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Supravital stains ; Sperm ; Eosin ; Trypan blue ; Nigrosin ; Mouse

小鼠精子;活体染色;伊红;锥兰;苯胺黑

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时,应用形态学观察法,研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化;应用免疫荧光细胞化学和蛋白质印迹分析的方法,研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达;应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化;应用交配试验,检测雄鼠受精能力及其致孕雌鼠的产仔数的变化,用以揭示热应激对雄鼠生殖机能的影响。

During the past 3 years, 2 independent experiments of sperm-mediated gene transfer in domestic silkworms were carried out respectively in 2000 and 2002 with the method direct injection of copulation thecae.

在同源重组绿色荧光蛋白表达载体pG350的基础上,以pMD18-T为质粒载体,用egfp基因替换gfp基因,构建了家蚕核型多角体病毒早期即刻蛋白基因启动子BmNPV-IE控制下的增强型绿色荧光蛋白表达载体pMD-Fib-IE-EGFP。

The research demonstrated that the sperm-mediated gene transfer method (direct injection of copulation thecae) can introduce foreign gene into silkworms, and that the foreign gene may be integrated in the chromosomes of silkworms with many copies in series and can be transmitted to G1 offspring.

本研究的结果证明了精于介导基因转移方法能将外源基因有效地导入并整合于家蚕基因组,导入的外源基因可能是以串联多拷贝的形式整合于家蚕基因组中,整合的外源基囚叶@传午G;代。

These include a decrease in sperm production by almost 50 percent, a three- to four-fold increase in testicular cancer, an increase in the incidence of cryptorchidism (undescended testes, a condition where the testes are not in their normal location in the scrotum) and hypospadias.

这些还包括:精子产生量下降大约50%,三倍到四倍的睾丸癌发病率,隐睾症发病率增高(没有下降的睾丸,睾丸没有在阴囊中的情况)、尿道下裂。

Besides, we found that the 5′ regions of Dnmt1 and Dnmt2 were nearly completely unmethylated in all normal adults, IVF fetuses, sperm and aborted clones.

此外,我们还发现在正常成体牛、体外受精胎儿、精子和克隆流产牛胎儿中, Dnmt1和 Dnmt2基因的5′端几乎均没有发生甲基化。

Larger male coconut crabs have a higher number of sperm in both the vasa deferentia and testis.

大的椰子蟹输精管和精巢内精子数目较高。

The programme, a dramatisation of the epic journey sperm undertake in their quest to fertilise the egg, reveals other fertility secrets.

这部纪录片戏剧化地讲述了精子使卵子受孕的伟大征程,同时还揭示了其他生殖秘密。

Chinook salmon ; sperm vitality ; reactivator ; artificial seminal plasma; pH value ; temperature

大鳞大马哈鱼;精子活力;激活剂;人工精浆; pH值;温度

In the field of general medicine men, as the viscosity of semen and the impact of increased rate of sperm activity and activities, and is an important cause of male infertility.

在男科医学领域中,由于精液粘稠度增高而影响精子的活动率和活动力,是导致男性不育的一个重要原因。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。

The shamrock is the symbol of Ireland and of St.

三叶草是爱尔兰和圣特里克节的标志同时它的寓意是带来幸运。3片心形叶子围绕着一根断茎,深绿色。