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Low dose Yiweitang group; 4. Diethylstilbestrol group; 5. Model control group. After 4 weeks intervention, get the left ovarie, a part of hypothalami and pituitary gland from head-off rat, and observe expression of ER, FSHR and GnRH by immune histochemistry method . Then extract RNA from the rihgt ovarie and the rest hypothalami and pituitary gland, so as to measure the expression of GnRHmRNA 、 ERmRNA 、 FSHRmRNA and P 16mRNA by RT-PCR. And the cerebrum were made to homogenate to examine SOD, GSH-PX and MDA.

各组实验动物灌药4周后,断头处死,取部分下丘脑、垂体及左侧卵巢组织固定切片,免疫组化法检测 ER 和 FSHR 表达、 GnRH ;取剩余部分下丘脑、垂体及右侧卵巢组织提取总 RNA ,用于 RT-PCR 检测下丘脑 GnRHmRNA ,下丘脑、垂体的 P 16mRNA 及下丘脑、垂体、卵巢的 ERmRNA 、 FSHRmRNA 基因表达;另外取部分大脑组织制作脑组织匀浆,用于 SOD 和 GSH-PX 酶活力以及 MDA 含量的测定。

It is Investigated that the effects of vitamin C and E on M. nipponense in different molting stages by measuring oxygen consumption rates, ammonia-N excretion rates and activities of antioxidant enzymes(SOD,CAT,GSH-Px,GST,GR) in three groups which are control group,Vc group andgroup.1. The oxygen consumption rates are high in stages D2 and A, but low in stage C.

设立三个试验组:对照组,V_C组(200mgV_C/kg)和V_C+V_E组(200mgV_C/kg+400mg V_E/kg),以上述分期结果为标准,测定了三个试验组的日本沼虾在蜕皮各时期的耗氧率,排氨率以及抗氧化酶(超氧化物歧化酶SOD,过氧化氢酶CAT,谷胱甘肽过氧化物酶GSH-P_X,谷胱甘肽转移酶GST,谷胱甘肽还原酶GR)活性的变化,结果如下: 1。

With one-year-old dormant branches of four sweet cherry varieties as test materials, the activities of SOD and POD in different periods were measured.

方法]以4个甜樱桃品种1年生枝条为试材,测定不同时期的SOD、POD酶活性变化。

ObjectiveTo study the effect of natural ox gallstone on energy metabolism,activity of SOD and content of MDA in SHR brain.

目的研究天然牛黄对于自发高血压大鼠脑细胞外液能量代谢及海马组织SOD活性、MDA含量的影响。

ATP content was detected by high performance liquid chromatogram. Mitochondrial membrane potential was measured by rhodamine123 (Rh123) staining and photofluorography. The activity of superoxide dismutase was observed using a SOD kit. The expression of heme oxygenase-1 (HO-1) was evaluated by Western blotting.

应用高效液相色谱法检测细胞内ATP的含量;罗丹明123(Rh123)染色荧光显微镜照相检测线粒体膜电位;超氧化物歧化酶检测试剂盒检测SOD活性;免疫印迹法检测血红素氧合酶-1(HO-1)的表达。

Experimental Center of National Level One, Hubei Traditional Chinese Medica l College, Wuhan 430061)Abstract To investigate the protective effects of Jiaweisinisa n on acute liver injury induced by intraperitoneal injection of D-ga lac-tosamise, serum superoxide dismutase, malondialdehyde , glutathioe contents were measured. The pathological changes of liver wer e observed simultaneously. The results showed that JWSNS could decrease serum MD A content, but increase serum SOD and GSH activities in acute liver injury induc ed by D-GalN. It was also found that JWSNS could alleviate the degeneration and necrosis of the hypatocytes.

为研讨加味四逆散防治肝损伤的作用机理,通过动物试验,先用加味四逆散预防给药,再采用D-氨基半乳糖造成大鼠急性肝损伤模型,然后检测大鼠血清超氧化物岐化酶、丙二醛、还原型谷胱甘肽水平,同步观察该方对急性肝损伤大鼠体内脂质过氧化反应及肝脏病理改变的影响,结果表明该方可明显升高大鼠血清SOD、GSH水平,降低MDA水平,肝脏病理学检查亦表明该方使肝细胞变性坏死程度减轻。

After 8 weeks of administration,compared with the model group,the level of β-AP was decreased while the S.P was increased,the activity of SOD was significantly increased( P .01)and the histomorphology in the brain was improved in the HYP groups.

与模型组比较,给药组大鼠脑组织的β-AP含量下降和P物质水平增加,血清SOD水平显著升高(P.01),脑组织形态结构得到改善。

The model rats were cured for 30 days with tonifying liver QI formula, then the effects on blood lipids , the quantity of MDA, GSH, SOD and Hyp in liver was studied.

观察补益肝气方药对模型大鼠治疗30天后,对血清血脂含量,以及对肝组织中MDA、GSH、SOD、Hyp含量的影响。

To realize the inhibitory effect and the mechanism of LBP on calcium oxalate crystallization. Results: The score of renal calcium oxalate crystallization of LBP group (50 mg/kg·d, 100mg/kg·d) decreased 27.6% and 34.5%(P.05). SOD of renal increased 15.0% and 28.7%. MDA was reduced 28.9% and 35.6%. apoptosis index of kidney tubule epithelium was reduced 40.9% and 53.3%(P.05) compared with the ethylene glycol drinking group.

结果:与诱石组相比,枸杞多糖(50 mg/kg·d、100mg/kg·d)干预组的草酸钙结晶评分分别降低27.6%和34.5%(P.05),SOD分别增加15.0%和28.7%,MDA分别减少28.9%和35.6%,细胞凋亡指数分别减少40.9%和53.3%(P.05),12%、25%枸杞浸泡剂和煎剂的草酸钙结晶评分分别降低38.7%,54.8%和48.4%,58.1%(P.01)。

RESULTS Compared with normal control group, HVA, 5-HT and GSH contents, and SOD activity in corpus striatum of model mice decreased, and MDA content increased significantly. Otherwise, numbers of Bcl-2 and caspase 3 immune reaction positive cells in substantia nigra increased significantly.

结果 与正常对照组比较,模型组小鼠脑内纹状体HVA,5-HT和GSH含量显著下降,SOD活性下降,MDA含量明显升高;黑质致密部Bcl-2和caspase 3免疫阳性细胞数增加。

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