查询词典 spectrophotometer

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

The optical density value of cells was detected by MTT colorimetric method, content of MDA and NO in the supernatant fluid of cultured cells was detected by spectrophotometer, and the transudatory content of LDH was detected by automatic biochemistry analyzer.

用MTT法检测细胞吸光度值，利用分光光度计分别测量细胞上清液中的MDA和NO含量。用自动生化分析仪测定培养液中LDH漏出量。

In this paper the writers have studied the diversity of contents of chlorophyll with the method of spectrophotometer in the seven breed species of malting barely in the periods of the trilobe, the jointing, the booting, the milking and the maturing.

用分光光度法研究了啤酒大麦 7个品种间叶绿素含量在三叶期、拔节期、抽穗期、灌浆期和成熟期的多样性。

Methods 100 mg of muscle of rat corpse was preprocessed before trimethylamine was extracted with anhydro-methylbenzene, then trinitrophenol was added into extracts, and yellow compounds emerged. The optical density was detected by spectrophotometer at 410 nm and the concentration of TMA-N was calculated using the standard curve. Results There was good linear correlation (R^2=0.9991, P.001) between OD and the concentration of TMA-N in the range of 1~10 mg/L.

大鼠肌肉组织样本100 mg经预处理后，将三甲胺抽提于无水甲苯中，与苦味酸作用，形成黄色的苦味酸三甲胺盐，用分光光度计在410 nm处检测吸光度，标准曲线法计算TMA-N含量。

In order to determine the contents of Vitamin C,Nitrate,and Nitrite in varied Zingiber officinale and evaluate the quality of Zingiber officinale,ultraviolet spectrophotometer is used to measure the absorbency of Zingiber officinale at special wavelength.

为评价不同处理方法对生姜中V itC、硝酸盐和亚硝酸盐含量的影响，通过紫外吸收分光光度计在特定波长下的吸收值分别对鲜姜、煮姜和泡姜进行测定。

The sizes of the self-assembly micelles of the amphiphilic block copolymers and the effects werestudied by dynamic light scattering and UV-visible spectrophotometer. The micelle sizes werelargely influenced bythe hydrophobic chain content in the copolymer, the properties of the solvent and the copolymer concentration in the organic phase. The morphology of the micelles was investigated by transmission electron microscopy. Theresults showed that the micelles appeared spheres with inner core and outer shell. The critical association concentrations were determined by pyrene monomer fluorescence probe technology. The degradability of copolymer was studied by the loss of the intrinsic viscosity in the degradation process. The results indicated that the degradation rate of PECL was slower thanthat of PELLA and PEDLLA. The stability of the copolymer micelle dispersion was examined by measuring the critical flocculation concentration, which gradually decreased with increasing the content of thehydrophobic chain in the copolymer. The rheological results showed thatthe viscosity ofthe micelle dispersion with higher content of PEG segments was firstly decreased and then increased with the increasingthe temperature. Otherwise, the variation of viscosity was irregular. The viscosity of the micelle dispersion increased with the increase of the electrolyte (Na 2SO 4) concentrations. Polymer micelles paclitaxel was prepared by self-emulsification/solvent evaporation method and solid dispersion technique with amphiphilic block polymers as the carrier material.

运用动态光散射和紫外分光光度计研究了两亲性嵌段共聚物自组装胶束的粒径及其影响因素，结果表明，自组装胶束的形成机理受制备方式的控制，胶束的粒径随着共聚物相对分子质量或疏水嵌段相对分子质量和有机相中共聚物浓度的增大而增大，随着有机溶剂的水溶性的增强而减小；采用透射电镜观察胶束的形态结构，发现胶束呈具有核壳结构的均匀球形；采用芘荧光探针法测两亲性嵌段共聚物的临界聚集浓度；通过降解过程中共聚物特性粘度的变化研究两亲性共聚物的降解性能，发现与PELLA和PEDLLA的相比，PECL 降解速率较慢；随着共聚物中疏水嵌段含量的增大，纳米分散液的稳定性逐渐下降；PEG含量高的PEDLLA纳米分散液随着温度的升高，纳米分散液的粘度先下降然后增大，反之，没有规律性，且随着电解质Na 2SO 4浓度的增大，体系粘度增大。

Finally, the properties of the drug-loaded SN have been evaluated with the help of UV spectrophotometer, and with the small molecular and water insoluble drug-sulpiride as a model drug. It is found that the entrapping efficiency of drug-loaded SN in entrapping method can amount to 96.87% and that in adsorption method is 87.63%, which show that the loading mechanism of SN is mainly adsorptive mechanism.

最后，利用紫外分光光度计，以疏水性小分子药物舒必利为模型药物，对所制备的淀粉纳米粒的载药性能进行了评价，发现淀粉纳米粒包埋载药的包封率可达到96.87％，吸附载药的包封率达到87.63％，淀粉纳米粒对舒必利的载药机理主要是吸附机理。

The contents of VC,protein,total soluble solids and gingerols in pickled gingers were determined by ultraviolet spectrophotometer.

目的：比较了不杀菌、不加防腐剂、0～4℃低温贮存和加入山梨酸钾后，在80℃水浴中杀菌15min并常温保存对泡姜口感、质地、色泽、营养成分的影响。

Methods Lung targeting gelatin microspheres of enrofloxacinwere prepared by emulsion-corsslinking method, the micorpheres were obsevred by oil lens of microscope, the diameter of the microspheres was measured and the microspheres was photoed by micro-camera. The conetnt of ENR is measured by mehtod of ultraviolet spectrophotometer.

采用乳化-化学交联法制备恩诺沙星肺靶向明胶微球，油镜下观察微球粒径大小及形态并用微型照相机（Olympus DP12-2）拍照做记录，通过紫外分光光度法检测微球中恩诺沙星的含量，进而计算得出其载药量和包封率，并通过溶出度测定法探讨其体外释放规律。

Spectrophotometer measuring, the total content of caroteinoids in saponified product was 29.22%, and in the crude extract was 5.07%.Using VC-CUSO4-H2O2 system to study its scavenging activity, the result indicated that both the saponified extract and the purified lutein could quench the hydroxyl radical.

采用抗坏血酸一CuZ十一H八一酵母体系产生的经自由基为体外模型，对制备产品清除轻自由基的能力进行研究，结果发现南瓜黄色素的皂化品和纯化碑能有效清除轻自由基，最佳的浓度为0。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新