学前双语教育的研究成果，从幼儿(学习兴趣、在园使用汉语的频率)、家长(对儿童双语的支持力度、采取的有效方式、了解孩子的途径、双语态度)、教师(配置、使用汉语的状况、汉语水平、双语授课形式和能力、基本素质提高的需求和途径)和幼儿园(教学硬件设施配备、双语师资的培养和培训、双语环境、双语教材)四个方面阐述其红旗幼儿园的双语教育现状，与双语教学的相互影响。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。