查询词典 sperm cell

According to the results of semen examination, can be classified as azoospermia and severe oligospermia, oligospermia, infertility and normal sperm count, more than sperm and sperm can not afford such disease.

根据精液检查的结果，可分类为无精子症、重度少精子症、少精子症、精子数正常性不育症、多精子症以及精子无力症等。

Objective: Retrospective study of the results of ICSI(intracytoplasmic sperm insemination)with frozen sperm obtained by PESA( percutaneous epididymal sperm aspiration ) was performed in 27 patients.

目的：回顾性分析27例无精子症患者经皮附睾穿刺取精术所获精子冷冻复苏后行卵细胞胞质内单精子注射治疗后的效果及妊娠结局。

Methods: The sperm morphology and sperm acrosin activity were analyzed by automated sperm morphology analyzer and spectrocolorimetry.

精子形态和顶体酶活性是决定精子能否受精的重要因素[1，2 ] 。

Spermatozoal SOD activity was positively correlated to sperm motility, normal morphology and sperm swelling by sperm hypo-osmotic...

提示精子SOD活性与精子结构、功能有关，可作为衡量精液质量的一个新指标。

To observe the spermic activity by using the microscope after mix ing fresh sperm with anti-sperm antibody,we daubed the hamster s vagina with anti-sperm antibody Gel once a day for 14 days,and then anatomised hamsters,obtain effective the vaginal tissue and observed the pathologic slices.

将抗精子抗体与新鲜精液直接混合后镜检；用导管将抗精子抗体凝胶涂布于地鼠阴道内，每天一次，两周后解剖做病理切片观察并评分。

A unique example of sperm cooperation in monotremes is used as evidence that the epididymis produces sperm competition proteins to form groups of 100 sperm into bundles that have a forward motility nearly thrice that of individual spermatozoa.

本文为此提供了证据，证据之一是单孔类动物精子的协作模式。附睾产生精子竞争蛋白，将100个精子集结成束，其向前运动性是单一精子的三倍。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

In the brain of adult rat, the positive immunohistochemical product of lSL-l (ISL-l-positive) was mainly located in the neuronal nucleus and found in discrete regions except to brain cortex, such as the Purkinje cell layer and the granular cell layer of cerebellum, the granular cell layer and the pyramidal cell layer of hippocampus, the mitral cell layer, the internal and external plexiform layer, the granular cell layer and the granular cell layer of olfactory bulb and so on, and several nuclei of the hypothalamus, midbrain and pons, such as claustrum, anterior olfactory nucleus, accumbens nucleus, caudate-ptamen, pallidum, substantia nigra, striatum, islands of Callaje, mammillary nucleus, anterior pretactal nucleus, habenular nucleus, amygdaloid nucleus, cuneate nucleus, rubral nucleus, gigantocellular reticular nucleus and so on.

在正常成年大鼠脑中，同源框基因islet-1表达产物（ISL-1）免疫组织化学阳性物质广泛分布于除大脑皮层外的神经细胞的细胞核内，ISL-1阳性神经元密集分布于小脑Purkinje细胞层和颗粒细胞层、海马的颗粒细胞层和锥体细胞层、嗅球的内丛层、外丛层、颗粒细胞层及僧帽细胞层等，另外在丘脑、中脑和桥脑的一些重要神经元核团均有分布，如，屏状核、前嗅核、伏核、尾壳核、苍白球、黑质、纹状体、Calleja岛、乳头体核、前顶盖前核、缰核、杏仁核、楔束核、红核网状巨细胞核等。

The present studies showed that two cell populations were found in haemocytes: large cell with high granularity and small cell with low granularity by flow cytometry FCM on light scanttering pattern. Two distinct cell types were identified based on phase contrast microscope: one type of cell was dark and dioptric aberration, while the other was bright and dioptric strong. By Giemsa and H.E staining, cytoplasmic staining were heterogeneous and internal particles were obvious in one type of cell, while cytoplastic staining were homogeneous and internal particles were inexistent in the other type of cell. By transmission electron microscope, we found that the mitochondria, Golgi apparatus organelles were rich and internal particles were obvious in one type of cells, and contrary to the another cells.

流式细胞术光散射图谱显示血细胞被分两类，一类为颗粒度高的大细胞，另外一类为颗粒度低的小细胞；相差显微镜观察显示，血细胞可分为胞体暗、折光性差和胞体明亮、折光性强的两类； Giemsa和H.E染色显示细胞分为胞质染色不均一、胞内颗粒明显和胞质染色均一、胞内颗粒不明显的两类；透射电镜超薄切片观察显示，颗粒明显的细胞胞质内线粒体、高尔基体等细胞器较丰富，颗粒不明显的细胞胞质内细胞器较少；负染结果表明血细胞主要分为表面不光滑、突起明显和细胞表面光滑、突起较不明显的两类。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48