查询词典 sperm whaling

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

After discovery of sperm capacitation,it has made some progress in the aspect of the mechanism of sperm capacitation,yet there are some problems that need future research.

自发现精子获能现象以来，对精子获能机制的研究已取得一些进展，但仍有不少问题需要进一步研究。

It can succeed in making wapiti sperm capacitation in vitro by use of the method sperm-up.

采用上浮法可使马鹿的精子体外获能。

Sperm capacitation is an essential mature process before mammalian fertilization. Making sperm capacitated in vitro is an important prerequisite to complete fertilization in vitro.

精子获能是哺乳动物受精前必须经历的一个成熟过程，所以精子的体外获能便是实现体外受精的一个重要前提。

Objective:To study the mechanism of uPA improving sperm capacitation by investigating the effect of uPA on the mitochondrial function of mouse capacitated-sperm in vitro.

目的：检测尿激酶型纤溶酶原激活因子体外对小鼠获能精子线粒体膜电位的影响。

Analysis of ovine sperm capacitation was assessed by different sperm staining types with CTC.The results showed that the percentage of capacitated sheep sperms in each treatment group ...

结果显示，在240 m in和360 m in时，各实验组中获能精子的百分数均明显高于对照组的(P.05)，说明一定浓度的孕酮有利于绵羊精子的体外获能。

In mammals, CRISP-1 inhibits sperm-egg fusion and can suppress sperm capacitation.

在哺乳动物中 CRISP-1抑制精子获能和精卵融合，但其分子机制还不清楚。

We detected the frozen-thawed sperm of 36 Simmental,23 Charolais and 12 Limousin,the results showed that the frozen-thawed sperm of these three breeds could penetrate the zona-free ova of hamster,and formed male pronucleuses.

对36头西门塔尔、23头夏洛来和12头利木赞牛冷冻精子的实验结果表明：三个品种的牛冻精均能穿入去透明带仓鼠卵，并形成发育良好的雄原核。

His work opened the way to intracytoplasmic sperm injection,in which a single sperm is injected directly into the egg cell.

他开创了细胞质内注射的先河，即把一个单精子直接植入一卵细胞内。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48