查询词典 sperm nucleus

When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后，同A组相比：E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高，在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著，在卵泡、黄体中阳性产物表达均较A组显著降低，在子宫中表达较A组变化不明显；同B组相比：E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高，在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低；同C组相比：E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高，在室周核、黄体中均显著降低；同D组相比：E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高，在视上核、弓状核显著降低，在其它部位表达变化差异不显著。

Neuronal cell bodies and fibers with moderate mGluR7-LI were seen in the olfactory bulb,anterior olfactory nucleus,piriform cortex,septofimbrial nucleus,bed nucleus of the strial terminalis,lateral hypothalamic area,paraventricular hypothalamic nucleus,supramammillary nucleus,medial and lateral mammillary nuclei,most part of the thalamus,medial and lateral geniculate bodies,nucleus of the optic tract,red nucleus,substantia nigra,interpeduncular nucleus,pontine nuclei,lateral parabrachial nucleus,superior olivary complex,nucleus of the trapezoid body,motor nucleus of the trigeminal nerve,facial nucleus,ambiguus nucleus,cochlear nucleus,vestibular nuclei,nucleus of the solitary tract,hypoglossal nucleus,prepositus hypoglossal nucleus,medullary reticular formation,Purkinje cells of the cerebellum,nucleus raphe obscurus,intermediolateral nucleus of the spinal cord,Onuf′s nucleus and lamina X of the spinal cord.

呈中等强度染色的神经元胞体和纤维见于嗅结节、前嗅核、梨状皮质、隔伞核、终纹床核、下丘脑外侧区、下丘脑室旁核、乳头体上核、乳头体内、外核、丘脑大部分核团、内外侧膝状体、视束核、红核、黑质、脚间核、桥核、臂旁外侧核、上橄榄复合体、斜方体核、三叉神经运动核、面神经核、疑核、耳蜗核、前庭核簇、楔束外核、孤束核、舌下神经核、舌下神经前置核、中缝隐核、延髓网状结构、小脑蒲肯野细胞层、脊髓中间带外侧核、Onuf核和中央管周围灰质。

When aborted rats were injected with 10 IU/g IFN-γin group D, compared with group A, expression of TNF-αinmmuopositive substance in the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami were highly increased, conversely, the ones in the nucleus pre-opticus suprachiasmaticus, neurohypophsis, adenohypophysis, ovary were obviously decreased, the change in the uterus was not remarkable; Compared with group B, expression of TNF-αin the arcuatus hypothalami was obviously enhanced, while in the nucleus pre-opticus suprachiasmaticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, neurohypophsis, pars intermedia, ovary, the results were on the contrary, the uterus had no significantly variation; Compared with group C, expression of TNF-αin the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and pars intermedia had an obviously risen trend, however, the variation in the nucleus pre-opticus suprachiasmaticus, nucleus periventricularis hypothalami, neurohypophsis, pars intermedia, follicle and corpus luteum was completely opposite, uterus had no obviously change.

外源腹腔注射10 IU/g IFN-γ后，同A组相比：D组TNF-α免疫阳性产物在下丘脑视前内侧核、视上核、视前大细胞核、室周核、室旁核、弓状核显著增高，在视交叉上核显著降低，在其它核团较A组无显著差异，在神经垂体、垂体前叶、卵巢中阳性产物表达均较A组显著降低，在子宫中的表达较A组变化不明显；同B组相比：D组TNF-α免疫阳性产物在弓状核显著增高，在视交叉上核、视前大细胞核、室周核、室旁核、腹内侧核、背内侧核、神经垂体、垂体中间部、卵巢各部阳性物质表达均显著降低，在子宫表达变化不显著；同C组相比：D组下丘脑视前内侧核、视上核、室旁核、弓状核、垂体前叶与中间部均显著增高，在视交叉上核、室周核、神经垂体、卵泡、黄体中均显著降低，子宫中表达变化不明显。

The result showed that IFN-γ was distributed over 20 nucleus of the hypothalamus, such as nucleus mamillaris medialis, nucleus pre-opticus medialis, area anterior hypothalami, nucleus ventromedialis, nucleus reunions, nucleus paraventricularis, nucleus supra optic, and etc. Many of IFN-γ positive nerve fibers were found in supra optic nucleus, paraventricular nucleus, nucleus mamillaris medialis and etc. Nerve fibers and astrocytes IFN-γ immunoreactive positive cells spreaded in paraventricular nucleus, nucleus pre-opticus lateralis, nucleus pre-opticus medialis and etc. At the same time, IFN-γ is also observed obviously in adenohypophysis and neurohypophysis. IFN-γ also expresses in follicular and corpus luteum.

结果显示，IFN-γ分布于乳头体内侧核、视前内侧核、下丘脑前区、腹内侧核、连接核、室旁核、视上核等20个核团中，各部分核团细胞着色程度深浅不一；在视上核、室旁核分泌部乳头体内侧核等有大量的阳性神经纤维；同时在室周核、视前外侧核、视前内侧核等13个核团中散布有一些阳性星形胶质细胞，与阳性神经细胞交错共存；腺垂体中腺细胞和神经垂体中神经纤维均有IFN-γ表达；卵泡和黄体中也有IFN-γ存在。

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A：正常精子(精子头部有1个红色荧光信号者为X精子，有1个绿色荧光信号者为Y精子)；B：染色体数目异常精子两个绿色荧光信号同在1个精子核内，可为Y双体精子或二倍体精子，若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交，可以区别该精子属Y双体精子或二倍体精子

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

In the brain of adult rat, the positive immunohistochemical product of lSL-l (ISL-l-positive) was mainly located in the neuronal nucleus and found in discrete regions except to brain cortex, such as the Purkinje cell layer and the granular cell layer of cerebellum, the granular cell layer and the pyramidal cell layer of hippocampus, the mitral cell layer, the internal and external plexiform layer, the granular cell layer and the granular cell layer of olfactory bulb and so on, and several nuclei of the hypothalamus, midbrain and pons, such as claustrum, anterior olfactory nucleus, accumbens nucleus, caudate-ptamen, pallidum, substantia nigra, striatum, islands of Callaje, mammillary nucleus, anterior pretactal nucleus, habenular nucleus, amygdaloid nucleus, cuneate nucleus, rubral nucleus, gigantocellular reticular nucleus and so on.

在正常成年大鼠脑中，同源框基因islet-1表达产物（ISL-1）免疫组织化学阳性物质广泛分布于除大脑皮层外的神经细胞的细胞核内，ISL-1阳性神经元密集分布于小脑Purkinje细胞层和颗粒细胞层、海马的颗粒细胞层和锥体细胞层、嗅球的内丛层、外丛层、颗粒细胞层及僧帽细胞层等，另外在丘脑、中脑和桥脑的一些重要神经元核团均有分布，如，屏状核、前嗅核、伏核、尾壳核、苍白球、黑质、纹状体、Calleja岛、乳头体核、前顶盖前核、缰核、杏仁核、楔束核、红核网状巨细胞核等。

When the anesthetic dose of propofol was 100 mg/kg, FOS-IR neurons appeared in the CNS, including piriform cortex, accumbent nucleus, lateral septal nucleus, paraventricular hypothalamic nucleus, ventral lateral geniculalaten nucleus, dorsal endapiriform nucleus, supraoptic nucleus, suprachiasmatic nucleus, anteroventral preoptic nucleus, nucleus of the solitary tract, and supramammillary nucleus, etc.

结果 C组可见部分散在FOS-IR阳性神经元分布，P组FOS-IR阳性神经元数比C组明显增多（P.01），并呈核特异性分布，S组在杏仁基底外侧核、丘脑室旁核、外侧缰核及海马回嗅觉小岛等核团发现FOS-IR阳性神经元较P组分布增多（P.01）。

ER, NGF and ChAT immunoreactive productions were widely presented in diencephalons, mainly in nucleus habenula, nucleus corpus geniculatum lateral, nucleus medialis thalami, nucleus lateral thalami, nucleus paraventricularis, nucleus paratenialis, nucleus rhomboidens, grisea periventricularis hypothalami, nucleus periventricularis hypothalami, nucleus paraventricularis hypoythalami, nucleus ventromedialis hypothalami, anterior region and posterior region hypothalami.

老年大鼠和去卵巢大鼠端脑大部分区域内ER、NGF和ChAT的表达活性及阳性细胞数目均显著下降，而补充17β－雌二醇后得到回升，并基本恢复到正常水平，表明雌激素通过ER对端脑内NGF和ChAT的表达有促进作用，这种作用可能部分地与雌激素对NGF的上调而作用于胆碱能神经元有关，也可能是雌激素通过ER直接调控了ChAT的表达，增强神经信息的传递。

Results The Fos reactive products were distributed in the piriform cortex,accumbens nucleus,bed nucleus of stria terminalis, cingulate gyrus,nucleus of diagonal band, hypothalamic paraventricular nucleus, supraoptic nucleus, suprachiasmatic nucleus, arcuate nucleus, central amygdaloid, subfornical organ, paraventricular thalamic nucleus, lateral habenular nucleus,ventrolateral part of the central gray,lateral parabrachial nucleus,locus ceruleus,medullary visceral zone.

结果 Fos阳性产物分布于梨状皮质、伏核、终纹床核、扣带回、斜角带核、下丘脑室旁核、视上核、视交叉上核、弓状核、中央杏仁核、穹窿下器、丘脑室旁核、外侧缰核、中脑中央灰质腹外侧区、脑桥臂旁外侧核、蓝斑、延髓内脏带等脑区，而在大脑白质及小脑中无明显的密集分布区。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48