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Purpose In the present study, the protective effect of SOD on the paraquat -induced inflammatory injury in hamster cheek pouch mucosa was assessed. Furthermore. a series of experiments were performed to study the mechanisms of SOD anti-inflammatory action in this study.

(1)通过常规组织病理学观察、图像分析系统等技术,评价SOD防治金黄地鼠口腔粘膜炎性损害的效果;(2)应用电子自旋共振、免疫组织化学、放射免疫法等技术,探讨SOD可能的抗炎机理。

The granulation index, MDA and activity of SOD in granulated cells were larger than in non-granulated cells; similar to POD's, SOD activity also reduced, but SOD activity in granulated cells of apomictic fruit reached bottom earlier than in non-granulated cells; GSH and ASA of non-granulated cells were higher than those of granulated cells; granulation of Citrus guanxi-miyou might related to disorder of active oxygen metabolism

SOD活性的变化趋势与POD活性相似,但自交果粒化汁胞SOD活性出现最低点时间早于未粒化汁胞;未粒化汁胞GSH 、AsA含量大于粒化汁胞。琯溪蜜柚果实汁胞粒化可能和汁胞的活性氧代谢失调有关。

Lycopersicon esculentum var.cerasiforme plants were planted in garden pots and 2 000 mg·L-1 SOD were sprayed on them one,two,three times respectively,the ck was sprayed by water,the effects of extrinsic SOD on anti-oxidase activity and the contents of MDA and sugar of the tomatoes were studied.

&SOD水果&是近几年来食品消费领域的一个价格昂贵的新产品[1~5]。根据&自由基启动衰老&的理论[6],由于SOD在清除氧自由基中的重要作用,使消费者相信富含活性SOD的食品可以清除体内的氧自由基,起到延缓衰老的作用。

Nigrum and S. melongena seedlings presented a different rule. The leaf SOD activities of S. nigrum increased under low Cd (≤50 μM) and then decreased, POD and CAT increased continuously. However, SOD activities of S. melongena gradually decreased, POD activities increased and the CAT presented the rule of drop-rise-drop. SOD, POD, and CAT in the leaves of S. nigrum plaved an important role against Cd stress.

龙葵叶片SOD活性随镉胁迫程度的增加先增高后降低,POD和CAT活性基本上表现为逐渐上升趋势,而茄子叶片中SOD活性逐渐下降,POD逐渐上升,CAT呈先降后升又降的变化规律,龙葵体内的3种抗氧化酶对镉胁迫起到较强的抗氧化保护作用。

It's founded that Pistia stratiotes' soluble protein content,proline,peroxidase and catalase increased first and decreased afterwards with the augmenting of Cd2+ and Cr6+ concentration in the solution;With Cd2+ and Cr6+ concentration augmenting,SOD activity of Pistia Stratiotes increased first and decreased afterwards;But with Cd2+ Concentration augmenting, SOD Activity of Myriophyllum spicatum increased first and decreased afterwards; with Cr6+ concentration augmenting,SOD activity Myriophyllum spicatum droped at all times.

结果表明:随着水体Cd2+和Cr6+浓度的增加,大薸和穗状狐尾藻的可溶性蛋白含量、脯氨酸、叶绿素、丙二醛含量、硝酸还原酶活性、过氧化物酶活性、过氧化氢酶活性都是先升高后降低;随着Cd2+和Cr6+浓度的增加大薸超氧化物歧化酶活性先升后降;而穗状狐尾藻随着Cd2+浓度的增加SOD活性先升后降,随着Cr6+浓度的增加,SOD活性直线下降。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示:(1)SOD活性在52.98~133.20 U·g-1·h-1之间,并在早期单胚形成后一直保持上升的趋势,表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关;(2)POD活性在0.05~0.50 U·mg-1·min-1之间,呈现出先下降后升高的趋势,在胚性愈伤组织中最高,而在后期单胚形成时降至最低,表明其与原胚团的分裂和分化呈正相关,但与PEMⅢ向后期单胚的发育呈负相关;(3)CAT活性在0.86~2.81 U·mg-1·min-1之间,表现出升-降-升的变化趋势,在早期单胚形成时升至最高,在早期子叶胚形成时降至最低,表明其与早期单胚的发育呈正相关,而与中期单胚和早期子叶胚的发育呈负相关。

The results showed that spermatozoal SOD activity in infertile group (103±39 U/10 ̄9cells), was significantly lower than that in fertile group (144±37 U/109 cells), but no significant difference was observed in seminal plasma SOD activity between the groups.

经相关分析,精子SOD活性与精子活动率、精子正常形态及精子尾部低渗肿胀率间均呈显著性正相关;而精浆SOD活性与上述三参数、精子密度及精子SOD活性间均无显著相关性。

The rule of growth and decline about main biochemical material (solubility protein, solubility sugar, SOD and gossypol IAA) contacting with stomatic embryogenesis showed that content of solubility protein and SOD activity was differed in the different period, being lower in earlier callus and reaching maximum in 120 day.

对陆地棉愈伤组织诱导及体细胞胚发生过程中的主要生化物质(可溶性蛋白含量、可溶性糖含量、SOD活性和IAA氧化酶活性)的测定表明,各个时期的可溶性蛋白含量、SOD活性是不同的,在愈伤组织形成初期较低,随着愈伤组织的生长及胚状体的形成,可溶性蛋白含量、SOD活性逐渐上升,在120d时达最大值;而可溶性糖含量在60d时有一个高峰值,在120d时达到最低值;IAA氧化酶活性在60d、120d时酶活较高,90d的酶活较低。

The relationship between ageing and the level of NO, SOD and MDA in hear and brain of aged ratsSONG Chun mei,MA Hong bo,WANG Chang wen,LI Yong jin(Department of Nutrition, Jilin Medical College, Jilin City, Jilin Province,132013,China)Abstract:ObjectiveTo determine the contents of NO, MDA and the activity of SOD in heart and brain of aged rats, and probe the relationship between ageing and the level of NO, SOD and MDA.

目的测定大鼠心脏和脑组织中一氧化氮、丙二醛含量和超氧化物歧化酶活性,探讨NO、SOD和MDA与衰老的关系,为延缓衰老、合理而又科学地应用抗衰老食品和保健品、提高机体SOD活性和降低MDA的含量、减少老年性疾病的发生提供理论依据。

Except for female pupas, SOD activities of progenies of the hybrids in different developmental stages were significantly higher than that of progenies of self-cross.

杂交后代的各虫态,除雌蛹外其SOD活性均高于其自交后代的SOD活性;以黄色型黄粉虫为母本的雌、雄成虫和雄蛹的SOD活性较高,而以黑色型黄粉虫为母本的雌蛹和幼虫的SOD活性较高。

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