查询词典 stably

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

RESULTS: In the SH-rhBMP-2 group, sparse chondroid tissue could be found at the 2 weeks of operation, which arranged orderly along the tibial tunnel from 4 to 8 weeks, tendon adhered to adjacent tissue stably, and bony tissue was appeared at the 12 weeks.

结果：透明质酸钠-重组人骨形态发生蛋白2亚组在术后2周隧道内见稀疏软骨样组织，4~8周时隧道内见沿骨隧道排列的软骨样组织，双股肌腱之间形成稳定组织连接。l2周时出现部分骨样组织包裹肌腱。

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后，将其亚克隆到质粒表达载体pIRES中，构建以内部核糖体进入位点相连的CD基因的质粒表达载体pIRES-CD，采用电穿孔法，以质粒表达载体转染ACC-2细胞，用400μg/mL的G418筛选10d，获得稳定表达CD基因的ACC-2细胞系，提取该细胞的总RNA，用RT-PCR检测CD基因的表达。

Results The practice has proved that CPR system runs normally, safely, stably and compatibly, Moreover, it is maintained easily.

结果通过上述技术建立起来的CPR系统已在我院34个临床科室使用。

We simulate this system in laboratory successfully. The system work stably and operate easily. It can obtain the satisfied performance and high stability, follows the source well. The time of control improve obviously. It improve the system antijamming ability and comprehensibly improve the dynamic performance of system.

该系统在实验室仿真成功，系统工作稳定，操作方便，能够获得满意的性能指标，具有较好的快速跟随性，且稳态精度高，调节时间有了明显改善，提高了系统的抗干扰能力，全面的改善了系统的动态性能。

The present invention can make the iron loss of oriented steel sheet stably reduce by 5%, and can prevent its surface coating from being damaged and curvedly deformed by means of heat-transfer and mechanical analysis and regulation of the parameters, including laser energy, power, pulse-width and scanning speed.

经传热和力学分析，调整激光能量，功率，脉宽，扫描速度等参数，实现对取向度硅钢片既稳定降低铁损5％，而又不发生表面涂层破坏和弯曲变形。

By the cooperation of 8-MOP/UVA, the photoaging-characteristic biological markers of the dermic fibroblasts in vivo and in intro were changed as follows:① with a permanent switch of mitotic to stably postmitotic phentypes, fibroblasts displayed growth suppression and morphological changes of cell senescence;② increasing expression of SA-β-galactosidase;③ increasing expression of p16 protein;④ continuous up-regulation of mRNA of MMP-1 and MMP-3, while the protein of TIMP-1 was only slightly induced;⑤ high levels of the 4977bp deleted mtDNA accumulated in dermis and cultured fibroblasts, and large accumulation of a A→C base transversion of 414 position of 〓 of human mtDNA control region for replication of cultured fibroblasts also.

2.8-MOP/UVA作用下，体内外真皮成纤维细胞生物学特性出现具有光老化特征性的改变：①细胞由具有分裂活性的分裂表型转化为不具有分裂能力的分裂后表型，形态学出现细胞衰老的相应改变；②SA-β-Gal表达增加；③p16蛋白表达增加；④MMP-1、MMP-3 mRNA持续表达而TIMP蛋白表达仅被轻微诱导；⑤mtDNA 4977bp缺失大量累积，培养成纤维细胞mtDNA复制控制区〓片段414碱基迅速出现大量A→C的点突变。

The echometer was used to monitor the change of the annular water level in the coal- bed methanewells production,which could decrease pressure stably and continuously.

在煤层气井排采过程中，通常用回声仪来监测环空液面变化，通过控制液面下降速度来实现稳定、连续降压。

Ltd is an professional IT service company with IT service code, and chain management mode And we commit ourselves to offer special, professional and high effective IT items epibolic service for family, company and public institution in order to ensure clients' IT products equipment and information system to operate effectively and stably.

公司前身为楚天IT 服务连锁，经技术力量全面提升和服务质量的加深优化、管理模式重新改革后重组形成更加强大科技公司。泽宇公司致力于为家庭、企业、厂商提供个性化、专业化、快速高效的第三方 IT 项目外包服务，以保障客户的 IT 产品设备、信息系统能高效、稳定的运行。

In S. erythraea, pZW could express reporter genes thiostrepton resistance gene and enhanced green fluorescin gene. And it is more suitable to express exogenous genes in S. erythraea stably.

在糖多孢红霉菌中，pZW可以表达硫链丝菌肽抗性基因和绿色荧光蛋白基因，是更适合在糖多孢红霉菌中稳定表达外源基因的整合型表达载体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。