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The gels were then immediately scanned with a laser densitometer, and the readings of the samples corresponding to their total SOD activity were obtained by processing the image.

的凝胶,然后立刻扫了激光密度仪,而读的样本,相应的总超氧化物歧化酶活性得到处理图像。

The activities of superoxide dismutase and catalase, the contents of hydrogen peroxide (H2O2) and malonic dialdehyde as well as clinical symptoms and physical sign before and after treatment were determined.

通过观察SOD,Cat,H2O2,MDA及临床症状、体征的变化,对其疗效进行评价。

Result: The results showed that the serum lipids,superoxide dismutase and maleic dialdehyde of treated groups were obviously improved as compared with those of the controlgroup.The tissue pathologicaldamage was improved in different degree,and ICAM-1 and its expression of mRNA was decreased obviously.

结果:两治疗组血脂、血清MDA含量均明显降低,SOD活性显著升高,不同程度的改善了主动脉组织病理损伤,ICAM-1及其mRNA的表达水平显著下降。

Nevski were treated with different concentration of NaCl to investigate the changes of diastase activity, SOD activity, POD activity and soluble carbohydrate content during the germinating process.

不同浓度的NaCl处理芨芨草种子后,通过研究其萌发过程中淀粉酶、超氧化物歧化酶、过氧化物酶活性以及可溶性糖含量的变化。

The results showed that the wheat growth was increased with lower concentration of Cu; but with higher concentration of Cu, the activities of diastase and the mitotic index of root tip cell of wheat were reduced, the content of pro was increased.

朱云集等研究了Cu对小麦根系生长发育及生理特性的影响,指出随Cu浓度的提高,小麦胚根、次生根条数减少,根系SOD与POD活性呈下降趋势[7]。

We differentiated the sexual distinction of dioecism Vitis by observing the shape of pollen under a scanning electron microscope; analyzing the isozyme of SOD in leaves; detecting the polymorphism of genomie DNA with the method of RAPD.

通过扫描电镜观察花粉的形态;分析叶片超氧化物歧化酶同工酶;RAPD分析基因组DNA多态性,来鉴别葡萄的性别。

The probe connects rhodamine with artifical enzyme complex, and utilizes the conformational change of SOD active center during the dismutation of superoxide anion to realize the change of the fluorescent intensity of fluorophore.

过程中出现的活性部位结构变化实现荧光团的荧光强度变化,以期实现对O2-。

The rate constants and IC50 values of the dismutation of O2 catalyzed by these complexes have been determined by riboflavin-nitroblue tetrazolium illumination method.

采用核黄素-NBT光照法测定了配合物催化O_2~-歧化的反应速率常数K_Q以及IC_(50)值。结果表明,七种配合物具有相当好的SOD活性,它们的K_Q和IC_(50)值分别在4.08×10~6-1.65×10~7'mol·L~(-1)s~(-1)和1.42×10~(-6)-2.93×10~(-7)mol·L~(-1)范围之内。

Superoxide Dismutases are ubiquitously expressed antioxidant enzyme in aerobic organisms and catalyze dismutation of superoxide anion to hydrogen and molecular oxygen,the first step in active oxygen-scavenging systems.

超氧化物歧化酶(superoxide dismutase,SOD)在需氧原核生物和真核生物中广泛存在,是活性氧清除系统中第一个发挥作用的抗氧化酶。

Since the dismutation of superoxide radicals by SOD is a reaction that is rate-limited by diffusion and enhanced by electrostatic guidance, all these structural features may be related to GP-MnSOD's high catalytic activity: first, Lys38 may increase the effective enzyme-substrate association rate by enhancing the electrostatic gradient at its substrate channel; second, an excess of acidic over basic residues makes the negative surface potential of GP-MnSOD more larger than those of other known MnSODs. This may enhance the reaction rate by reducing futile collisions of superoxide radicals with GP-MnSOD's surface.

因此,GP-MnSOD的上述结构特征可能与GP-MnSOD的较高酶活有关:首先,Lys38增加了底物通道口的正静电势梯度,从而可能促进了GP-MnSOD的活性中心与底物的有效结合速率;其次,GP-MnSOD表面覆盖的过量酸性残基使得它比其它MnSOD在底物通道以外的蛋白表面更多地被负静电势所覆盖,这可能有效地减少了超氧阴离子与GP-MnSOD表面的无效碰撞,从而提高了反应速率。

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