查询词典 southern

By southern blot and immunohistochemistry we find that Smad4 were efficiently knocked out in periotic vestibular cartilage capsule but not in vestibular sensory epithelium.

结果发现：1.Southern Blot及免疫组化显示小鼠内耳前庭软骨囊里Smad4被有效剔除，而前庭感觉上皮里Smad4表达没有受到明显影响。2。

As far as I can remember, the split was the three listed above and the former nominate which would become Southern Blyth's Leaf Warbler Phylloscopus reguloides reguloides .

就我能记得的，有上面列出的三种，及原先的指名亚种(将要变成 Southern Blyth's Leaf Warbler Phylloscopus reguloides reguloides )。

PCR and PCR-Southern tests showed that the antisense-PLD-γ gene has been integrated into Platanus acerifoliaWilld genome.

对抗性植株进行了PCR和PCR-Southern杂交鉴定，证明反义PLDY基因已整合进悬铃木核基因组中。

Objective This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.Methods HIV RNA, HIV DNA were detected with long-distance PCR,reverse-transcription PCR,Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells, using primers locating between long terminal repeats LTR(U5)-LTR and so on.

HIV是一种变异非常活跃的病毒，这种变异既有可能医疗增强病毒在宿主体内的适应性和生存，又有可能医疗使病毒复制受到阻碍，影响病毒在宿主体内的生存，为了更进一步掌握HIV变异规律，阐明HIV致病机理，我们用逆转录PCR、长片段PCR、Southern转印、分子杂交等对HIV感染无进展者和进展者HIV RNA、CD4细胞、HIV DNA及存在状态进行了研究。

Objective This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.Methods HIV RNA, HIV DNA were detected with long-distance PCR,reverse-transcription PCR,Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells, using primers locating between long terminal repeats LTR(U5)-LTR and so on.

HIV是一种变异非常活跃的病毒，这种变异既有可能增强病毒在宿主体内的适应性和生存，又有可能使病毒复制受到阻碍，影响病毒在宿主体内的生存，为了更进一步掌握HIV变异规律，阐明HIV致病机理，我们用逆转录PCR、长片段PCR、Southern转印、分子杂交等对HIV感染无进展者和进展者HIV RNA、CD4细胞、HIV DNA及存在状态进行了研究。

Results The Southern blot result showed that 4 among 188 goats produced by pronuclear microinjection had integrated hEPO gene.

结果在原核注射获得的188头羔羊中，经Southern blot法检测有4头羊含有hEPO基因，其中3头为母羊，1头公羊于出生后20d死亡；3头转基因母羊hEPO基因的拷贝数分别为1、10、2；Western blot检测结果显示转基因羊乳中的hEPO分子质量为32kDa；MTT法检测结果表明，在泌乳10d的3只转基因羊乳汁中，每毫升乳汁中hEPO活性分别达到1.17×102IU、1.90×104IU、1.91×104IU。

The tryptophan auxotrophs and the kanamycin resistant prototrophic revertants have been proved to contain a copy of Tn5 inserted in the chromosome by Southern Blot analysis with DIG-labeled 3. 5 kb HindⅢ fragment of pkan2 as the Tn5 probe. Four typies of prototrophic revertants could be found by the above assay.

用Tn5探针进行的Southern分子杂交证明Tn5在色氨酸营养缺陷型菌株及部分Km抗性原养回复突变型菌株中均插入在染色体DNA上，并且对菌株本身具有的内源性质粒的数目及大小没有带来影响。

By now we have reerved a large number of high quality wines from internationalized wineries and winemaking companies such as CHATEAU CHEVAL BLANC, PLAIMONT(No.3 Winemaking Company in France), MESSIAS(Top Winemaking Group in Portugal), REH KENDERMANN(Top Winemaking Group in Germany), CASA RIVAS, TAMARI(Top Winemaking Group: SOUTHERN SUN WINE GROUP in Chile), and CLOS MONTBLANC in Spain,etc.

目前我们已储备了来自法国白马酒庄CHATEAU CHEVAL BLANC、法国普莱蒙PLAIMONT酿酒集团、葡萄牙梅西亚MESSIAS公司、德国莱茵肯德曼REH KENDERMANN、智利CASA RIVAS、阿根廷 TAMARI、(智利顶级酿酒集团SOUTHERN SUN WINE GROUP)、西班牙CLOS MONT BLANC等多家国际化顶级酒庄/酿酒公司的优质葡萄酒产品，并投放国内市场。

The open reading frame of Spinacia oleracea Betaine Aldehyde Dehydrogenase was retrieved from Spinacia oleracea and inserted into the Agrobacterium tumefaciens binary vector pBin438, which was driven by CaMV35S promoter, and produced the new binary vector pBSB. A. tumefaciens LBA4404 carrying this plasmid was used in genetic transformation of plants. Forty-five primary transgenic plants were detected by PCR and verified by the Southern blotting from 65 regenerated plants, of which 27 transgenic plants had only one copy of T-DNA.

摘 要：分离出菠菜甜菜碱醛脱氢酶基因构建成由CaMV35S驱动的双元植物表达载体pBSB，农杆菌菌株LBA4404携带该载体转化棉花，获得转基因棉花植株。65株转基因植株经过PCR筛选、Southern blotting分析证明有45株为成功的转化株，外源基因已经被整合到棉花的染色体组中，并以单拷贝插入居多。

Ansochromogenes was transformed by pUC1169 from itself. 8 mutants lost ability to produce nikkomycin were screened from 4068 colonies of Vio〓 Thio〓 with Tn4560 transposition. Southern blotting suggested that Tn4560 was inserted in at least 4 positions of chromosomal DNA in S.

于39℃，MM／Vio条件下培养携带有pUC1169的7100孢子，Tn4560发生转座；筛选到4068个转座菌落，并从中得到8株尼可霉素阻断突变株；对这8株突变株的总DNA进行Southern杂交分析表明，Tn4560至少在4个不同的位点插入到7100的染色体上。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷