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24 New Zealand white rabbits were divided into control group,Saline group and Mailuoning group,on the latter two groups the models of Hemifacial spasm were made by the temporal superficial artery closely contacting the main trunk of facial nerve at stylomastoid foramen.From the 5th week,the Saline and Mailuoning were injected intravenously into ear margin for 2 weeks on Saline and Mailuoning group respectively.At the 7th week,the MDA and SOD in serum were measured,meawhile the microstructure and ultrastructure of facial nerve were observed on 3 animal groups.

将24只新西兰大白兔随机分为对照组、脉络宁组和生理盐水组,在手术显微镜下分离出其茎乳孔处的面神经主干和颞浅动脉,使二者密切接触,以制备半面痉挛动物模型;术后第5周起对脉络宁组和生理盐水组分别自耳缘静脉推注脉络宁注射液和生理盐水持续2周;第7周取3组动物血清测定丙二醛和超氧化物歧化酶,并处死取材行面神经光、电镜观察。

HUANG Jian,ZHUO Lianshi,WANG Yongyu(College of Traditional Chinese Medicine of Chongqing Medical University,401331,China)Abstract:ObjectiveTo observe the effects of massage on synovia SOD,MDA and NO contents in rabbits with Knee Osteoarthritis.

目的观察推拿加功能训练对膝骨关节炎家兔模型关节液中超氧化物歧化酶、丙二醛和一氧化氮的影响,探讨推拿加功能训练治疗膝骨关节炎的机制。

At the same time the general activity of SOD and the content of MDA and TChE in mice brain's tissue was measured with UV.

同时,利用紫外分光光度法,测定脑组织内总超氧化物歧化酶的活力、丙二醛的含量、乙酰胆碱酯酶的含量。

After gestational periods the activities of SOD,MDA,GSH-Px,TChE and CAT in brain were analyzed respectively.

采用饮水染毒,染毒时间为21d,分娩后每窝随机选取6只乳鼠测定脑匀浆超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、乙酰胆碱酯酶活性和丙二醛的含量。

Then killed the animals and extracted hippocampus.The serum of TChE,SOD CAT and MDA were detected.

然后将各组剩余小鼠断髓处死,冰台上迅速取出脑组织,在预冷的生理盐水中漂洗,除去血液,分离出两侧的海马,投入4℃冰浴的玻璃匀浆器中,按1∶9于生理盐水中制成10%脑组织匀浆,在4℃下4000r/min离心10min,取上清液以检测小鼠海马组织中乙酰胆碱酯酶、超氧化物歧化酶、过氧化氢酶的活力和丙二醛的含量。

The serum levels of TChE and SOD and CAT in hippocampus of mice in Cold group was also significantly higher than those in the Control group and Warm group(P 0.05或P 0.01),while the serum level of MDA in the Control group and Warm group were higher than that in the Cold group(P 0.05或P 0.01). Conclusion:(1)Long proper winter swimming could improve learning and memory abilities of mice.(2) Winter swimming could improve the ability of learning and memory by coordinate the content of acetylcholinesterase (3) This effect may be related to the decreased activity of TChE and the increased activity of anti-oxidatio.

1长期适量的冬泳运动可以促进小鼠学习记忆能力。2冬泳运动可以通过增加小鼠脑内海马组织中ACh的含量而影响TChE的活力。3冬泳运动促进学习记忆的机制可能是:3.1冬泳运动通过冷应激引起的神经内分泌系统的反应,来改变中枢神经系统的功能,使小鼠脑内乙酰胆碱的代谢增强、含量增加,从而促进学习记忆能力。3.2冬泳运动通过增强机体清除自由基的能力,来减轻脂质过氧化的程度,对小鼠的脑损伤起到保护作用,从而促进学习记忆能力。

The Morris water maze task and step down test were used to test the learning ability of mice, and the examination index includes protein and MDA content of brain, SOD and TChE activity.

Morris水迷宫实验显示:不同剂量强化组动物的逃避潜伏期缩短(P<0.05),在目标象限的游泳时间和路程显著增加(P<0.05),穿越目标次数也增多(P<0.05)。

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时,应用形态学观察法,研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化;应用免疫荧光细胞化学和蛋白质印迹分析的方法,研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达;应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化;应用交配试验,检测雄鼠受精能力及其致孕雌鼠的产仔数的变化,用以揭示热应激对雄鼠生殖机能的影响。

The results showed that 22.5μL/L of clove leaf oil had the best preservation effect on peach. After 35 days of stock, the sound fruit rate was 94%, 46.9% higher than compared group. The rotten index and the browning index were 94.1% and 85.2% lower than the control, respectivly, and the treatment could remarkablely reduce the respiration rate and ethylene release, and postponed ethylene release peak and respiration peak 5 and 10d, respectively in comparison with CK, it also delayed the drop of firmness and contents of titrable acids of the peach. When stored for 35d, firmness of control group and 22.5μL/L of clove leaf oil were decreased 60% and 34% compared with initial storage, and the activities of SOD, CAT and POD were enhanced, but the contents of SSC had no obvious difference with control.

结果表明,与对照相比,22.5μL/L丁香叶油处理对桃果实的防腐保鲜效果最好,贮藏35d时好果率为94%,比对照提高了46.9%,腐烂指数和褐变指数分别比对照低94.1%和85.2%;丁香叶油处理可以有效降低桃果实的呼吸强度和乙烯释放量,呼吸强度高峰和乙烯释放量高峰分别推迟5和10d,延缓了果实硬度和可滴定酸含量下降的速度;贮藏35d时对照和22.5μL/L丁香叶油处理果实硬度分别比贮藏初期降低了60%和34%;丁香叶油处理提高了超氧化物歧化酶、过氧化氢酶、过氧化物酶3种膜脂过氧化物酶的活性,但可溶性固形物含量与对照相比无明显差异。

This paper summarizes the research progress on the family of antioxidant enzymes in trematodes including glutathione peroxidase, superoxide dismutase and peroxiredoxin in the past decades.

本文综述近年来对医学吸虫谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)、超氧化物歧化酶(superoxide dismutase,SOD)及抗氧化蛋白(peroxire-doxin,PRx)等抗氧化酶家族的研究进展。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。