查询词典 sperm center

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A：正常精子(精子头部有1个红色荧光信号者为X精子，有1个绿色荧光信号者为Y精子)；B：染色体数目异常精子两个绿色荧光信号同在1个精子核内，可为Y双体精子或二倍体精子，若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交，可以区别该精子属Y双体精子或二倍体精子

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

Results In lead poisoning group, the pathological changes in genital glands of mice were obtained; sperm count, linear movement sperm and live sperm rate decreased; the elevated motionless sperm, cacospermia and primary spermatocyte chromosome aberration rate were found. In Haierfu group, the pathological changes in genital glands of mice ameliated to different degree, sperm count, linear movement sperm and live sperm rate elevated, and motionless sperm, cacospermia and primary spermatocyte chromosome aberration rate decreased.

结果 醋酸铅染毒组雄性小鼠的生殖腺有不同程度的病理学改变，精子计数、直线运动精子数、活精子率下降，而静止不动精子数、精子畸形率和初级精母细胞染色体畸变率升高；海尔福治疗组小鼠的生殖腺病理学改变有不同程度的改善，精子计数、直线运动精子数、活精子率有所升高，而静止不动精子数、精子畸形率和初级精母细胞染色体畸变率有所降低。

Each male ratwas paired with 2 female rats in proestrus when administration terminated. Femalerats were examined the next morning for the presence of sperm in vaginal smears andunderwent a cesarean section on day 13 of gestation. Then the reproductive indiceswere calculated as follows: copulation index, pregnancy index, and fertility index,which can evaluate male fertility directly. The sperm motility and morphology wereevaluated by computer-assisted sperm analysis, moreover, the caudaepididymal sperm ultramicrostructure were analyzed by transmission electronmicroscope, which can evaluate the influence on sperm maturation bydutasteride ; sperm survival rate was assessed by SYBR-14 and propidium iodidefluorescent staining; serumal dihydrotestosterone and testosterone of ratswere detected by enzyme-labeled immunoassay, which can evaluate the effecton androgenic levels; the weights of testes and epididymides, were determined byprecise electronic balance; histological examination of above tissues were evaluatedby HE staining and TEM, which can evaluate treatment on histology of reproductiveorgans by dutasteride.

给药结束后雄鼠与处于动情前期的雌鼠按2：1合笼，计算雌鼠的交配指数、受孕指数和生育指数，直接评价雄鼠生育力；采用计算机辅助精子分析系统分析大鼠附睾精子活力和形态，在此基础上合并使用透射电镜技术对各组附睾尾部精子进行透射电镜分析，评价度他雄胺对大鼠精子成熟的影响；采用SYBR-14和propidium iodide双重荧光染色计算精子存活率；采用Elisa法测定大鼠睾酮(testosterone， T)和双氢睾酮(dihydrotestosterone， DHT)血清浓度，评价度他雄胺对雄鼠雄激素水平的影响；采用精密电子天平对各组睾丸、附睾、前列腺和精囊进行称重，评价度他雄胺对大鼠生殖器官重量的影响；采用HE染色法对各组睾丸和附睾进行组织学分析，同时采用透射电镜技术对附睾上皮细胞超微结构进行分析，评价度他雄胺对大鼠主要生殖器官组织学的影响。

City, the main stadium and some public enterprises and opening the stadium half-price: Workers Stadium, the stadium glory, Shichahai integrated Hall, Mu Xiyuan Tennis Center, Ditan Gymnasium, the Dongdan Sports Center, Sports Center on the altar, Guang'an Stadium, Chaoyang Sports Center, Haidian District Sports Center, Haidian District Stadium, Shijingshan Stadium, Shijingshan Stadium, Shougang Sports Center, Fengtai Sports Center Stadium in Tongzhou District, Daxing District Stadium, Changping District Sports Center, Shunyi District Sports Center, Huairou District Stadium , The Beijing Fengtai District Workers Club, Fangzhuang Sports Park, Old Bridge, Beijing property Xiaoyue Yuan's Sports Center, Beijing Yi Fang Sports Park City Health, Beijing-day gym brother Tony, Beijing Palace hot spring water in the world, Kang Hae-Beijing Aquatic Center Court, Beijing Kaiyuan city of entertainment and sports, fitness Sail park in Beijing, the Beijing-day River Hot Springs Sports Center, the Beijing Real Estate Development Co., Ltd.

市、区主要公共体育场馆及部分企业办体育场馆半价开放的有：工人体育馆、光彩体育馆、什刹海综合馆、木樨园网球中心、地坛体育馆、东单体育馆、月坛体育中心、广安体育馆、朝阳体育馆、海淀区体育馆、海淀区体育场、石景山体育场、石景山体育馆、首钢体育中心、丰台区体育中心、通州区体育场、大兴区体育场、昌平区体育中心、顺义区体育中心、怀柔区体育场、北京丰台区工人俱乐部、方庄体育公园、北京市古桥物业公司晓月苑康体中心、北京颐方园体育健康城、北京天贝兄健身房、北京南宫温泉水世界、北京康海苑游泳馆、北京开元康体娱乐城、北京市赛欧健身乐园、北京天水河温泉康体中心、北京怡海花园房地产开发有限公司健身俱乐部、北京浩沙健与美健身俱乐部第五分部、北京马莲道溜冰场，共33家。

To make the wapiti sperm capacitated in vitro, we selected and used the method of sperm-up. By making the rate of acrosome reaction as a main index, and the sperm energy, the survial time and the fertilizated rate of wapiti sperm and hamster mature oocytes as auxiliary indexes, it were carried out to compare the effects of wapiti sperm capacitation in vitro under different temperature, different density of pyruvate sodium, caffeine and heparin and different culture medium.

对马鹿精子，使用上浮法诱导其获能，通过对顶体染色测定精子的顶体反应率并作为主要指标，同时以精子活力、精子存活时间和异种受精率为辅助指标，对精子的获能效果进行评价，以比较不同培养温度、丙酮酸钠浓度、咖啡因浓度、肝素浓度以及培养基对马鹿精子体外获能效果的影响。

A eupyrene sperm and an apyrene sperm combined together forming a normal mature sperm, and the function of apyrene sperm was helping eupyrene sperm to swim.

一个有核精子和一个无核精子联合在一起组成一个正常的成熟精子，其中无核精子的功能是帮助有核精子游动。

Sperm density, sperm motility, sperm progressive motility, sperm fast progressive motility and sperm abnormality rate of epididymides were observed.

附睾精子观察密度、活动率、前向运动、快速前向运动精子百分比和畸形精子百分比。

Pig oocytes cultured in vitro for some time were inseminated by frozen–thawed ejaculated sperm. At specified times after insemination, sperm penetration, cell cycle progression and mitogen-activated protein kinase phosphorylation were evaluated. It was shown that:(1) oocytes at various maturational stages could be penetrated by sperm;(2) sperm penetration did not affect meiotic cell cycle progression;(3) sperm penetration of germinal vesicle oocytes and maturing oocytes did not alter MAPK phosphorylation; and (4) when premetaphase I and metaphase I oocytes, in which MAPK was activated, were fertilised, no evident MAPK dephosphorylation was detected as in metaphase II oocytes.

不同成熟阶段的猪卵与精子融合后体外培养发现：(1)不同成熟阶段的猪卵母细胞都可被精子穿透；(2)精子穿透不影响减数分裂细胞周期进程；(3)精子穿透GV期卵母细胞和正在成熟的卵母细胞不改变MAPK磷酸化：(4)已受精的前中期I和中期I卵母细胞的MAPK有活性，MAPK不像MII卵母细胞那样受精后发生磷酸化。

By using two color FISH with one euchromosome probe and one sex chromosome probe, the sperm with same color of two hybridization signals in one sperm head could be identified as aneuploidy sperm or diploid sperm.

若用1条常染色体探针和1条性染色体探针进行FISH，可以区别头部有2个相同颜色荧光杂交信号的精子属非整倍体精子或二倍体精子。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48