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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Comparing the run_time between insertion sort and quicksort shows insertion sort gives better performance when the given data sequence is in ascending order. For the nearly sorted list, whether insertion sort performs better than quicksort depends on the size of the list and the number of randomly ordered items. Compared to the insertion sort, the results show quicksort yields shorter run_time if the list is in descending or random order. The main drawback of quicksort is the worst case behavior of O(n2) comparisons when the list is in ascending or descending order. It has been shown that quicksort also performs poorly for nearly sorted list.

通过插入排序和快速排序执行时间比较显示:当序列以递增次序排列时,插入排序的表现比快速排序好;对于基本有序序列,插入排序算法是否比快速排序算法好取决于序列的大小和基本有序序列中随机分布元素的多少;对于递减和随机序列,快速排序的运行时间比插入排序好。

Methods: Cells which belonged to the G1-phase were sorted by Multiparameter flow cytometry according DNA diploidy in asynchronous MOLT-4 cell lines, cells were synchronized at the G1-S boundary by thymidine, and western blot analysis and multiparameter analysis of DNA versus protein content was performed to examine cyclin A、B1、D〓、Eexpression in sorted G1-phase MOLT-4 cells and synchronized by double thymidine block in G1/S phase.

以MOLT-4细胞为模型,应用流式细胞术分选出非同步化生长的G1/S转换期起始处的G1期细胞,用"双thymidine阻滞法"将细胞同步在G1/S转换期起始处的G1期,采用DNA/cyclins双参数分析法及免疫印记法,检测同步化及非同步化G1期细胞中cyclinA、B1、D〓、E的表达。

Flat pieces are sorted in the hub of a district, where the main feature is the use of a double-cross belt sorting machines for sorting operations, compared with the same cross-belt sorter postal processing center in Guangzhou, The cross-belt sorting machines seem to be smaller size, sorting of size and weight is less paper, but I have sorted the grid up to 500, the size of very alarming.

扁平件的分拣在枢纽的另外一个区,这里的主要特点是采用了一台双层的交叉带分拣机进行分拣作业,相比起同样采用交叉带分拣机的广州邮政处理中心,这里的交叉带分拣机似乎尺寸要小一些,分拣的主要是尺寸和重量较轻的文件,但是分拣的格口却多达500个,其规模非常惊人。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本,经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后,每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1:GPA-FITC单抗分选男胎脐血NRBCs;2:2号标本;3:CD36-FITC单抗分选男胎脐血NRBCs;4、5:1、3号标本;6:50个男性细胞标本;7:5个男性细胞标本;8:200ng男性DNA;9:未孕女性DNA;10:ΦX174 HaeⅢ标准,271bp:为SRY基因扩增带;383bp:为人β-珠蛋白基因扩增带内参照

Firstly, the digital watermarking was transformed randomly, and then encrypted by chaos. The encrypted watermarking was transformed to one-dimensional row vector, and the pixel value was sorted. The coefficient of primitive image of stationary wavelet transformation was expanded to one-dimensional row vector, too, and then the sorted watermarking was embedded to the sorted low frequency and turned to two dimensions.

本算法先将数字水印图像进行置乱变换,然后将置乱后的水印图像进行混沌加密,将加密后的水印按行展开成一维行向量,并将像素值从大到小排序,将原始图像平稳小波分解得到的低频系数也按行展开成一维行向量,并按从大到小排序。

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明:1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率;2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI;3分离精子的ICSI效果不低于未分离精子,但分离精子的IVF效果却低于未分离精子;4肝素能促进分离精子ICSI后的胚胎发育;5分离精子ICSI后的胚胎发育率在不同公牛之间有差异,选择能产生高质量精子的公牛进行精子分离,可以提高ICSI的效率。

Ultimately 7 out of the 7 calves produced by wapiti hinds inseminated with Y-sorted sperm were male (100%) and 8/8 calves or 100% of the calves from hinds inseminated with X-sorted sperm were female.

本研究旨在将精子专用高速的流式细胞分选技术用于梅花鹿、马鹿和山羊,初步建立了梅花鹿、马鹿和山羊分选精液的低剂量授精技术体系和进一步对高纯度分选奶牛精液采用扣除杂交筛选,初步获得牛XY精子间的差异表达基因。

A low complexity detection scheme for the MIMO system with distributed transmit antennas is presented. Based on a Sorted CHolesky Decomposition, the additive gaussian color noise after match filter is whitened; The detection sequence according to the post signal noise ratio is reordered, and the risk of error propagation is degraded. The whole algorithm only requires one matrix inverse.

摘要针对发射天线分布的MIMO信号,基于排序的乔列斯基分解过程(Sorted CHolesky Decomposition,SCHD),将匹配滤波后的加性高斯色噪声转化成加性高斯白噪声,根据后验信噪比对发射信号检测顺序进行重排,降低了误差传播的影响,该算法仅需一次矩阵求逆操作。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。