查询词典 sperm-nucleus

By using two color FISH with one euchromosome probe and one sex chromosome probe, the sperm with same color of two hybridization signals in one sperm head could be identified as aneuploidy sperm or diploid sperm.

若用1条常染色体探针和1条性染色体探针进行FISH，可以区别头部有2个相同颜色荧光杂交信号的精子属非整倍体精子或二倍体精子。

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

To make the wapiti sperm capacitated in vitro, we selected and used the method of sperm-up. By making the rate of acrosome reaction as a main index, and the sperm energy, the survial time and the fertilizated rate of wapiti sperm and hamster mature oocytes as auxiliary indexes, it were carried out to compare the effects of wapiti sperm capacitation in vitro under different temperature, different density of pyruvate sodium, caffeine and heparin and different culture medium.

对马鹿精子，使用上浮法诱导其获能，通过对顶体染色测定精子的顶体反应率并作为主要指标，同时以精子活力、精子存活时间和异种受精率为辅助指标，对精子的获能效果进行评价，以比较不同培养温度、丙酮酸钠浓度、咖啡因浓度、肝素浓度以及培养基对马鹿精子体外获能效果的影响。

A eupyrene sperm and an apyrene sperm combined together forming a normal mature sperm, and the function of apyrene sperm was helping eupyrene sperm to swim.

一个有核精子和一个无核精子联合在一起组成一个正常的成熟精子，其中无核精子的功能是帮助有核精子游动。

Results In lead poisoning group, the pathological changes in genital glands of mice were obtained; sperm count, linear movement sperm and live sperm rate decreased; the elevated motionless sperm, cacospermia and primary spermatocyte chromosome aberration rate were found. In Haierfu group, the pathological changes in genital glands of mice ameliated to different degree, sperm count, linear movement sperm and live sperm rate elevated, and motionless sperm, cacospermia and primary spermatocyte chromosome aberration rate decreased.

结果 醋酸铅染毒组雄性小鼠的生殖腺有不同程度的病理学改变，精子计数、直线运动精子数、活精子率下降，而静止不动精子数、精子畸形率和初级精母细胞染色体畸变率升高；海尔福治疗组小鼠的生殖腺病理学改变有不同程度的改善，精子计数、直线运动精子数、活精子率有所升高，而静止不动精子数、精子畸形率和初级精母细胞染色体畸变率有所降低。

This experiment first studied in the mature nutrient medium to increase the hormone to the porine ovocyte in vitro mature process in the chromosome configuration and the mature rate influence, by explored the porine ovocyte in vitro mature rule, enhanced in vitro raise maturity, was the ROSI technology system preparation high quality ovocyte; Then has carried on the experiment in view of the porine round sperm cell in vitro separation, through to the ROSI operation and ICSI (Intracytoplasmic sperm injection, the comparison which ICSI) operated studies has affected the ROSI operation the factor.

本试验首先研究了成熟培养液中添加激素对猪卵母细胞体外成熟过程中染色体构型和成熟率的影响，以探索猪卵母细胞体外成熟规律，提高体外培养成熟度，为ROSI技术体系准备优质卵母细胞；然后针对猪圆形精细胞的体外分离进行了试验，进而通过对ROSI操作和ICSI(Intracytoplasmic sperm injection，ICSI)操作的比较研究了影响ROSI操作的因素。

Sperm density, sperm motility, sperm progressive motility, sperm fast progressive motility and sperm abnormality rate of epididymides were observed.

附睾精子观察密度、活动率、前向运动、快速前向运动精子百分比和畸形精子百分比。

Each male ratwas paired with 2 female rats in proestrus when administration terminated. Femalerats were examined the next morning for the presence of sperm in vaginal smears andunderwent a cesarean section on day 13 of gestation. Then the reproductive indiceswere calculated as follows: copulation index, pregnancy index, and fertility index,which can evaluate male fertility directly. The sperm motility and morphology wereevaluated by computer-assisted sperm analysis, moreover, the caudaepididymal sperm ultramicrostructure were analyzed by transmission electronmicroscope, which can evaluate the influence on sperm maturation bydutasteride ; sperm survival rate was assessed by SYBR-14 and propidium iodidefluorescent staining; serumal dihydrotestosterone and testosterone of ratswere detected by enzyme-labeled immunoassay, which can evaluate the effecton androgenic levels; the weights of testes and epididymides, were determined byprecise electronic balance; histological examination of above tissues were evaluatedby HE staining and TEM, which can evaluate treatment on histology of reproductiveorgans by dutasteride.

给药结束后雄鼠与处于动情前期的雌鼠按2：1合笼，计算雌鼠的交配指数、受孕指数和生育指数，直接评价雄鼠生育力；采用计算机辅助精子分析系统分析大鼠附睾精子活力和形态，在此基础上合并使用透射电镜技术对各组附睾尾部精子进行透射电镜分析，评价度他雄胺对大鼠精子成熟的影响；采用SYBR-14和propidium iodide双重荧光染色计算精子存活率；采用Elisa法测定大鼠睾酮(testosterone， T)和双氢睾酮(dihydrotestosterone， DHT)血清浓度，评价度他雄胺对雄鼠雄激素水平的影响；采用精密电子天平对各组睾丸、附睾、前列腺和精囊进行称重，评价度他雄胺对大鼠生殖器官重量的影响；采用HE染色法对各组睾丸和附睾进行组织学分析，同时采用透射电镜技术对附睾上皮细胞超微结构进行分析，评价度他雄胺对大鼠主要生殖器官组织学的影响。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

Pig oocytes cultured in vitro for some time were inseminated by frozen–thawed ejaculated sperm. At specified times after insemination, sperm penetration, cell cycle progression and mitogen-activated protein kinase phosphorylation were evaluated. It was shown that:(1) oocytes at various maturational stages could be penetrated by sperm;(2) sperm penetration did not affect meiotic cell cycle progression;(3) sperm penetration of germinal vesicle oocytes and maturing oocytes did not alter MAPK phosphorylation; and (4) when premetaphase I and metaphase I oocytes, in which MAPK was activated, were fertilised, no evident MAPK dephosphorylation was detected as in metaphase II oocytes.

不同成熟阶段的猪卵与精子融合后体外培养发现：(1)不同成熟阶段的猪卵母细胞都可被精子穿透；(2)精子穿透不影响减数分裂细胞周期进程；(3)精子穿透GV期卵母细胞和正在成熟的卵母细胞不改变MAPK磷酸化：(4)已受精的前中期I和中期I卵母细胞的MAPK有活性，MAPK不像MII卵母细胞那样受精后发生磷酸化。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。