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To study the regulation mechanism of SOCS-3 (Suppressor of cytokines signals-3) for erythropoietic development, small interference RNA expression vectors of SOCS-3 were constructed and transferred in to the K562 cell lines stably by lentiviral system.

为了研究细胞因子信号转导分子3(suppressor of cytokine signals-3，SOCS-3)对造血发育的影响，构建了SOCS-3慢病毒siRNA干涉载体，并转染人红白血病细胞株K562。

But when they expressed alone, cry26 gene leaded inclusion body formation within or outside of the exosporium, whereas cry28 gene only induced crystal formation outside of exosporium. All of the proteins could not stably exist.

而当两个cry基因单独存在时，cry26基因表达合成的内含体位置不确定，大部分在芽胞外壁外侧形成，少数在芽胞外壁内侧形成；而cry28基因则只在芽胞外壁外侧表达合成内含体。

In a word, this system can operate expediently, run stably, and achieve the intending purpose.

总体来看，此系统操作方便，运行稳定，达到预计的效果。

After our study, we got four new results. Firstly, it is feasible to use the wavelength stabilization feedback system to these high average power FELs. We can get stabilized wavelength lasers and the facilities can work stably.

我们得到了四点结论：首先，在这样高平均功率的FEL上加载稳定波长的反馈是可行的，能够很好的稳定住波长，并且系统能够稳定的工作。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

Decreased expression of MMPs by blocking the expression of Snail in ovarian carcinoma cells Objective: To investigate the influence of antiadhesive antibody (SHE78-7), targeted at the potent hemophilic cell adhesion molecule E-cadherin, on the motility and invasive ability of ovarian carcinoma cells that have stably tansfectant with anti-Snail plasmid.

第二部分反义Snail对裸鼠卵巢癌原位移植瘤转移的影响目的：观察转染反义Snail质粒的卵巢癌细胞在裸鼠原位移植瘤模型中生长及转移的情况，检测转移组织中Snail、E-钙粘蛋白、及基质金属蛋白酶的表达和分布。

Results of the histochemical and SDS-PAGE analysis showed that the target genes were stably inherited.

组织化学和 SDS-PAGE 检测结果表明，外源基因能够稳定遗传，功能得到正确表达。

However,content of stably combined humic substances was reduced,and five-C sugar was apt to ...

玉米秸秆和根茬对土壤的培肥作用存在差异。

This paper reviewed the research on dynamic and balance of soil nutrient pool under long-term fertilization conditions,mainly about some active aspects which included dynamic variations of total nitrogen,available N,total phosphorus,available P,total potassium,available K,and organic carbon in soil; dynamic variations of ammonium N,amino acid N,amino sugar N,unknown N,non hydrolyzable N and microbiomass N in soil nutrient pool; changes of Ca2-P,Ca8-P,Al-P,Fe-P,O-P,labile organic P,moderately labile organic P,moderately stable P,highly stable P and microbiomass P in soil; changes of loosely,stably and tightly combined humus,humic and fulvic acids in soil carbon pool; effects of long-term fertilization on organic-mineral complex status in soil; nutrients cycling and balance of agricultural soil nutrients pool.

扼要地概述了国内外近年来关于长期施肥条件下土壤养分库的动态与平衡方面的研究成果。主要介绍并讨论了土壤氮、磷、钾养分全量及有效量的动态变化，土壤有机碳含量的动态变化；铵态氮、氨基酸态氮、氨基糖态氮、酸解未知态氮、非水解残渣氮及微生物体氮在土壤氮库中的动态变化；Ca2 P、Ca8 P、Al P、Fe P、闭蓄态磷，活性有机磷、中度活性有机磷、中稳性有机磷、高稳性有机磷及微生物体磷在土壤磷库中的动态变化；土壤碳库中松结态腐殖质、稳结态腐殖质、紧结态腐殖质以及富里酸和胡敏酸的动态变化；长期施肥对土壤有机无机复合状况的影响；农田土壤养分库的养分循环与养分平衡。

We successfully got transgeneic HFCL-CEA cell strain, which can express immunogenic CEA effectively and stably.

成功地获得了高效稳定表达具有免疫原性的CEA蛋白的转基因细胞株HFCL-CEA。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。