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During log-growth phase,the activity of SOD increases with culture time,and at the same time the activities of the other two enzymes also increase.

通过比较几种提取方法的效果,认为利用甲苯破壁法提取SOD复合酶具有一定的可行性。

The expressions of p53 and c-fos in the present experiment varied similarly to the apoptosis, i. e. decreased only when SOD was added to the culture simultaneously with the antitumor drugs.

DDP和ADM作用同时加入SOD共同孵育可降低Hela细胞凋亡率(P0.05),而且与p53和c-fos基因表达变化具有一致性。

Meanwhile or 24 h later, 316 mg.l-1 sod was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of hela cells were determined. results the production of ros and the apoptosis of hella cells increased as the action of ddp and adm continued within 24 h, but vp-16 failed to produce such effects.

宫颈癌hela细胞经顺铂、足叶乙甙(vp-16)和阿霉素血浆峰浓度不同时间作用后,测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化,并测定不同时间加入sod对ddp、adm、vp-16诱导hela细胞凋亡及p53、c-fos基因表达的影响。

L^(-1) VP-16 for 6, 12, 24 and 48 h. Meanwhile or 24 h later, 316 mg?L^(-1) SOD was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of Hela cells were determined. Results The production of ROS and the apoptosis of Hella cells increased as the action of DDP and ADM continued within 24 h, hut VP-16 failed to produce such effects.

宫颈癌Hela细胞经顺铂、足叶乙甙(VP-16)和阿霉素血浆峰浓度不同时间作用后,测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化,并测定不同时间加入SOD对DDP、ADM、VP-16诱导Hela细胞凋亡及p53、c-fos基因表达的影响。

Results: Compared with I/R+insulin group and I/R+pioglitazone group. the ultrastructure of cardiomyocytes suffering ischemia reperfusion injury recovered markedly, the fluorescence intensity of the intracellular calcium ion decreased, the survival rate of cardiomyocytes increased, LDH, CK, MDA concentration in culture medium decreased and SOD concentration in culture medium increased in I/R+insulin+pioglitazone group.

结果:吡格列酮与胰岛素联合应用可提高缺血再灌注损伤心肌细胞的存活率,降低细胞培养液中的LDH、CK和MDA含量,提高SOD的含量,降低心肌细胞钙离子染色的荧光强度,部分恢复心肌细胞的超微结构,与单独应用吡格列酮或胰岛素相比,具有统计学意义。

LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochchondral respiration were evaluated in each group; apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis; the immuneohistochemical method was used to determine the expression of bcl-2 and Caspase-3 ; the morphoiogic changes were observed with microscope.

将培养的L-6TG大鼠肌母细胞随机分为3组:①正常对照组,②缺血再灌注组(1R组),③缺血预处理组,观测了培养上清中LDH、细胞内SOD、XOD、Ca(上标 2+)含量的变化;采用MTT法检测线粒体的功能;利用流式细胞仪和细胞DNA电泳结果检测细胞凋亡情况;采用免疫组织化学的方法检测bcl-2及Caspase-3的蛋白表达情况,结合自动图像分析系统对其结果进行定量分析;在光镜下观察细胞的形态学改变。

The seed vigor, SOD and POD decreased significantly;and electric conductivity, the content of soluble sugar and MDA increased significantly.

而超干处理的种子贮藏18个月后,种子的发芽率、发芽指数、活力指数、电导率、可溶性糖含量、SOD活性、POD活性和MDA含量均无显著变化。

The results showed that with the immersion time of straw and straw leaching solution concentration increased, the germination rate of wheat seed decreases, the time of emergence of wheat seed also will extend the POD activity of wheat seedlings, SOD activity and root activity show a downward trend , leaf MDA content was increased.

结果表明,随着秸秆浸泡时间的延长和秸秆浸出液浓度的增加,小麦种子的萌发率随之降低,小麦种子的出苗时间也随之延长,小麦幼苗POD活性增加,SOD活性和根系活力呈现下降趋势,叶片MDA含量却升高。

The total SOD activity of an unknown sample could be obtained by interpolating its reading to the standard curve.

总SOD的一个未知样品可插读它的标准曲线。

Effects of different concentrations of exogenous SOD on antioxidant enzyme activities and sugar contents in leaf and fruit were studied, taking tetraploid plant in Vitis vinifera cv 'Muscat humburg' as materials and water treatment as the CK.

以四倍体玫瑰香葡萄为试材,以喷清水为对照,探讨了不同浓度外源SOD对叶片、果实抗氧化酶活性和含糖量的影响。

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