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To elucidate the mechanisms regulating TSHb expression, we isolated a 5.1-kb fragment of the red-spotted grouper 5'-flanking region by Genome walking .1.8kb of this upstream region was used to produce GFP reporter construct (pBK-TOL2) for analysis of tissue-specific expression in zebrafish embryos.

将起始密码子上游1864bp的启动子片段顺向克隆到pBK-TOL2载体中， pTSHb-TOL2重组质粒与转座酶mRNA共注射斑马鱼一细胞期受精卵，荧光显微镜观察发现，TSHb启动子驱动GFP在原始生殖细胞和垂体中表达。

The MCP gene was amplified from Red-spotted Grouper Nervous Necrosis viral genome by RT-PCR. The recombinant expression vector pRSET A-MCP was constructed and transformed into BL21 (DE3) plysS to express proteins with induction in different media, at different pH, or at different temperatures.

方法］采用RT-PCR技术扩增出赤点石斑鱼神经坏死病毒MCP基因，构建重组表达载体pRSETA-MCP，以其转化大肠杆菌BL21(DE3)plysS，在不同培养基、温度、pH值条件下进行诱导表达。

The instant he spotted a troop of red-eared monkeys, they began to give hack calls and chirps, eyes wide with terror.

在Butynski发现一群红耳猕猴之后，它们立刻朝着他叫个不停，圆睁的眼睛里流露出恐惧的神情。

In the 1970s, while touring the California countryside, Lee spotted a crop duster sitting in a farmer's field and asked permission to take it aloft.

这位绝美婉约又有无比勇气的女子，有几张照片在中文网站上不曾见过，却在英文法文的传媒上寻到。

However, if the patrol police car has spotted the rabbit car, they may have started chasing the car.

但是，如果有巡逻警车发现兔子车，他们可能已经开始追车。

However, if the patrol police car has spotted the rabbit car, they may have started chasing the car.

但是，如果巡逻警车已发现兔子车，他们可能已经开始追车。

Hunkered down in the bow, White spotted two crew members approaching in an inflatable raft. The pair tossed over a rope so they could tow the disabled lifeboat behind them.

贴近收头桨，怀特两个船员在充气的救生艇上，他们两个投一条绳子所以能让不能用的救生艇在他们后面。

This time it was less blatant and was not spotted - if it had been, Portsmouth would have been down to 10 men - and Albion compounded their bad luck when Gera hacked the ball out of Kiely's clutches to present Kanu with a tap-in.

这个时候，它是那么明显，并没有发现-如果它一直朴次茅斯，将已回落至1 0男人-和姆维奇复杂自认倒霉时，格拉砍死球出k i ely的魔掌中，以目前的卡努与自来水中。

Since late last year, online tempests have blown up over a video of an official in Guangdong Province who assaulted a young girl and bragged that he was above punishment, and a Nanjing city official who was spotted wearing a $14,500 Vacheron Constantin watch and smoking $22-a-pack cigarettes, evidence of a lifestyle well beyond his means.

自去年晚些时候，网络上的一段录像显示一位广东省官员调戏一个小女孩并且吹牛不会受到惩罚而激起民愤，一位南京的官员戴天价表（价值14500美元的 Vacheron Constantin 手表）、抽22美元一包的天价烟，其行为明显超出了其收入水平。

As Comet Lulin moves into the northern sky in mid February to rise around midnight, it should at least be spotted by comet watchers with binoculars and a good sky chart.

彗星卢琳在2月中旬将移动到北变的天空，午夜中升起，彗星了望者用双目望眼镜和天体图应该就能将它定位。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？