查询词典 southern lights

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

I n/ D The sub-species of Pterocarpus indicus from which the common kind of hungmu still is obtained, grows in Southern China and South Eastern Asia.

B！ s4 q8 v 安达曼红木的亚种生长在中国南部和东南亚，人们仍从这亚种获得普通红木。

The sub-species of Pterocarpus indicus from which the common kind of hungmu still is obtained, grows in Southern China and South Eastern Asia.

d6 w， X+ m ； g0 W 安达曼红木的亚种生长在中国南部和东南亚，人们仍从这亚种获得普通红木。

Southern corn rust , caused by Puccinia polysora Underw, is a destructive disease in maize.

南方玉米锈病是一种严重的玉米病害。

Located in southern Pudong New District, Sino-Japanese Punan Plastics Factory mainly produces plastic products.

中日浦南塑料制品厂位于浦东新区南面，主要生产塑料产品。

On the southern border of Peru, the town of Puno andsacred Lake Titicaca make for a welcome diversion.

在秘鲁的南部的边界， Puno 镇和神圣的湖 Titicaca 为受欢迎的转换做。

The buffalo's soul carries the deceased to heaven, or Puya, a long journey past the southern horrizon.

水牛的灵魂将载着死者去天堂或Puya，跨越南地平线的一段很长的旅途。

Radiolaria have played an important role in determining formation time of the ophiolites and in interpreting the complicated stratigraphy and evolutionary history of the oceanic basins before the Indian block collided with the southern margin of the Eurasian plate in early Cenozoic time.

放射虫研究在确定该区蛇绿岩的形成时代、解释造山带复杂的地层层序以及揭示印度板块与欧亚板块在古近纪碰撞之前的古海洋盆地的演化历史等方面发挥了重要作用。

According to the principles of synthesis, dominance, regionality and relative consistency, the environment degradation evaluation system is divided into three classes: vulnerable region, vulnerable type and vulnerable cell. The research area belongs to the erosion degraded Southern Red Hilly Area region. Based on the conceptions and grading, the dissertation probes into the methodology of evaluation and case study, and establishes a potential degradation evaluation system which comprises altitude, gradient, soil erosion index K, annual rainfall, the proportion of rainfall in raining period, and the drought index of rainless period.

在脆弱性概念及其分类的基础上，本文进行了脆弱性评估的方法探讨和实证研究，在脆弱性分析的基础上，选择高度、坡度、土壤可蚀性K值、降雨量、暴雨量、多雨期降雨比重和少雨期干旱指数为潜在脆弱度指标；选取作为区域人地关系、社会经济发展与管理水平综合影响结果的人类活动指标森林覆盖率和坡耕地指数作为现实脆弱度评估的人为加速侵蚀的修正指标，计算生态环境的现实脆弱度，并将其指标体系与方法用于衡阳盆地生态环境脆弱度评估的实证研究。

But this is impossible, as long as it is engaging in a market economy, there are risks in any operation.

但是，这是不可能的，只要是搞市场经济，是有风险的任何行动。

We're on the same wavelength.

我们是同道中人。