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Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根据H5、H9亚型HA基因序列,分别设计一套特异性引物,其上、下游引物分别位於裂解位点两侧,应用RT-PCR一步法检测AIV H5和H9亚型,并验证所建立方法的敏感性;通过检测NDV、IBV和IBDV等RNA病毒及电脑软件模拟检测,验证其特异性。

I want a specific analysis of the problem.

我要一个对这问题的具体分析。

On the basis of the archetypal theories in analytical psychology rather than from any specific religion, my thesis offers a summation of the significance of Jungian archetypal images in the theory and practice of psychoanalysis.

而本文的重要工作,在于从分析心理学中的原型理论着手,不是从宗教,而是以对小说中不同人物的精神分析为基础,揭示这种原型意象在精神分析理论和实践中的重要意义和作用。

The water-contact angle is decreased with increasing the specific surface area and the content of anatase.

膜的厚度通过改变催化剂量和光利用率影响材料的亲水性。

It shows higher photocatalytic activity in the phenol degradation reaction than anatase that has very similar specific surface area.

苯酚的 光催化降解实验表明它比具有相同比表面积的锐钛矿粉体有更高的光催化活性。

The anatomical structure of a specific area or part of the body.

有一种完全环绕建筑物的某一区域的列柱法。

This new scheme suggests that a shear and/or bending deflection would be allowed to take place freely in the specific part of the anchor rod which lies between the structure and the rock mass.

此方案在结构与岩体间保持一段可以自由剪切和弯曲的锚杆段,该锚杆段的长度称为自由长度。通过对闸墙受渗透压力、闸墙自重和温度等荷载的分析,发现锚杆自由长度对锚杆轴力的影响较小,而对锚杆剪力的影响很大。

There is a very specific element and component of this, which is

他说:这里有具体的组成部分。

First, the velocity and temperature data were collected in the period of simulation using three-cup anemoscope and hygrothermograph in different heights. Then Monin-Obkhov wind and temperature vertical profile were fitted by applying least square solution. Temperatures of different time in specific point were measured. Firsthand information in land surface was collected and anthropogenic heat profile of different time was investigated.

首先,利用在不同高度上面设立的三杯风速仪和温湿度计收集了两个计算模拟时段的风速和温度资料,然后采用最小二乘法拟合得出Monin-Obkhov风温廓线;在区域内布点测量不同时段的温度值,收集地表的一些原始资料;调查区域内的不同时段的人为产热分布。

Inhibition of this pathway results in development of antigen-specific unresponsiveness and clonal anergy.

阻断该通路可以导致抗原特异性的免疫耐受和克隆不应答状态。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。