查询词典 sporadic cholera

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Methods Serotyping and phage-biotyping systems were used to examine 130 V. cholerae non-O1 strains collected from some areas in Guangxi, and cholera toxin was detected by PCR.

方法收集广西部分地区130株非O1群霍乱弧菌进行血清学分型和噬菌体—生物分型，并用多聚酶链反应进行霍乱肠毒素检测。

The mid-19th century the European continent, the advent of the Great Revolution turmoil on the eve of Italy in the rule of the Austro-Hungarian Empire, the young Anzheluo plotting riots because the plan was brought to light and went into exile in France Aicheng, as childhood friend the betrayal, still can not escape were chased and killed by the Government of Austria sent destiny, in order to avoid killing, he inadvertently trapped in a plague-infested areas, the ravages of cholera that people fall into frenzy, Anzheluo been confusion is the misconception that the masses poisoning, has been the object of the chase strangling.

19世纪中叶的欧洲大陆，大革命风暴来临的前夕，意大利在奥匈帝国的统治下，年轻的安哲罗因为策谋暴动的计划败露而流亡到法国艾城，由于儿时朋友的出卖，依旧脱逃不了被奥地利政府派人追杀的命运，为了躲避追杀，他无意中陷入了一个瘟疫横行的地区，霍乱的肆虐使人们陷入狂乱，安哲罗被混乱的群众误认为是投毒者，成了被追捕绞杀的对象。

The researchers caution that they haven't shown conclusively that flies are in fact spreading the diseases. But more than 30,000 flies might enter a poultry house over six weeks. And flies are known to be vectors of viral and bacterial diseases such as cholera. Another study to add to the growing pile of research suggesting our cheap meat is not as cheap as it seems.

科研人员提醒：尽管这些样本还没最终认定此类疾病就是通过苍蝇传播的，但是一个养殖场在六个星期内就会有3万只苍蝇光顾，而众所周知，一些诸如霍乱的病毒和细菌类疾病就是靠苍蝇传播的。

Next,john snow look into the source of the water for these two streets.he found that it came from the river polluted by the dirty water from london.he immediately told the astonished people in broad street to remove the handle from the pump so that it could not be used.soon afterwords the disease slowed down.he had shown that cholera was spread by germs and not in a cloud of gas.

接下来，约翰雪调查这两条街的水的起源，他发现这水来自被伦敦来的污水污染过的河流，他立刻告诉宽街上惊恐的人们移开水泵的把手从而使他不能被使用，在这之后，很快这疾病就减缓了，他展示了霍乱是通过细菌来传播而不是一团气体。

Northern cold regions such as hair many arthritis; measles, meningitis, scarlet fever prevalent in winter and spring, cholera, dysentery and other gastrointestinal diseases many hair in the summer.

中国气象局国家气象中心应用气象室主任赵琳娜和中国疾病预防控制中心环境与健康相关产品安全所所长金银龙对此作出解读。

Article twenty-eighth suffering from plague, cholera and anthrax death, the body must be immediately disinfected the nearest cremation.

第二十八条患鼠疫、霍乱和炭疽死亡的，必须将尸体立即消毒，就近火化。

Mice then ate the transgenic rice in the form of powder, absorbing the cholera toxin B antigen.

然后，老鼠吃了这种转基因水稻磨成的粉，吸收了霍乱毒素B抗原。

GM1 gangliosidoses, the specific component in lipid raft, was used as the hallmark of lipid raft. The distribution and expression of GM1 were probed by horse radish peroxidase coupled to cholera β-subunit, and revealed by chemiluminiscent HRP substrates.

再以脂筏的特有化学成分神经节苷脂GM1作为脂筏的标志分子，利用霍乱毒素β亚基能与GM1特异性结合的特性，以辣根过氧化物酶标记的霍乱毒素β亚基用点印迹法化学发光检测神经节背脂的分布，从而对脂能进行定性鉴定。

Other major figures: Robert Koch (first person to isolate bacteria in pure culture; discovered the agents of cholera and the cause of tuberculosis, and used his own criteria to distinguish a bacterial culprit causing a disease from an innocent microbe); and Joseph Lister (who used carbolic acid spray to kill bacteria, insisted that antiseptics be used on hands, instruments, and dressings and made it safe to do major surgery).

其他的重要人物：Robert Koch（纯培养分离出细菌的第一人，发现霍乱试剂和结核病的原因，使用他自己的标准来区别致病菌的非致病{良性}菌）；还有Joseph Lister（使用石炭酸喷雾剂杀菌，坚持用消毒剂消毒手，器械和敷料，使其在大外科手术中更为安全）。

For you say, My doctrine is pure, And I am clean in Your eyes.

11：4 你说，我的道理纯净，我在你眼前清洁。

The method takes make water sedimentary in patient of be in hospital of leucocyte grow in quantity 30, according to clean middle make water bacterioscopy result, distinguish the patient to affect group and blame infection group, pathology of puncture of farther travel kidney analyses blame infection form.

方法取尿沉渣中白细胞增多住院患者30例，根据清洁中段尿细菌检查结果，将患者区分为感染组和非感染组，非感染组进一步行肾穿刺病理分析。