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This suggests that CaHIRA plays a role in the early embryo development and the late stages. The different expression characters of hira gene in these fish species may be related to the different reproduction models. The research on Drosophila has indicated hira gene related to sperm nucleus decondensation during fertilization. The high expression of hira gene before fertilization is prepared for the sperm nucleus decondensation and the formation of male prenucleus because colored crucian carp is bisexual reproduction. However, the sperm nucleus is not decondensated during the fertilization and is only to activate egg nucleus in gynogenetic gibel carp.

在彩鲫中,从未受精卵到胚胎发育的各个时期均可检测到表达,但表达量发生明显变化,受精后表达量迅速下降,直到原肠胚期表达量有所上升,并一直保持到出苗。hira基因在两亲缘种成熟卵子受精前后表达情况不同,可能与两种鱼不同的生殖方式有关。

Puberty when the hypothalamus pituitary development formed to stimulate the beginning of testicular gonadal hormone secretion, so that in the vas deferens spermatogonium split into spermatocytes, and then split into sperm cells, sperm cells eventually split into sperm.

青春期时,丘脑下部垂体的发育形成了,刺激睾丸性腺开始分泌性激素,使输精管里的精原细胞分裂成精母细胞,再分裂成精子细胞,最终精子细胞分裂成精子。

The spermatophorins are transferred into the female sperm receptive organs and take part in the forming process of the spermatophore. During mating, the functional proteins transferred with the sperm can decrease female receptivity, increase egg production, and promote sperm storage and competition.

精包结构蛋白参与精包的形成;功能蛋白在交配过程中随精子一起转移到雌虫体内,导致雌虫行为和生理的深刻变化,如降低雌虫再交配率、提高产卵量、促进精子转移、储存和竞争等。

The semen analysis prior to treatment reealed that none of the men had normally shaped sperm and 213 of the men had subnormal sperm counts or sperm motility.

术前的精液分析显示所有人都没有正常形态的精子,且有213人精子计数和精子活性低于正常。

In present study, we found that Cipangopaludina chinensis has two kinds of sperm, one is the viable typical sperm, and the other is the unviable atypical sperm.

结果表明:中国圆田螺的精子具有双态现象,即既有可育的典型精子,又有不育的异型精子。

PartⅡFeasibility of vitrifying normospermic samples using droplets without cryoprotectans Objective Discuss the feasibility of droplets to vitrify normospermic samples without cryoprotectants. Methods Normal prepared sperm were frozen by conventional freezing protocol and non-cryoprotectant freezing in droplets respectively. Freezing in droplets means freezing sperm in every 10ul drop. After thawing, compared the two groups'sperm mobilities.

第二部分微滴法无保护剂玻璃化冷冻正常精子的可行性探讨目的探讨在不添加冷冻保护剂的情况下微滴法玻璃化冷冻正常精子的可行性方法收集16份正常精液标本,上游处理后分别进行慢速冷冻和微滴法冷冻,微滴法即用拉细巴氏管将标本形成10ul的液滴,直接投入液氮内冻存,复苏后镜检其活动率。

The rate of Flow cytometry to analyse retinoic acid haploid is 19.3%.The sperm-like cells achieved could activate bovine oocytes by sperm injection, and the development rate of blastosphere was 20.36%. Conclusions SSCs of newly -born mice can be induced and differentiated into sperm-like cells and are also capable of activating oocytes.

本实验通过体外诱导新生小鼠SSCs分化为精子样细胞,探讨不同诱导方法的诱导效率;采用显微注射法将精子样细胞注射到卵母细胞和去核卵母细胞,探讨其体外重构胚的发育能力,为SSCs体外分化研究打下基础。

Correlation of seminal plasma that there is significiant between thetwo groups in sperm activity score, sperm viability, liquefy time, deformative rate and sperm morphogenesis.

按解脲支原体阳性和阴性进行分组对照分析,发现阳性组精子活力得分、存活率、液化时间、畸形率以及精子形态与阴性组有显著差异(P<0.01)。

Uses giemsa's dye and microscope observe bursa copulatrix sperm of silkworm directly, artificial insemination using the sperm, the studies shows: The seminal fluid is similar in the male moth seminal vesicle and in the female bursa copulatrix after mating 20 to 30 minute, and the seminal fluid is contains large number of eupyrene sperm bundles and not be activated.

采用姬姆萨染色及显微镜下直接观察家蚕交配囊中精液,并用交配囊精液进行人工授精,研究发现:交配20~30 min雌蛾交配囊中精液与雄蛾储精囊中精液一样,精液未被活化,含有大量有核精子束。

The microkemel and sperm cell abnomalities were analyzed. Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P<0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P<0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P<0.05),且各剂量组微核率随染毒浓度的增加而升高,呈现剂量-反应关系(r=0.937,P<0.01);中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P<0.05),且各剂量组精子畸形率随染毒浓度的增加而升高,呈剂量-反应关系(r=0.904,P<0.01),畸形精子中以无钩为最多,其次是香蕉形、尾折叠、双头,分别为4.5%、3.09%和1.32%,结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

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