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In this paper, we present an access cell selection approach, which is based on cell sojourn time estimation. The approach selects access cell according to the cell sojourn time estimated. Compared with residual dwell time approach and power level offset approach, our access cell selection approach has a much smaller probabfiity of erroneous access cell selection, thus a better performance.

在本文中，我们提出了一种基于移动台小区驻留时间估计的接入层次选择方法，这种方法依据移动台在小区中的驻留时间估计值来进行接入层次选择，与剩余驻留时间方法与功率小区偏移方法相比，其小区选择错误概率明显要低，具有较好的性能。

Because of this situation, we suggested that the construction of human embryonic stem cell research regulations in Taiwan should be of first priority and illegal use of human embryo or unpatentable types of human embryonic stem cell inventions should be regulated by law. Moreover, following the trend of stem cell technology, the regulation of cell therapy, gene therapy and analysis or diagnostic technologies related to stem cells should be amended to support stem cell research and research foundation, research awards or preference of business investment should be established to improve development of stem cell industry in Taiwan.

据此，本研究建议我国应优先建制人类胚胎干细胞研究管制规范，并以法律明订违法使用人类胚胎之情况或不予专利之人类胚胎干细胞发明态样，另一方面配合干细胞技术的应用趋势，对於干细胞相关的细胞疗法、基因疗法以及干细胞特性检测等潜在发展领域进行相关规范之增修，并且针对干细胞产业设立研究基金、拟定奖励研究或优惠投资方案，以达到促进干细胞产业发展之政策目的。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Pathology findings:there were 294 cases of clear renal cell carcinoma (83.76%),including 2 cases of cystis renal cell carcinoma ,27 cases of Grannular renal cell carcinoma ( 7.69 %), 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma,10 cases of other cell types .

病理结果：透明细胞癌294 例(83.76%，其中囊性肾细胞癌2例)，颗粒细胞癌27 例(7.69%)，乳头状肾细胞癌14例(3.98%)，肾混合细胞癌6例(1.7%)，其它癌10例。

Pathology findings:there were 294 cases of clear renal cell carcinoma (83.76%),including 2 cases of cystis renal cell carcinoma ,27 cases of Grannular renal cell carcinoma ( 7.69 %), 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma,10 cases of other cell types . Followup was achieved in 263 cases (74.93％),with tie 3、5year and 10 year survival rates being 70.45 %(124/ 176),53.3%(40/75) and 23.8%(5/21) respectively.

病理结果：透明细胞癌294 例(83.76%，其中囊性肾细胞癌2例)，颗粒细胞癌27 例(7.69%)，乳头状肾细胞癌14例(3.98%)，肾混合细胞癌6例(1.7%)，其它癌10例。263 例(74.93 %)获得随访，随访时间6个月～12 年，3 年、5 年和10年生存率分别为70。

Pathology findings:there were, 294 cases of clear renal cell carcinoma （83.76 %）, including 2 cases of cystis renal cell carcinoma , 27 cases of Granntdar renal cell carcinoma （ 7.69 %）, 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma, 10 cases of other cell types .

病理结果：透明细胞癌294例（83.76%，其中囊性肾细胞癌2例），颗粒细胞癌27例（7.69%），乳头状肾细胞癌14例（3.98%），肾混合细胞癌6例（1.7%），其它癌10例。263例（74.93%）获得随访，随访时间6个月~12年，3年、5年和10年生存率分别为70.45%（124/176）、53.3%（40/75）和23.8%（5/21）。

Monocellular Raman spectrums of normal liver cell line (LO2) and carcinoma liver cell line (SMMC-7721) were studied with laser tweezers Raman spectrums system and each cell was investigated in three different points, the result of which showed; significant differences of average Raman spectrums were found between the normal cell and the carcinoma one; And, the spectra line of carcinoma cell became weak at the whole; Additionally, the spectral peak intensity ratio of normal cell at 1658cm^(-1) and 1450cm^(-1) was 0.63 while that of carcinoma was 0.99; Moreover, the structure and the amount of protein; nucleic acid, lipids and other such molecules had also changed.

本工作利用激光镊子拉曼光谱系统研究了人正常肝细胞株(LO2)和肝癌细胞株(SMMC-7721)的单细胞拉曼光谱，对于每个细胞在不同部位测三个点。实验结果显示：正常细胞和癌细胞的平均拉曼光谱存在显著差异；癌细胞谱线强度整体变弱；正常细胞的1658cm^(-1)处峰和1450cm^(-1)处峰的强度比值为0.63，癌细胞的为0.99；癌细胞的核酸、蛋白质、脂类等重要生物分子在结构或含量上都发生了不同的改变。

Furthermore,the changes of cell organelles realized different cell functions, which fiber for mechanical support,parenchyma cell for preservation and vessel for transportation.3.FE-SEM and DCR technique revealed the arrangements of CMfs on cell wall of fiber, parenchyma cell and vessel.Firstly,fiber cell formed multilamellate concentric structure with thin and thick lamellae alternate.

纤维、基本薄壁组织细胞、导管分子的终端分化就是PCD；并且PCD过程是一个具有较长周期的过程，周期长短比较为基本薄壁组织细胞＞纤维＞导管分子；三类细胞最后的凋亡决定于细胞内原生质体完全降解的时刻。

The results demonstrated that, by comparison with un-transgenic Roselle cell or callus, the critical plating cell density and critical initiating cell density of transgenic cell line were cut down 60% and 50%, respectively,the growth cycle of transgenic cell in suspension culture was shortened from 16 days to 12 days, and the specific growth rate of transgenic Roselle callus was raised 75%, the content of flavonoid compounds in transgenic Roselle cell line or callus was hardly altered.

研究结果表明，所获得的转基因玫瑰茄细胞系的临界植板细胞密度和临界起始细胞密度分别降低了60%和50%，悬浮细胞培养周期由16d缩短到12d，转化愈伤组织的比生长速率比对照提高了75%，转基因玫瑰茄细胞和愈伤组织中的花黄素含量与对照相比没有发生明显变化。

Objective To study the effect of chitosan in the prevention of fibrous scar formation in the epidural space after laminectomy.

目的 观察选择性脊神经后根切除术椎板切除后硬膜外几种防瘢痕粘连物质的作用并探讨其机制。

On his way to the lift, he looked back to us or was it me his eyes pointing?

当他要进电梯时，他回头看我们（或者说是我，他的眼光？