查询词典 sod culture

With sod culture in orchards as background and the native arbuscular mycorrhizal fungi in orchard soil as target, the influences of Stylosanthes gracilis, Ageratum conyzoides and Paspalum notatum on the growth and community structure of AM fungi were investigated.

以果园生草栽培为背景，果园土壤中土著AM真菌为研究对象，考察了柱花草、百喜草和藿香蓟对与之根系共生的AM真菌的生长与群落结构的影响。

The results of MTT method In vitro culture renal tubular cells showed that the proliferous activity changed from sightly to dramatically intensed in response to fluoride concentration from 0.1mg/L to 15mg/L, but renal tubular cells proliferation obviously decreased in the 25mg F-/L group.

利用生物化学方法检测氧化应激指标，结果表明在钙营养充分的条件下，投氟100mg/L 16周大鼠肾组织GPX活性不是下降而是明显提高，脂质过氧化物变化不明显；低钙饲养条件下投氟100mg/L 16周大鼠抗氧化酶SOD活性亦有明显升高，而GPX却呈降低趋势。

Hyphal growth rate method was used to determine the inhibitory activities of chitooligosancharide against the pathogenic fungus of tobacco brown spot, Alternaria alternate Keissler, in the laboratory, and tobacco plants were applied with COS and its combinations like COS + Trichoderma + chitin with 5 treatments to control the disease under conditions of pot culture and inoculation in the greenhouse. Activities of 4 defensive enzymes, superoxide dismutase, polyphenol oxidase, peroxidase and chitinase, in tobacco plants were determined systematically at the same time within 10 days after application of COS and inoculation with the pathogenic fungus.

在实验室条件下，采用菌丝生长速率法测定了几丁寡糖对烟草赤星病菌的抑制活性，继而在温室盆栽和人工接种条件下，分别研究了几丁寡糖、几丁寡糖+木霉+几丁质等5种方法处理对烟草赤星病的防治效果，同时分别系统测定了烟草植株施用几丁寡糖及人工接种赤星病菌后10 d内SOD、POD、PPO和几丁质酶等4种防御酶的活性。

Our results indicate that the community structure of native AM fungi in orchard soil differed from each other when colonized with different grass species, which should be taken into account when selecting appropriate grass species for sod culture.

试验表明，在果园土壤中，不同草种根际的土著AM真菌群落结构并不相同，在选择草种进行生草栽培的过程中应该考虑这些不同。

During log-growth phase,the activity of SOD increases with culture time,and at the same time the activities of the other two enzymes also increase.

通过比较几种提取方法的效果，认为利用甲苯破壁法提取SOD复合酶具有一定的可行性。

The expressions of p53 and c-fos in the present experiment varied similarly to the apoptosis, i. e. decreased only when SOD was added to the culture simultaneously with the antitumor drugs.

DDP和ADM作用同时加入SOD共同孵育可降低Hela细胞凋亡率(P0.05)，而且与p53和c-fos基因表达变化具有一致性。

Meanwhile or 24 h later, 316 mg.l-1 sod was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of hela cells were determined. results the production of ros and the apoptosis of hella cells increased as the action of ddp and adm continued within 24 h, but vp-16 failed to produce such effects.

宫颈癌hela细胞经顺铂、足叶乙甙（vp-16）和阿霉素血浆峰浓度不同时间作用后，测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化，并测定不同时间加入sod对ddp、adm、vp-16诱导hela细胞凋亡及p53、c-fos基因表达的影响。

L^(-1) VP-16 for 6, 12, 24 and 48 h. Meanwhile or 24 h later, 316 mg?L^(-1) SOD was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of Hela cells were determined. Results The production of ROS and the apoptosis of Hella cells increased as the action of DDP and ADM continued within 24 h, hut VP-16 failed to produce such effects.

宫颈癌Hela细胞经顺铂、足叶乙甙(VP-16)和阿霉素血浆峰浓度不同时间作用后，测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化，并测定不同时间加入SOD对DDP、ADM、VP-16诱导Hela细胞凋亡及p53、c-fos基因表达的影响。

LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochchondral respiration were evaluated in each group; apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis; the immuneohistochemical method was used to determine the expression of bcl-2 and Caspase-3 ; the morphoiogic changes were observed with microscope.

将培养的L-6TG大鼠肌母细胞随机分为3组：①正常对照组，②缺血再灌注组（1R组），③缺血预处理组，观测了培养上清中LDH、细胞内SOD、XOD、Ca(上标 2+)含量的变化；采用MTT法检测线粒体的功能；利用流式细胞仪和细胞DNA电泳结果检测细胞凋亡情况；采用免疫组织化学的方法检测bcl-2及Caspase-3的蛋白表达情况，结合自动图像分析系统对其结果进行定量分析；在光镜下观察细胞的形态学改变。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。