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With the inducing time prolonged, the amount of fusion protein increased, and the highest content amounted to 39. 14% of total protein. Different temperature (15℃, 25℃ and 37℃) could induce expression of fusion protein, and the higher inducing temperature was, the more total fusion protein and soluble fusion protein was produced. Most fusion protein was expressed in the cytoplasm and was soluble, a little of fusion protein was expressed in periplasm or expressed as inclusion bodies, and no fusion protein was found in culture. Furthermore, the results of in vitro enzymatic reaction suggested that the fusion protein could catalyze the methylation of theobromine.

随着诱导时间的延长，表达的融合蛋白产量逐渐增加，表达蛋白总量最高可达到菌体总蛋白的39.14％；在15℃、25℃和37℃三个不同的诱导温度下，均能诱导产生融合蛋白，而且产生的融合蛋白的总量及其可溶性部分所占比例均随诱导温度升高而增加；在菌体的各个部位中，融合蛋白主要在细胞质中以可溶的形式进行表达，有少量在外周质中表达或以包含体形式在细胞质中表达。

The most of the product became soluble from inclusion body by lowering induction temperature. At the same time the gene was cloned into vector pMAL-p2X, which was translated in E. coli TB1 and expressed a fusion protein. We optimized the induction time and achieved high expression. But there was no obvious product secreted in the periplasm extract. Almost all fusion protein is expressed soluble in cytoplasm.

利用融合型表达载体pTYB11-EFE融合型表达，对诱导剂IPTG的浓度和诱导温度进行了优化，通过降低诱导温度使产物由包含体变成可溶性融合型产物；利用融合型分泌表达载体pMAL-p2X构建了表达载体pMAL-p2X-EFE-7~#，对诱导时间进行了优化，获得了较高的表达量，但发现产物没有明显的分泌表达，绝大部分是以可溶形式存在于胞内。

Grapevine; Plasmopara viticola; POD ; Soluble protein ; SOD ; Soluble sugars

齐慧霞；刘永军；吴学仁；齐永顺；李双民

The short time oxidized product is insoluble in common organic solvents and water, but the water-soluble derivatives were obtained after hydrolysis under a basic condition. It′s IR spectrum is similar with that of C60 polyhydric derivatives. The extensive oxidation product of C60 is water-soluble without any further dispose.

一种产物在有机溶剂及水中均不溶，但经碱性水解后得到水溶性产物，其红外光谱与C60羟基化合物相似；在长时间通O3条件下，得到水溶性的C60氧化产物。

MethodsIn the solvent of 1 mol/L NaOH,using the reaction of the total components of water-soluble phenolic acidity in Danshen and the NaNO2-Al(NO3)3,the total components of water-soluble phenolic acidity in compound Danshen preparation(calculated with protocatechualdehyde )were determined by colorimetric spectrophotometry.

方法利用丹参中水溶性酚酸类成分与NaNO2-Al(NO3)3在碱性条件下的显色反应，比色法测定复方丹参制剂中水溶性酚酸的总量。

MethodsIn the solvent of 1 mol/L NaOH, using the reaction of the water-soluble phenolic acidity in Radix Salviae Miltiorrhizae and the NaNO_2-Al(NO_3)_3, the total components of water-soluble phenolic acidity in Radix Salviae Miltiorrhizae injections and drip solution(calculated with protocatechualdehyde) were determined by colorimetric s...

方法利用丹参中水溶性酚酸类成分与亚硝酸钠和硝酸铝溶液在碱性条件下的显色反应，比色法测定该两种制剂中水溶性酚酸的总量。结果原儿茶醛在3.336~14.46mg/L范围内呈良好的线性关系，回归方程为：A=0.02777C+0.01279(r=0.9996)，加样平均回收率为100.8%，RSD为0.96%。结论该法简便快速，结果准确，重现性好，可用于该制剂的质量控制。

Oncidium on 15 hrs light period and night break treatments exhibited heavier fresh and dry weights, lower total soluble sugar content in pseudobulb because nutrient translocated for inflorescence development, however water content was no significantly different. After treatment of 9 hrs and 12 hrs light period, the total soluble sugar content in pseudobulb of Oncidium was higher than other treatments because of consuming less nutrient transfered from pseudobulb due to poor inflorescence development and the quality of inflorescence also showed worse.

以15小时电照周期处理与暗期中断处理之植株乾鲜重较重，含水量则无明显差异，而假球茎内的全可溶性糖含量则较其他处理组低，因为花序发育时，假球茎内的碳水化合物转移到花序，以提供花序发育所需之养分。9小时电照周期处理组与12小时电照周期处理组方面，因为其花序品质较差，消耗的养分较少，故其假球茎内的全可溶性糖含量较高。

The water-soluble, 650ml/L edible alcohol and 950ml/L edible alcohol-soluble extracts of wild Psidium guajava leaves in Panzhihua area may have different hypoglycemic potential.

攀枝花地区野生番石榴叶的水提取物、650ml/L和950ml/L食用乙醇提取物具有不同程度的降血糖作用。

Those skilled in the art will be aware of the relationship between saponi ns and their sapogenins, and that the latter tend to be fat-soluble whereas the s aponins tend to be water-soluble.

专业的药剂师应该注意到皂甙与相应的皂甙配基的联系，皂甙配基一般显示亲油溶性而皂甙一般显示亲水溶性，因此皂甙配基更容易穿过血液大脑屏蔽。

The results showed that the fractions of alcohol-benzene soluble and water soluble decreased with the process of decomposition. The concentration of semicellulose and cellulose increased firstly and then decreased. However, the concentration of lignin increased.

结果显示，随着腐解进行，腐解产物中的苯–醇溶性、水溶性组分下降，半纤维素和纤维素含量先上升后下降，而木质素增加；腐解物的能态呈现上下起伏、下降和相对稳定3个阶段的变化，总体是一个放能过程。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。