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L^(-1) VP-16 for 6, 12, 24 and 48 h. Meanwhile or 24 h later, 316 mg?L^(-1) SOD was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of Hela cells were determined. Results The production of ROS and the apoptosis of Hella cells increased as the action of DDP and ADM continued within 24 h, hut VP-16 failed to produce such effects.

宫颈癌Hela细胞经顺铂、足叶乙甙(VP-16)和阿霉素血浆峰浓度不同时间作用后,测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化,并测定不同时间加入SOD对DDP、ADM、VP-16诱导Hela细胞凋亡及p53、c-fos基因表达的影响。

Results: Compared with I/R+insulin group and I/R+pioglitazone group. the ultrastructure of cardiomyocytes suffering ischemia reperfusion injury recovered markedly, the fluorescence intensity of the intracellular calcium ion decreased, the survival rate of cardiomyocytes increased, LDH, CK, MDA concentration in culture medium decreased and SOD concentration in culture medium increased in I/R+insulin+pioglitazone group.

结果:吡格列酮与胰岛素联合应用可提高缺血再灌注损伤心肌细胞的存活率,降低细胞培养液中的LDH、CK和MDA含量,提高SOD的含量,降低心肌细胞钙离子染色的荧光强度,部分恢复心肌细胞的超微结构,与单独应用吡格列酮或胰岛素相比,具有统计学意义。

LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochchondral respiration were evaluated in each group; apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis; the immuneohistochemical method was used to determine the expression of bcl-2 and Caspase-3 ; the morphoiogic changes were observed with microscope.

将培养的L-6TG大鼠肌母细胞随机分为3组:①正常对照组,②缺血再灌注组(1R组),③缺血预处理组,观测了培养上清中LDH、细胞内SOD、XOD、Ca(上标 2+)含量的变化;采用MTT法检测线粒体的功能;利用流式细胞仪和细胞DNA电泳结果检测细胞凋亡情况;采用免疫组织化学的方法检测bcl-2及Caspase-3的蛋白表达情况,结合自动图像分析系统对其结果进行定量分析;在光镜下观察细胞的形态学改变。

The seed vigor, SOD and POD decreased significantly;and electric conductivity, the content of soluble sugar and MDA increased significantly.

而超干处理的种子贮藏18个月后,种子的发芽率、发芽指数、活力指数、电导率、可溶性糖含量、SOD活性、POD活性和MDA含量均无显著变化。

The results showed that with the immersion time of straw and straw leaching solution concentration increased, the germination rate of wheat seed decreases, the time of emergence of wheat seed also will extend the POD activity of wheat seedlings, SOD activity and root activity show a downward trend , leaf MDA content was increased.

结果表明,随着秸秆浸泡时间的延长和秸秆浸出液浓度的增加,小麦种子的萌发率随之降低,小麦种子的出苗时间也随之延长,小麦幼苗POD活性增加,SOD活性和根系活力呈现下降趋势,叶片MDA含量却升高。

The total SOD activity of an unknown sample could be obtained by interpolating its reading to the standard curve.

总SOD的一个未知样品可插读它的标准曲线。

Effects of different concentrations of exogenous SOD on antioxidant enzyme activities and sugar contents in leaf and fruit were studied, taking tetraploid plant in Vitis vinifera cv 'Muscat humburg' as materials and water treatment as the CK.

以四倍体玫瑰香葡萄为试材,以喷清水为对照,探讨了不同浓度外源SOD对叶片、果实抗氧化酶活性和含糖量的影响。

Low dose Yiweitang group; 4. Diethylstilbestrol group; 5. Model control group. After 4 weeks intervention, get the left ovarie, a part of hypothalami and pituitary gland from head-off rat, and observe expression of ER, FSHR and GnRH by immune histochemistry method . Then extract RNA from the rihgt ovarie and the rest hypothalami and pituitary gland, so as to measure the expression of GnRHmRNA 、 ERmRNA 、 FSHRmRNA and P 16mRNA by RT-PCR. And the cerebrum were made to homogenate to examine SOD, GSH-PX and MDA.

各组实验动物灌药4周后,断头处死,取部分下丘脑、垂体及左侧卵巢组织固定切片,免疫组化法检测 ER 和 FSHR 表达、 GnRH ;取剩余部分下丘脑、垂体及右侧卵巢组织提取总 RNA ,用于 RT-PCR 检测下丘脑 GnRHmRNA ,下丘脑、垂体的 P 16mRNA 及下丘脑、垂体、卵巢的 ERmRNA 、 FSHRmRNA 基因表达;另外取部分大脑组织制作脑组织匀浆,用于 SOD 和 GSH-PX 酶活力以及 MDA 含量的测定。

It is Investigated that the effects of vitamin C and E on M. nipponense in different molting stages by measuring oxygen consumption rates, ammonia-N excretion rates and activities of antioxidant enzymes(SOD,CAT,GSH-Px,GST,GR) in three groups which are control group,Vc group andgroup.1. The oxygen consumption rates are high in stages D2 and A, but low in stage C.

设立三个试验组:对照组,V_C组(200mgV_C/kg)和V_C+V_E组(200mgV_C/kg+400mg V_E/kg),以上述分期结果为标准,测定了三个试验组的日本沼虾在蜕皮各时期的耗氧率,排氨率以及抗氧化酶(超氧化物歧化酶SOD,过氧化氢酶CAT,谷胱甘肽过氧化物酶GSH-P_X,谷胱甘肽转移酶GST,谷胱甘肽还原酶GR)活性的变化,结果如下: 1。

With one-year-old dormant branches of four sweet cherry varieties as test materials, the activities of SOD and POD in different periods were measured.

方法]以4个甜樱桃品种1年生枝条为试材,测定不同时期的SOD、POD酶活性变化。

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