查询词典 sod-forming

SOD is existed in any aerobiccell. According to the metalion contained, SOD is devided into four types: Cu/Zn-SOD, Mn-SOD,Fe-SOD and Ni-SOD. Among the total, SOD is studyed more. SOD can catalyze·O_2~- to take place dismutation reaction.

人们发现所有需氧细胞中都存在SOD，按照所含金属离子的不同，SOD可分为四种类型，即：Cu／Zn-SOD、Mn-SOD、Fe-SOD和Ni-SOD，其中Cu／Zn-SOD研究的最多。

objective to study gastrodia elata blume to effect tau protein,sod,mda expression in gyrus hippocampi and cerebral cortex of experimental mice.methods to inject okadaic acid to mice ventriculus lateralis and to measure tau protein level,sod activity and lipid superoxide mda content of sod control group,oa injection group,oa and gastiodia elata injection solution group.results tau protein of experimental group(p＜0.05/p＞0.05),sod was lower than model group(p＜0.001) and was higher than control group(p＜0.05).conclusion gastrodia elata blume can increase sod activity and reduce tau protein expression and superoxide lipid forming in brain tissue of experimental dementia mice caused by oa.it can prevent and treat ad.

目的 观察天麻对痴呆模型大鼠海马、皮质神经元微管相关蛋白、超氧化物歧化酶和脂质过氧化物丙二醛表达的影响，探讨其治疗阿尔茨海默病(alzheimer disease，ad)的作用机制。方法用冈田酸(okadaic acid，oa)注射大鼠侧脑室造模，测定模型组、实验组、对照组海马和皮质tau蛋白、sod、mda的含量。结果实验组tau蛋白低于模型组(p＜0.001)，高于对照组(p＜0.05)；sod高于模型组(p＜0.001)和对照组(p＜0.05)；mda低于模型组(p＜0.001)，高于对照组(p＜0.05)。结论天麻可增强oa致实验性痴呆大鼠脑组织sod活性，降低mda蓄积和tau蛋白生成，具有防治阿尔茨海默病的作用。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

12H treatment group compared with 12h model group: the level of SOD, MDA of the Gardenoside group is no different with the model group; the level of the SOD of the Baical in group increase and the level of MDA is no different; the level of SOD of the Concha Margatitifera Usta is no different and the MDA of it decreased than the model; the level of SOD of the Cholic Acid group increased and the level of MDA decreased; the level of SOD of the Hefang Group increased and the level of MDA of it decreased than the model group.

考虑nNos可能只是参与缺血早期的神经毒性作用。到缺血24小时这一时程已经减低或耗竭。大鼠局灶性脑缺血12小时模型组缺血脑组织P53光密度值含量较正常组明显增高（p＜0.01）。珍珠母治疗脑缺血12小时组缺血脑组织P53光密度值较缺血12小时模型组明显降低。胆酸治疗脑缺血12小时组缺血脑组织P53光密度值较缺血12小时模型组明显降低（p＜0.05）。栀子苷治疗脑缺血12小时组缺血脑组织P53光密度值较缺血12小时模型组明显降低（p＜0.05）。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

Active oxygen is found to be involved in apoptotic progress affected by exogenous SOD and measurement of SOD in cell sap. Arsenic compounds cause a decrease of SOD in cell. It is exactly the decrease that make plenty of active oxygen gather in mitochondria, and starts the apoptotic progress. The analysis of SOD values shows that active oxygen has an effect on apoptosis.

通过加入外源性SOD和对胞液SOD的测量，发现胂化物引起细胞凋亡的过程，有活性氧的参与，并且胂化物还导致细胞内SOD值的减少，正是由于这种降低，才导致了细胞内特别是线粒体中活性氧大量聚集，启动了细胞凋亡过程，通过对SOD值的分析，证明了活性氧在细胞凋亡中的作用。

SOD group was significantly better than U74389G group in coefficient of lung, SOD activity and apoptosis index,（P .01,P .05）. U74389G group was significantly better than SOD group in contents of MDA,(P .01), and there was no significant difference between U74389G group and SOD group in RAC.

SOD组在缓解肺系数、降低SOD活性及抑制凋亡方面优于U74389G组（P 。01，P 。05）；U74389G组在抑制MDA含量方面优于SOD组（P 。01），而两组间RAC差异无显著性。

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RESULTS The SOD activity had linear correlation with pH in the range of pH 2.2-8.0 when SOD concentration was fixed. The varication of activity after ultrosonication had linear correlcation with fluoresent intensity at 340 nm. With increase of uhtrosonic frequency, the microenviroment of tyrosine and tryptophan inside the SOD molecule was changed, then the balance of hydrophokicity and hydrophilicity inside the molecule was broken, this induced the blue shift of synchronous spectrum band and decrease of SOD activity.

结果 SOD浓度一定、pH2.2~8.0时，活性与其线性相关，超声后活性差值与340 nm荧光强度差值的线性相关；随着超声次数的增加，蛋白质SOD分子内部的酪氨酸、色氨酸所处的微环境被扰动，打破了分子内部的疏水和亲水平衡，使蛋白质的活性降低，在荧光检测中观察到普通荧光谱带蓝移，同步荧光的荧光峰位总体呈现峰位蓝移和强度下降。

The electrophoresis patterns of EST and SOD had a tissue-specific distribution. The Sod should consist of two loci, which code dimeric s-SOD and tetrameric m-SOD respectively.

结果表明，二色裂江珧组织内的EST和SOD存在不同程度的组织特异性，EST共检测出15条酶带，SOD检测出8条酶带，且两种同工酶在消化盲囊中活性最高。

Let that vacation time mellowness carry over intothe New Year.

让假期的愉悦延续到新年。

At present, many scholars agree with the views of Schultz et al.

目前，我国的很多学者都基本上认同舒尔茨等人的观点。