查询词典 spinal cord

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

The current study not only confirmed the up-expression of muscarinic acetylcholine receptor five subtypes in rat spinal cord during morphine dependence appears to be reg□lated by endogenous NGF and GDNF, but also demonstrated that 〓 and 〓 receptor in spinal cord and ml in supraspinal predominantly mediate the morphine withdrawal symptoms.

本研究首次证实吗啡依赖时脊髓M受体发生适应性表达增加，这种改变可能与GDNF和NGF表达有关。

Spinal cord injury is commonly encountered in clinical..Traumatic spinal cord injury can result in severe damage, leading to tetraplegia, or worse.

脊髓损伤(spinal cord injury，SCI)是临床常见创伤，常导致四肢截瘫等严重后果。

Chitosan films can be easily biodegraded and has good biocompatibility in the spinal cord, which can be used in repairing spinal cord injury.

壳聚糖膜易于降解并与脊髓组织有良好的生物相容性，是一种可应用于脊髓损伤修复的生物材料。

Objective The changes of some neuropeptides were observed in brain slices of rats with spinal cord damage. Methods The experimental rats were divided into five groups:spinal cord occupied,contused,transected,false-operation groups and normal control.24 hours after operation,all animals were sacrificed,and brain slices produced.

随着神经系统神经肽的发现，针对神经肽功能以及通过神经肽变化来明确神经系统病理生理机制的研究越来越多［1］；因而，在脊髓损伤领域，神经细胞继发性改变中神经肽的作用也有许多研究［2］，但脊髓损伤后大脑中枢神经肽的改变尚无文献报道。

To observe the survival and migration of Bone Marrow Mesenchymal Stem Cells derived from Green Fluoresent Protein Transgenic mice and the change on expression of Fas and Fasl apoptosis factors at different times after transplantation GFP-MSCs via caudal vien to treat contused spinal cord injuryed adult SD rats modal ,and to provide experimental evidence for the reparing function and mechanism which MSCs treat spinal cord injury.

通过静脉移植绿色荧光蛋白转基因小鼠骨髓间充质干细胞治疗大鼠脊髓挫伤模型，观察骨髓间充质干细胞存活迁移情况及凋亡因子Fas和Fas1在移植后不同时点的变化情况，为骨髓间充质干细胞治疗脊髓损伤及机理提供实验室依据。

30Th day after spinal cord hemisected, the spinal cord and brain were taken from all rats, were sectioned with cryostat and were stained by NADPH enzyme histochemistry and neutral red.

术后30d取出脊髓和大脑行冷冻切片，做NADPH酶组化染色和中性红染色。

Objective:To study the recovery of neural function of adult rats injured spinal cord by fetal spinal cord tissue with vascularized tissue transplantation.

目的：研究提供血运的胚胎脊髓移植对成鼠损伤脊髓功能恢复的作用。

Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2（Ata2）; Peroxiredoxins 5（prd 5）; cysteine proteinase inhibitor cystatin c

国家自然科学基金；广东省自然科学基金人发角蛋白；脊髓损伤；大鼠；组织工程学；组织工程化脊髓；桥接物；形态学；行为学；神经元；轴突；神经纤维；髓鞘；星形胶质细胞；少突胶质细胞；小胶质细胞；室管膜细胞；神经干细胞；巨噬细胞；多核巨细胞；溶酶体酶；泛素肽酶系；抑制消减杂交；基因表达；细胞色素氧化酶Ⅲ；氨基酸转运蛋白系统A2；抗过氧化物蛋白5；半胱氨酸蛋白酶抑制剂

Methods Forty rats with spinal cord injury were made with Allen weight drop and spinal cord tissues were removd from 1 h to 4w after injury.

采用原位末端标记法，对大鼠脊髓损伤不同时间脊髓组织中凋亡细胞的数目和部位进行观察和分析，并作正常对照。

The construction enterprises of all completed construction projects to be itemized and in the investigation of the existence of peasant workers wages owed projects to be closed immediately; At the same time we must establish internal labor workers in the enterprise dedicated reserve system as contingency funds, devoted to the settlement of arrears of wages and the workers have unexpected events.

各建筑业企业对所有已竣工和在建工程要进行逐项排查，对存在拖欠农民工工资的工程项目，要立即予以结清；同时要在企业内部建立劳务用工专用储备金制度，作为应急资金，专门用于解决因拖欠民工工资而产生的突发事件。

Happy byrthday to YUK!!!!

极度迟来的生日快乐！！！！