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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Novel and lively study form makes learning process happy and humor, the domestic education atmosphere of relaxed harmony is in close child form stage by stage in interactive study.

新奇活泼的学习形式则使学习过程愉快而风趣，轻松和谐的家庭教育氛围在亲子互动的学习中逐步形成。

Chromatin hetropycnote partly in spermatogonium, then become highly coacervate to form chromosome at the stage of spermatid and at last decondense at the stage of spermatozoon.

染色质在精原细胞中为部分异固缩，在精母细胞中高度凝聚为染色体，在精细胞及精子中为均匀非致密态。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

The developing stage of American government administration is coincident with the developing stage of its natural resource management.

美国政府管制的发展阶段与国土资源管理的发展阶段吻合，管制理论影响了美国国土资源管理的历史演进过程。

The first part mainly deals with the first building stage of the British Empire in the 17th century, that is, the initial stage of the British Empire which is characterized by non-governmental colonial expansion.

本论文主体内容共分两个部分，第一部分着重论述了17世纪英帝国形成的第一阶段，即以民间拓殖为主要特色的英帝国初创阶段。

The passive continental marginal basins of South America formed in Late Jurassic-Early Cretaceous as Gondwana land splitting, underwent pre-rift stage, synrift stage, transitional period and drift episode, formed sets of generating rock and reservoir-cover rock compages.

南美洲被动大陆边缘盆地形成于晚侏罗世-早白垩世冈瓦纳大陆的裂解，经历了前裂谷期、同裂谷期、过渡期、后漂移期4个阶段，发育了多套生储盖组合。

Psychoanalysis a stage in psychosexual development when the child's interest is concentrated on the anal region; fixation at this stage is said to result in orderliness, meanness, stubbornness, compulsiveness, etc..

一个性心理的发展阶段，此时儿童的兴趣集中在肛门区域；固结在这一阶段被认为会导致秩序、吝啬、固执、强迫症等等。

The competition comprises of two stages namely conceptive plan at stage one and detail plan at stage two.

征集分两个阶段，第一阶段为概念性方案征集，第二阶段为方案深化设计。

Therefore, the moist potential vorticity analysis associated with the mesoscale convective system was researched in the paper, so as to reveal the moist potential characteristics of mesoscale convective system in typhoon circulation. The results showed that, during the generation stage, convective instability characterized by MPV1 was a favorable condition for forming MCS, while the vorticity which was caused by the slantwise isentropic surface and the vertical shear of the horizontal wind characterized by MPV2 was an inspiring mechanism. The specific process was that, the atmosphere exhibited the feature of strong convective instability at the low level of convection areas and the southeast areas, which included plenty of erratic energy. The slantwise ascended the stream transfer the erratic energy to northwest, where the stratification stability was small. As decreased, the cyclonic vorticity increased. On the other hand, the vorticity caused by the slantwise isentropic surface and the vertical shear of the horizontal wind inspired the release of convective instability and as a result that the MCS formed. During the development stage, the cyclonic vorticity increased quickly with the persistent decreasing of . Meanwhile, the inclination of isentropic linewas bigger than the absolute momentum line at middle level, which exhibited the feature of conditional symmetric instability , then the conditional symmetric instability was forced to release by the vorticity resulted from the slantwise isentropic surface and the vertical shear of horizontal wind, and the MCS developed.

结果表明，在对流形成阶段，MPV1即对流不稳定为MCS的形成提供背景不稳定条件，由MPV2即湿等熵面的倾斜和水平风的垂直切变而引起的涡旋发展作为强迫机制：MCS形成的区域及东南区域中低层是强对流不稳定层，蕴含丰富的不稳定能量，倾斜上升运动把对流不稳定区具有强不稳定能量的暖湿空气向西北中层的中性层结区输送，由于的减小，气旋性涡度增强，有利于形成对流，另一方面，由于湿等熵面倾斜和低空急流加强而引起的涡旋发展作为一种强迫机制激发对流不稳定能量得到释放，从而形成对流；在对流系统地发展阶段，由于低层的对流不稳定性进一步减弱，进一步减小，气旋性涡度进一步增强，有利于MCS的增强，中层等θe 线的倾斜度比绝对动量M 等值线的倾斜度大，对应有条件对称不稳定区域，满足条件对称不稳定条件，在湿等熵面倾斜和台风低空急流作用下引起的涡旋发展强迫对称不稳定能量释放，从而使得对流得以维持和加强。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。