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We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

The students read texts to obtain the microscope-related concepts which included the following: making water mount specimen to be observed by depending on the correct steps, the image of object shown on compound microscope was enlarged directly by the specimen; some differences of structure were found both plant cell and animal's cell, animal's cell does not have cell walls and chloroplasts, it unable to photosynthesis; small living beings of water, consumers, will eat little small living beings.

学生由阅读文章而获得的细胞相关概念主要有:能依正确的步骤,制作一个水埋法标本片进行观察,在复式显微镜下观察到的影像和实物是左右相反、上下颠倒的,植物细胞和动物细胞的形态及构造有些差异,动物细胞不具细胞壁、叶绿体,无法行光合作用,有些水中的微小生物会摄食更小的微小生物,是消费者。

Want to know, the person's skin can be divided into three-layer of cuticular, dermal, subcutaneous tissue about; All maintaining the limits that tastes generation action is in only cuticular layer, in thin thin divided in the cuticular layer that is less than one centimeter 4, quintuplicate cell, be located in the bottommest for base layer, full vivid cell can grow ceaselessly reach dissension, slowly when upgrade is advanced, the cell can squash gradually, at the same time evaporate, nutrient, until most the corneous layer outside, already was flowing do not have the corneous layer cell of life however.

要知道,人的肌肤约可分成表皮、真皮、皮下组织三层;所有保养品产生作用的范围都仅在表皮层,在薄薄不到一公分的表皮层中分了四、五层的细胞,位于最底部为基底层,布满的活细胞会不断生长及分裂、慢慢往上推进时,细胞会渐渐变扁,同时失去水分、养分,直到最外的角质层,就已是平滑却没有生命的角质层细胞了。

The method uses groovy histology and immunity to organize chemical technology, observation mars agent 6- hydroxide via celiac inject sympathetic much cling to small rat small intestine is epithelial after amine the amount of endolymph cell, cup shape cell, IgA+ oar cell, corpulent cell distributings change.

概要: 目的探究交感神经对肠道黏膜屏障的调节功能。方法采用常规组织学和免疫组织化学技术,观察经腹腔注射交感神经损毁剂6-羟多巴胺后小鼠小肠上皮内淋巴细胞、杯状细胞、IgA+浆细胞、肥大细胞的数量分布变化。

Results: It showed that esculin, demethylfuropinarine, decursin and columbianadin inhibited growth of human carcinoma cell line KB cell and their activity showed doseeffect relationship; and umbelliferone renecioate, sphondin and coumestrol inhibited growth of human leukemia cell line HL60 cell and their activity showed doseeffect relationship.

结果:马栗树皮苷、去甲基呋喃皮纳灵、前胡素和二氢山芹醇当归酸酯对人鼻咽癌细胞株KB细胞的生长有一定程度的抑制作用,且呈浓度-效应关系;伞形花内酯刘寄奴酸酯、牛防风素和考迈斯托醇对人白血病细胞株HL-60细胞的生长有一定程度的抑制作用。

Esculin, demethylfuropinarine, decursin and columbianadin inhibited growth of human carcinoma cell line KB cell. Umbelliferone renecioate, sphondin and coumestrol inhibited growth of human leukemia cell line HL60 cell.

马栗树皮苷、去甲基呋喃皮纳灵、前胡素和二氢山芹醇当归酸酯对人鼻咽癌细胞株KB细胞的生长有抑制作用;伞形花内酯刘寄奴酸酯、牛防风素和考迈斯托醇对人白血病细胞株HL-60细胞的生长有抑制作用。

Neural cell adhesion molecule L1, discovered in the 80s, is one of the matters that can promote nerve regeneration. It can mediate the interaction of cell-to-cell and cell-to-matrix, take great efforts on neurite outgrowth and fasciculation, migration of neural cells, formation of the myelin, structure of neural trace, transmembrane signal transduction, inhibiting apoptosis of neural cells, even on immune system and formation of the tumor. Li is discovered on the mouse's Cerebellar membrane primarily. Thereafter, it also is found in post- mitotic neurons, pre- and non-myelinating Schwann cells and so on.

神经细胞粘附分子L1(L1)是80年代发现的具有促进神经再生作用的物质,它能介导神经细胞与神经细胞之间的粘附,在神经轴突的生长、聚集,神经细胞的转移,纤维髓鞘的形成,神经通路的构建及信号的转导,抑制神经细胞的凋亡甚至在免疫系统、肿瘤的发生等方面均具有非常重要的作用。L1最初是从小鼠的小脑膜中发现,后来发现在有丝分裂后的神经元,髓鞘形成前及非形成髓鞘的雪旺细胞等组织中同样存在。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗;(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力;(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性;(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡;(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型,应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况,明确其靶向性;(6)将荷瘤裸鼠分3组进行放射免疫治疗,分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

FCM outcome show that osteosarcoma cell DNA become heteroploid and DNA content is abnormal. Compare with normal muscle cell, the G1 phase cell number of osteosarcoma decrease, however the S phase cell number increase significantly.

流式细胞术检测结果显示:骨肉瘤细胞DNA均呈异倍体改变,DNA含量异常,与正常肌肉细胞相比,骨肉瘤G1期细胞数减少,而S期细胞数显著增多。

Objective The origin and the nature of the Hodgkin and Reed-Sternberg cell of Hodgkins lymphoma has been attracting a lot of medical researchers engaged in studying it,for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells.Great improvement has been made since a new method of isolation of single H/RS cells from a frozen section had been set up by KUppers in 1994,and many studies have approved of the B-cell derivation of H/RS cell.lt has been reported that H/R-S cells might partly be originated from B-cell in our research before,but at the same time,we also found that only 18.8% of H/RS cells express CD20,31.3% of immunoglobulin heavy chain rearrangement have been revealed.

目的 霍奇金淋巴瘤(Hodgkins lymphoma,HL)由于它的恶性肿瘤细胞—H/RS细胞一般只占肿瘤组织的极少部分(不到1%),且散在分布在背景细胞间,因此对于H/RS细胞的来源和性质研究一直是人们探索的目标。1994年德国科隆大学Kūppers等发展了一种从冰冻组织切片上用显微操作仪挑取单个H/RS细胞的显微切割方法进行H/RS细胞基因分析后,人们对H/RS细胞来源的研究有了突破性的进展,多数支持B细胞来源。

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You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

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This is a very big challenge to developers especially that Ajax is constantly changing.

这对开发者来说是一个非常大的挑战,尤其是需要不断变化的Ajax。