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The SOD and the LDH-1 activities and EST (R_f=0.17 and R_f=0.72) at 1℃weresignificantly higher than the values of the room temperature(P<0.05), but the total enzymeactivities of LDH, G6PDH and EST at room temperature and 1℃were similar(P>0.05).This showed well-developed anoxia tolerance of the Heilongjiang wood frog to maintainplasma viability when low temperature interrupts the delivery of oxygen and substrates. LDH-1rose significantly avoided the liver lactic acid accumulation that may created the liver to bewearied. EST (R_f=0.17 and R_f=0.72) rose may enhance the ester metabolism to offer muchenergy. These showed steady metabolic rate and relative stable organism environment of theHeilongjiang wood frog to survive under low temperature.Native PAGE separated proteins of liver tissue successfully on the better condition is 6%final gel concentration and there were 15~19 bands identified on this condition.

同功酶电泳图谱显示,1℃组与室温组黑龙江林蛙肝脏LDH酶活性差异不显著,均以LDH-5为主,但1℃组LDH-1显著高于室温组(P<0.05),推测黑龙江林蛙为适应低温低氧环境,增强LDH-1酶活性以较好的利用乳酸,避免因大量乳酸形成造成肝脏疲劳;而1℃组肝脏G6PDH酶活性无显著变化,进一步说明低温条件下葡萄糖戊糖途径和糖酵解作用的互相协调,维持机体代谢的稳定;1℃组和室温组EST总酶比活力无显著变化,但1℃组中R_f=0.17和R_f=0.72的EST同工酶显著升高(P<0.05),推测在低温条件下黑龙江林蛙可能通过选择性增强部分酯酶同功酶活性,增加肝脏酯类物质代谢,为机体提供维持生命必需的最低能量。

The 5 min-fomation of superoxide anions was used to determine the activity of xanthine oxidaseNitro Blue Btetra...

再将20、40、60μg/ml renierol或100 U/ml阳性对照SOD加入25℃预热过的邻苯三酚,在波长420 nm处测定吸光值,观察renierol对自由基的直接清除作用。

The methods of xanthine oxidase and Southern blotting were used to determine the activity of superoxide dismutase and the length of telomere.

用黄嘌呤氧化酶法对肝组织SOD活性进行测定,用Southern blotting方法测定肝组织端粒长度。

Using the artificial water control technique in the shed of freerain and periodically changing water supply at different growth stages during annual growth period, we examined the SOD activity in leaves of %Acer truncatum% youngling to water stress .

通过旱棚人工控水,以元宝枫的生长阶段作为因素,采用三元二次通用旋转组合设计,对元宝枫不同生长阶段下供水量与供水方式对叶片中超氧化物歧化酶活性的影响进行了研究。

OBJECTIVE:To obtain high purified and cheap human copper/zine superoxide dismutase with genetic engineering and affinity chromatography.

目的:用基因工程的方法获得人铜锌超氧化物歧化酶的高产菌,通过发酵培养,最后纯化获得大量廉价的高纯度的hCu,Zn-SOD。

The activities of peroxidases and superoxide dismutase increased dramatically 2 h after being treated by avermectin dilutions which were added with copper abietate, 1427 and 412103. This implied that the three adjuvants had higher permeabilities on P. xylostella larvae.

阿维菌素药液分别加人松香酸铜、1427和412103后使试虫体内POD和SOD的活性明显提高,从侧面说明这3种助剂对虫体更具渗透性。

MethodsAfter acate liver injury model was induced by carbon tetrachloride (CCl4), mice were administrated intragastrically with Hypericum japonicum.The serum ALT, AST, SOD, NO, TNF-α, IL-6 were determined and pathological change of liver was observed.

方法以四氯化碳(CCl4)制备小鼠急性肝损伤的模型,田基黄水煎剂灌胃,检测小鼠血清中的丙氨酸氨基转移酶、天冬氨酸氨基转移酶、超氧化物歧化酶活力以及一氧化氮、肿瘤坏死因子-α、白介素-6的含量,观察肝组织的病理损伤程度。

The effects of cryopreservation (-196℃) on the antioxidant activities of enzymes (SOD, CAT, GSH-PX and GR) in seminal plasma and spermatozoa of Russian sturgeon Acipenser gueldenstaedti were studied by a reagent kit to clarify damage mechanism of spermatozoa.

为了探讨超低温(-196℃)对精子的损伤机理,采用试剂盒测定了冷冻前后俄罗斯鲟精浆和精子中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷肽甘肽还原酶等酶活性。

The results showed that either of the extracted propolis had significant inhibitory effect on the level of triglyceride, total cholesterol, LDL-C,GPT and GOT in serum, and TC, TG and MDA in liver, but were ineffective on HDL-C, MDA, SOD and NO levels in serum. Ethanol extracted propolis also reduced the body weight, liver weight and liver index of hyperlipidemic SD rats, while water extracted propolis were adiaphorous.

结果表明:蜂胶醇提液和水提液均能有效抑制高脂血症SD大鼠血清中的甘油三酯、总胆固醇、低密度脂蛋白、谷丙转氨酶、谷草转氨酶的升高,并且能有效抑制肝脏组织中的TC、TG、MDA的升高,但对血清中的高密度脂蛋白、超氧化物歧化酶、丙二醛、一氧化氮的影响不明显;蜂胶醇提液能有效降低高脂血症SD大鼠的体重、肝重及肝指数,蜂胶水提液对体重、肝重及肝指数的影响则不显著。

Eight Alnus cremastogyne clones, which simulated with different concentration of sodium chloride, were measured with four physiological indexes, including SOD activity, MDA content, chlorophyll content and soluble proteins content.

以不同质量分数的盐胁迫对桤木Alnus cremastogyne 8个无性系,在盆栽条件下测定叶片中丙二醛质量摩尔浓度、超氧化物歧化酶活性、可溶性蛋白质量分数和叶绿素质量分数的变化。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

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