查询词典 spin-tickling

While the sheave on most pulleys will not "spin," it should still turn freely.

这是对滑轮的描述，不知spin和turn怎么区别好

Saudi Arabia's promise to open the oil spigot is nothing but spin.

"沙特阿拉伯说了不算，就是在SPIN"，呵呵，这表达很形象，也是与"忽悠"相当接近的意思。

Or have Democrats yet again been elessly or traitorously spin at very high speed!

是不是民主党人又在无耻地或是背叛国家利益起劲地spin！

Or have Democrats yet again been sham elessly or traitorously spin at very high speed!

是不是民主党人又在无耻地或是背叛国家利益起劲地spin！

Or have Democrats yet again been shamelessly or traitorously spin at very high speed!

是不是民主党人又在无耻地或是背叛国家利益起劲地spin！

"P" is aspirated in"pin" but not in"spin".

中的p要送气发音，但spin中的p不送气发音。

In Chapter 2,we give some preparations about the basic knowledge of 4-manifolds, spin 4-manifolds and group actions on manifolds....

主要介绍了4-流形的基本概念，4-流形上的Spin几何以及流形上的群作用等方面的基本知识。

SPIN is a famous tool for analyzing and retrying the logical consistency of concurrent sys- tem.

SPIN 是一种著名的分析验证并发系统逻辑一致性的工具。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

In the work, Spin-on-dopants, as dopants, thermally diffused in the rapid thermal processor with different dwell time, temperatures, and ambients.

实验中使用液态旋涂磷源(Spin-on-dopants，SOD)做为掺杂剂，利用RTP技术在不同时间、温度和气氛下进行了快速热扩散。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失